Oscillating Magnet Array-Based Nanomagnetic Gene Transfection of Human Mesenchymal Stem Cells

Fouriki, Angeliki; Dobson, Jon

doi:10.2217/nnm.13.74

Cited by 19 publications

(14 citation statements)

References 34 publications

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“…Like gold, other inorganic nanoparticles can also facilitate simultaneous imaging and therapeutic delivery. Magnetic nanoparticles are easily and scalably manufactured, and they can be detected by MRI for tracking or diagnostic purposes as well as moved toward target cells using a magnetic field . Quantum dots (QDs) are an interesting option as a delivery vehicle with optical properties, as they are designed to be bright, photostable imaging agents and are normally of small size amenable to in vivo trafficking and cellular uptake .…”

Section: Nanomaterials For Combinatorial Nucleic Acid Deliverymentioning

confidence: 99%

“…Magnetic nanoparticles are easily and scalably manufactured, and they can be detected by MRI [357] for tracking or diagnostic purposes as well as moved toward target cells using a magnetic field. [358] Quantum dots (QDs) are an interesting option as a delivery vehicle with optical properties, as they are designed to be bright, photostable imaging agents and are normally of small size amenable to in vivo trafficking and cellular uptake. [359,360] As with other inorganic nanoparticles mentioned here, many of these are generally used in combination with other gene delivery materials, such as coatings of PEI, [361] PEG, [362] and other polymers [363,364] to allow binding and other capabilities required for gene delivery.…”

Section: Inorganic Nanoparticlesmentioning

confidence: 99%

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Cancer‐Targeting Nanoparticles for Combinatorial Nucleic Acid Delivery

2019

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Nucleic acids are a promising type of therapeutic for the treatment of a wide range of conditions, including cancer, but they also pose many delivery challenges. For efficient and safe delivery to cancer cells, nucleic acids must generally be packaged into a vehicle, such as a nanoparticle, that will allow them to be taken up by the target cells and then released in the appropriate cellular compartment to function. As with other types of therapeutics, delivery vehicles for nucleic acids must also be designed to avoid unwanted side effects; thus, the ability of such carriers to target their cargo to cancer cells is crucial. Classes of nucleic acids, hurdles that must be overcome for effective intracellular delivery, types of nonviral nanomaterials used as delivery vehicles, and the different strategies that can be employed to target nucleic acid delivery specifically to tumor cells are discussed. Additonally, nanoparticle designs that facilitate multiplexed delivery of combinations of nucleic acids are reviewed.

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Section: Nanomaterials For Combinatorial Nucleic Acid Deliverymentioning

confidence: 99%

Section: Inorganic Nanoparticlesmentioning

confidence: 99%

Cancer‐Targeting Nanoparticles for Combinatorial Nucleic Acid Delivery

2019

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“…The rationale for using both particles is that the study was aimed at evaluation of MNP + magnet array-mediated cancer cytotoxicity. Our goal was to demonstrate that the technology works efficiently with different commercially available magnetic nanoparticles that have been widely demonstrated to show high biocompatibility [10,17]. MNPs were diluted in 100 µl medium and added to cell cultures (0.2 µl per well) immediately prior to magnetic field application.…”

Section: Magnetic Field Gradient-mediated Cytotoxicity Experimentsmentioning

confidence: 99%

Remote manipulation of magnetic nanoparticles using magnetic field gradient to promote cancer cell death

et al. 2019

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The manipulation of magnetic nanoparticles (MNPs) using an external magnetic field, has been successfully demonstrated in various biomedical applications. Some have utilised this non-invasive external stimulus and there is an potential to build on this platform. The focus of this study is to understand the manipulation of MNPs by a time-varying static magnetic field and how, at different frequencies and displacement, this can alter cellular function. Here we explore, using numerical modeling, the physical mechanism which underlies this process, and we discuss potential improvements for its use in biomedical applications. From our data and other related studies, we infer that such phenomenon largely depends on the magnetic field gradient, magnetic susceptibility and size of the MNPs, magnet array oscillating frequency, viscosity of the medium surrounding MNPs, and distance between the magnetic field source and MNPs. Additionally, we demonstrate cytotoxicity in neuroblastoma (SH-SY5Y) and hepatocellular carcinoma (HepG2) cells in vitro induced by MNPs exposed to a magnetic field gradient and oscillating at various frequencies and displacement amplitudes. Even though this technique reliably produces MNP endocytosis and/or cytotoxicity, a better understanding is required to develop this system for precision manipulation of MNPs, ex vivo.

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“…The rationale for using both particles is that the study was aimed at evaluation of MNP + magnet array mediated cancer cytotoxicity. Our goal was to demonstrate that the technology works efficiently with different commercially available magnetic nanoparticles that have been widely demonstrated to show high biocompatibility [Subramanian, M., et al, 2017;Fouriki, A. and Dobson, J., 2014]. MNPs were prediluted in 100 μ l medium and added to cell cultures (0.2 μ l per well) immediately prior to magnetic field application.…”

Section: Magnetic Field Gradient Mediated Cytotoxicity Experimentsmentioning

confidence: 99%

Remote manipulation of magnetic nanoparticles using magnetic field gradient to promote cancer cell death

Subramanian

Miaskowski

Jenkins

et al. 2018

Preprint

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The manipulation of magnetic nanoparticles (MNPs) using an external magnetic field, has been demonstrated to be useful in various biomedical applications. Some techniques have evolved utilizing this non-invasive external stimulus but the scientific community widely adopts few, and there is an excellent potential for more novel methods. The primary focus of this study is on understanding the manipulation of MNPs by a time-varying static magnetic field and how this can be used, at different frequencies and displacement, to manipulate cellular function.Here we explore, using numerical modeling, the physical mechanism which underlies this kind of manipulation, and we discuss potential improvements which would enhance such manipulation with its use in biomedical applications, i.e., increasing the MNP response by improving the field parameters. From our observations and other related studies, we infer that such manipulation depends mostly on the magnetic field gradient, the magnetic susceptibility and size of the MNPs, the magnet array oscillating frequency, the viscosity of the medium surrounding MNPs, and the distance between the magnetic field source and the MNPs. Additionally, we demonstrate cytotoxicity in neuroblastoma (SH-SY5Y) and hepatocellular carcinoma (HepG2) cells in vitro. This was induced by incubation with MNPs, followed by exposure to a magnetic field gradient, physically oscillating at various frequencies and displacement amplitudes. Even though this technique reliably produces MNP endocytosis and/or cytotoxicity, a better biophysical understanding is required to develop the mechanism used for this precision manipulation of MNPs, in vitro.

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Oscillating Magnet Array-Based Nanomagnetic Gene Transfection of Human Mesenchymal Stem Cells

Abstract: Nonviral transfection using MNPs and oscillating magnet arrays offers a more efficient and 'cell-friendly' method of transfecting hMSCs than other nonviral techniques, while preserving their stem cell characteristics.

Cited by 19 publications

References 34 publications

Cancer‐Targeting Nanoparticles for Combinatorial Nucleic Acid Delivery

Cancer‐Targeting Nanoparticles for Combinatorial Nucleic Acid Delivery

Remote manipulation of magnetic nanoparticles using magnetic field gradient to promote cancer cell death

Remote manipulation of magnetic nanoparticles using magnetic field gradient to promote cancer cell death

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