Laboratory extraction of microalgal lipids using sugarcane bagasse derived green solvents

Jesus, Sérgio S. de; Ferreira, Gabriela F.; Fregolente, Leonardo Vasconcelos; Filho, Rubens Maciel

doi:10.1016/j.algal.2018.09.001

Cited by 26 publications

(11 citation statements)

References 29 publications

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“…MAG percentage increased significantly when PLE temperature was 150 °C (differences observed at 5% level) ( Figure 5 ), and so, the optimal temperature was selected as 120 °C, despite a lower total lipid recovery. On the other hand, a decreasing affinity for polar lipids with increasing temperature could be observed for the 2-MeTHF extracts, as has also been described in other works [ 18 , 22 , 23 ].…”

Section: Discussionsupporting

confidence: 85%

“…In this work, a new sugarcane or corn biomass derived solvent, 2-methyl-tetrahydrofuran (or 2-MeTHF, CAS No. 96-47-9) was studied as a promising substitute of non-polar conventional solvents such as hexane [ 17 , 18 ], studying mixtures with ethanol, isopropanol, and isobutanol for the advanced extraction of microalgal lipids. As responses to optimize extraction, obtained oil yield, relative percentage of neutral and polar lipids (GL) analyzed by HPLC-ELSD, and omega-3 fatty acids (DHA and EPA) composition in the samples analyzed by GC-MS, were considered.…”

Section: Introductionmentioning

confidence: 99%

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Biobased Solvents for Pressurized Liquid Extraction of Nannochloropsis gaditana Omega-3 Lipids

Blanco-Llamero

Señoráns

2021

Marine Drugs

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To develop greener extraction alternatives for microalgae biomass, ultrasound assisted extraction (UAE) and pressurized liquid extraction (PLE) with different biobased solvents were investigated, demonstrating that both techniques are useful alternatives for algal lipid extraction. Specifically, Nannochloropsis gaditana lipids were extracted by UAE and PLE at different temperatures and extraction times with sustainable solvents like 2-Methyltetrahydrofuran (2-MeTHF) and its mixtures with ethanol and other alcohols. The best oil yields for both PLE and UAE of N. gaditana were achieved with the mixture of 2-MeTHF:ethanol (1:3), reaching yields of up to 16.3%, for UAE at 50 °C and up to 46.1% for PLE at 120 °C. Lipid composition of the extracts was analyzed by HPLC-ELSD and by GC-MS to determine lipid species and fatty acid profile, respectively. Different fractionation of lipid species was achieved with PLE and solvent mixtures of different polarity. Thus, for the extraction of glycolipids, ethanolic extracts contained higher amounts of glycolipids and EPA, probably due to the higher polarity of the solvent. The optimized method was applied to microalgae Isochrysis galbana and Tetraselmis chuii showing the potential of mixtures of biobased solvents like 2-methyl-THF and ethanol in different proportions to efficiently extract and fractionate lipids from microalgal biomass.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Biobased Solvents for Pressurized Liquid Extraction of Nannochloropsis gaditana Omega-3 Lipids

Blanco-Llamero

Señoráns

2021

Marine Drugs

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“…Another example is the application of methyltetrahydrofuran for the extraction of steroids from rapeseeds as efficiently as n -hexane [33]. A chloroform–methanol mixture or n -hexane can be replaced with 2-methyltetrahydrofuran–isoamyl alcohol or 2-methyltetrahydrofuran–ethanol [34], or with ethanol and ethyl acetate [35], for the extraction of lipids from microalgae.…”

Section: Biosolvents Used In Analytical Chemistrymentioning

confidence: 99%

Analytical chemistry with biosolvents

Tobiszewski

2019

Anal Bioanal Chem

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One of the current trends in green analytical chemistry is the introduction of green solvents, some of which are biobased. At the same time, the development of the biorefinery concept has allowed more biochemicals to be obtained with increased efficiency and from a wider range of feedstocks. The first examples of the use of biosolvents in analytical applications included extractions performed with alcohols, esters, and terpenes. However, many more applications of biosolvents in extractions of bioactive compounds from various plant materials have also been reported, which hints at a wider range of potential analytical applications of biosolvents. It should also be noted that the biobased solvents applied in analytical chemistry are not always green, as some of them are toxic towards aquatic organisms. Graphical abstract

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“…In recent decades, a new class of solvents has emerged as an alternative and is called “green solvents” [ 7 , 8 ] that are less harmful to human health and the environment without compromising lipid quality. Recently, laboratory tests have shown that different green solvents have the potential to substitute chloroform and hexane in the recovery of lipids, mainly in the extraction of oilseeds and oil with high biological value, such as tocopherols and sterols [ 9 , 10 , 11 ]. Indeed, 2 -methyltetrahydrofuran (MeTHF) is one of the green solvents with a high potential to replace n-hexane.…”

Section: Introductionmentioning

confidence: 99%

Green Solvent to Substitute Hexane for Bioactive Lipids Extraction from Black Cumin and Basil Seeds

Bourgou

Rebey

Kaab

et al. 2021

Foods

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A comparative study of bioactive lipids extraction from black cumin (Nigella sativa L.) and basil (Ocimum basilicum L.) seeds using conventional petroleum-based solvent and green solvent 2-methyltetrahydrofuran (MeTHF) was performed. MeTHF extraction allowed obtaining the highest oil yield in black cumin (34%). Regarding fatty acids composition, linoleic acid (61%) and α-linolenic (78%) were relevant in black cumin and basil green and conventionally extracted oils, respectively. Besides, MeTHF allowed obtaining higher tocopherols and total phenolics contents in black cumin (400 mg/kg of oil and 12 mg EGA/g oil) and basil (317 mg/kg oil and 5 mg EGA/g oil) compared to hexane-extracted ones. The content of major phenolic compounds in the two seed oils, trans-hydroxycinnamic acid, rosmarinic acid, and thymol was enhanced by MeTHF extraction. Furthermore, MeTHF-extracted oils possess stronger antioxidant activities (radical scavenging, total antioxidant, and β-carotene bleaching activities) and high and similar anti-inflammatory capacity to hexane-extracted oils. In conclusion, the results revealed that MeTHF is efficient to replace hazardous solvents to extract oil from black cumin and basil seeds rich in compounds relevant to the human diet, including essential polyunsaturated fatty acids (n-6 and n-3), tocopherols, and phenolic compounds with improved biological activities.

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Laboratory extraction of microalgal lipids using sugarcane bagasse derived green solvents

Cited by 26 publications

References 29 publications

Biobased Solvents for Pressurized Liquid Extraction of Nannochloropsis gaditana Omega-3 Lipids

Biobased Solvents for Pressurized Liquid Extraction of Nannochloropsis gaditana Omega-3 Lipids

Analytical chemistry with biosolvents

Green Solvent to Substitute Hexane for Bioactive Lipids Extraction from Black Cumin and Basil Seeds

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