Laboratory formulated magnetic nanoparticles for enhancement of viral gene expression in suspension cell line

Bhattarai, Shanta Raj; Kim, Sun Young; Jang, Kyu Yun; Lee, Ki Chang; Yi, Ho Keun; Lee, Dae Yeol; Kim, Hee Young; Hwang, Pyoung Han

doi:10.1016/j.jviromet.2007.08.028

Cited by 50 publications

(20 citation statements)

References 10 publications

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“…Numerous publications describe nanoparticles with positive surface charge which are internalized into cells more efficiently than those with negative surfaces. [31][32][33] Some studies, however, show an uptake behavior that is just the opposite. 34,35 Despite the fact that the cellular membrane is in general negatively charged, it does have some areas with cationic sites which allow the binding of anionic nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist – an initial in vitro study

Skopalík¹,

Poláková²,

Havrdová³

et al. 2014

IJN

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Objective Cell therapies have emerged as a promising approach in medicine. The basis of each therapy is the injection of 1–100×10 6 cells with regenerative potential into some part of the body. Mesenchymal stromal cells (MSCs) are the most used cell type in the cell therapy nowadays, but no gold standard for the labeling of the MSCs for magnetic resonance imaging (MRI) is available yet. This work evaluates our newly synthesized uncoated superparamagnetic maghemite nanoparticles (surface-active maghemite nanoparticles – SAMNs) as an MRI contrast intracellular probe usable in a clinical 1.5 T MRI system. Methods MSCs from rat and human donors were isolated, and then incubated at different concentrations (10–200 μg/mL) of SAMN maghemite nanoparticles for 48 hours. Viability, proliferation, and nanoparticle uptake efficiency were tested (using fluorescence microscopy, xCELLigence analysis, atomic absorption spectroscopy, and advanced microscopy techniques). Migration capacity, cluster of differentiation markers, effect of nanoparticles on long-term viability, contrast properties in MRI, and cocultivation of labeled cells with myocytes were also studied. Results SAMNs do not affect MSC viability if the concentration does not exceed 100 μg ferumoxide/mL, and this concentration does not alter their cell phenotype and long-term proliferation profile. After 48 hours of incubation, MSCs labeled with SAMNs show more than double the amount of iron per cell compared to Resovist-labeled cells, which correlates well with the better contrast properties of the SAMN cell sample in T2-weighted MRI. SAMN-labeled MSCs display strong adherence and excellent elasticity in a beating myocyte culture for a minimum of 7 days. Conclusion Detailed in vitro tests and phantom tests on ex vivo tissue show that the new SAMNs are efficient MRI contrast agent probes with exclusive intracellular uptake and high biological safety.

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Section: Discussionmentioning

confidence: 99%

Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist – an initial in vitro study

Skopalík¹,

Poláková²,

Havrdová³

et al. 2014

IJN

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“…Despite the success of viral vectors as transfection (transduction) agents [6], over the past decade there has been an increasing shift towards the use of nonviral gene trans fection. This is due to concerns associated with the use of viruses, mostly regarding safety such as induced inflammatory response and insertional mutagenesis, but also owing to limitations in the plasmid packaging capacity of the different viral vectors [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Oscillating Magnet Array-Based Nanomagnetic Gene Transfection of Human Mesenchymal Stem Cells

Fouriki

Dobson

2014

Nanomedicine

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“…Despite the success of viral vectors at transfecting a variety of primary cells and cell lines, over the past decade, there has been an increasing shift towards the use of non-viral gene transfection due to viral-associated concerns, mostly regarding safety, such as induced inflammatory response and mutagenesis, as well as limitations on the plasmid size [4,5,6,7,8]. …”

Section: Introductionmentioning

confidence: 99%

Nanomagnetic Gene Transfection for Non-Viral Gene Delivery in NIH 3T3 Mouse Embryonic Fibroblasts

Fouriki

Dobson

2013

Materials

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The objective of this work was to examine the potential of oscillating nanomagnetic gene transfection systems (magnefect-nano™) for improving the transfection efficiency of NIH3T3 mouse embryonic fibroblasts (MEFs) in comparison to other non-viral transfection techniques—static magnetofection™ and the cationic lipid agent, Lipofectamine 2000™. Magnetic nanoparticles (MNPs) associated with the plasmid coding for green fluorescent protein (GFP) were used to transfect NIH3T3 cells. The magnefect-nano system was evaluated for transfection efficiency, and any potential associated effects on cell viability were investigated. MNPs associated with the plasmid coding for GFP were efficiently delivered into NIH3T3 cells, and the magnefect-nano system significantly enhanced overall transfection efficiency in comparison to lipid-mediated gene delivery. MNP dosage used in this work was not found to affect the cell viability and/or morphology of the cells. Non-viral transfection using MNPs and the magnefect-nano system can be used to transfect NIH3T3 cells and direct reporter gene delivery, highlighting the wide potential of nanomagnetic gene transfection in gene therapy.

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Laboratory formulated magnetic nanoparticles for enhancement of viral gene expression in suspension cell line

Cited by 50 publications

References 10 publications

Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist – an initial in vitro study

Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist – an initial in vitro study

Oscillating Magnet Array-Based Nanomagnetic Gene Transfection of Human Mesenchymal Stem Cells

Nanomagnetic Gene Transfection for Non-Viral Gene Delivery in NIH 3T3 Mouse Embryonic Fibroblasts

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Laboratory formulated magnetic nanoparticles for enhancement of viral gene expression in suspension cell line

Cited by 50 publications

References 10 publications

Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist &ndash; an initial in vitro study

Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist &ndash; an initial in vitro study

Oscillating Magnet Array-Based Nanomagnetic Gene Transfection of Human Mesenchymal Stem Cells

Nanomagnetic Gene Transfection for Non-Viral Gene Delivery in NIH 3T3 Mouse Embryonic Fibroblasts

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Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist – an initial in vitro study

Mesenchymal stromal cell labeling by new uncoated superparamagnetic maghemite nanoparticles in comparison with commercial Resovist – an initial in vitro study