Laboratory study of several enrichment broths for the detection of Salmonella spp. particularly in relation to water samples

Moriñigo, Miguel A.; Muñoz, Manuel; Martínez-Manzanares, Eduardo; Sánchez, José María Escobar; Borrego, Juan J.

doi:10.1111/j.1365-2672.1993.tb03033.x

Cited by 21 publications

(8 citation statements)

References 22 publications

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“…Paratyphi B isolate. The culture media we used is reported to inhibit growth of Salmonella serovars that cause typhoid or paratyphoid fever [ 50 ]. A study in Uganda reported S .…”

Section: Discussionmentioning

confidence: 99%

Potential Sources and Transmission of Salmonella and Antimicrobial Resistance in Kampala, Uganda

et al. 2016

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In sub‒Saharan Africa, non‒typhoidal Salmonellae (NTS) cause invasive disease particularly in children and HIV infected adults, but the disease epidemiology is poorly understood. Between 2012 and 2013, we investigated NTS sources and transmission in Kampala. We detected Salmonella in 60% of the influent and 60% of the effluent samples from a wastewater treatment plant and 53.3% of the influent and 10% of the effluent samples from waste stabilization ponds that serve the human population; 40.9% of flush‒water samples from ruminant slaughterhouses, 6.6% of the poultry fecal samples from live bird markets and 4% of the fecal samples from swine at slaughter; and in 54.2% of the water samples from a channel that drains storm–water and effluents from the city. We obtained 775 Salmonella isolates, identified 32 serovars, and determined resistance to 15 antimicrobials. We genotyped common serovars using multiple‒locus variable number tandem repeats analysis or pulsed‒field gel electrophoresis. In addition, we analyzed 49 archived NTS isolates from asymptomatic livestock and human clinical cases. Salmonella from ruminant and swine sources were mostly pan‒susceptible (95%) while poultry isolates were generally more resistant. Salmonella Kentucky isolated from poultry exhibited extensive drug resistance characterized by resistance to 10 antimicrobials. Interestingly, similar genotypes of S. Kentucky but with less antimicrobial resistance (AMR) were found in poultry, human and environmental sources. The observed AMR patterns could be attributed to host or management factors associated with production. Alternatively, S. Kentucky may be prone to acquiring AMR. The factors driving AMR remain poorly understood and should be elucidated. Overall, shared genotypes and AMR phenotypes were found in NTS from human, livestock and environmental sources, suggesting zoonotic and environmental transmissions most likely occur. Information from this study could be used to control NTS transmission.

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“…Paratyphi B isolate. The culture media we used is reported to inhibit growth of Salmonella serovars that cause typhoid or paratyphoid fever [ 50 ]. A study in Uganda reported S .…”

Section: Discussionmentioning

confidence: 99%

Potential Sources and Transmission of Salmonella and Antimicrobial Resistance in Kampala, Uganda

et al. 2016

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“…Generally, Salmonella sp. infections are detected by the isolation and identification of the bacteria from fecal cultures after several cultivation steps [4][5][6]. The identification is based on phenotypic and biochemical properties of the respective bacteria.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of Salmonella sp. by means of a combination of selective enrichment broth and MALDI-TOF MS

Sparbier

Weller

Boogen

et al. 2011

Eur J Clin Microbiol Infect Dis

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The identification of Salmonella sp. in stool samples usually takes 2 days when employing routine procedures. Fast approaches are necessary in order to shorten the analysis time. The aim of this work was the development of a rapid procedure for the detection of Salmonella sp. from clinical stool samples. Spiked stool samples were cultured in selective selenite enrichment broth. Identifications were directly performed from the liquid broth by the MALDI Biotyper. After the evaluation of this method, the same procedure was applied to clinical samples. Coevally, the samples were streaked on Hektoen agar and single colonies were analyzed by the MALDI Biotyper. For comparison, the liquid broth was plated according to the standard laboratory procedure. A total of 4,847 samples were analyzed for Salmonella sp. In total, 108 Salmonella sp.-positive samples were identified; 66 of these were identified after the streaking of stool samples on Hektoen agar and subsequent MALDI Biotyper analysis of Salmonella sp. suspicious colonies. These and a further 34 samples were detected as Salmonella sp.-positive directly from the selenite enrichment broth on day one. Eight Salmonella sp.-positive samples were not detected before plating of the selenite broth and subsequent MALDI Biotyper analysis on day two. The combination of MALDI Biotyper analysis and selective selenite enrichment broth identification delivers positive results for the majority of the samples already after one day.

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“…However, if enough blood is cultured it may be possible to increase the sensitivity of blood culture to that of bone marrow culture, thus avoiding the need for bone marrow aspiration. For stool culture, enrichment media containing selenite have been shown to be more effective than other available enrichment media [13,14]. Selenite with mannitol (selenite M) has been recommended [15] but there have been no large-scale comparisons of different selenite containing enrichment broths.…”

Section: Introductionmentioning

confidence: 99%

Specimens and culture media for the laboratory diagnosis of typhoid fever

Wain

Diep

Bay

et al. 2008

J Infect Dev Ctries

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Background: Culture of S. Typhi is necessary for the definitive diagnosis of typhoid fever and provides isolates for antibiotic susceptibility testing and epidemiological studies. However, current methods are not fully optimised and sourcing culture media and bottles for culture media may be problematic. Methodology: In two hospital laboratories in Viet Nam, comparisons of media for blood and stool culture were conducted. The effect of the volume of blood or stool on culture positivity rate was examined and direct plating of the blood buffy coat was trialed. Results: For 148 suspected typhoid fever cases, ox bile broth (58 positive) and brain-heart infusion broth containing saponin (63 positive), performed equally well. For 69 confirmed adult typhoid fever cases, large-volume (15ml) blood culture gave the same sensitivity as 1 ml of bone marrow culture. For 44 confirmed typhoid fever cases, the direct plating of the buffy coat was positive in 28 cases. For 263 positive stool cultures, selenite F and selenite mannitol performed equally well and culturing 2 g rather than 1g increased the isolation rate by 10.5%. Conclusions: For the diagnosis of typhoid fever by blood culture the medium should be a rich nutrient broth containing a lysing agent. In adults 1 ml bone marrow or 15 ml blood culture gave similar results. Where isolates are needed for susceptibility testing or epidemiological studies, but resources for culture are scarce, direct plating of the blood buffy coat can be used with a 50% fall in sensitivity compared to standard blood culture.

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Laboratory study of several enrichment broths for the detection of Salmonella spp. particularly in relation to water samples

Cited by 21 publications

References 22 publications

Potential Sources and Transmission of Salmonella and Antimicrobial Resistance in Kampala, Uganda

Potential Sources and Transmission of Salmonella and Antimicrobial Resistance in Kampala, Uganda

Rapid detection of Salmonella sp. by means of a combination of selective enrichment broth and MALDI-TOF MS

Specimens and culture media for the laboratory diagnosis of typhoid fever

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