Laboratory testing of larvivorous fish Japanese medaka (Oryzias latipes) predation ability to copper-treated Anopheles stephensi larvae: An alternative method for vector control under low concentration of copper

Reza, Mohamad; Yamamoto, Daisuke; Matsuoka, Hiroyuki

doi:10.7601/mez.64.67

Cited by 3 publications

(5 citation statements)

References 15 publications

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“…In fact, due to copper's effects on antipredator behavior, it has even been tested as a potential treatment for malaria eradication via vector control. Reza et al (2013) suggest the use of 0.26 mg/L Cu for larval Anopheles stephensi mosquito control because it significantly increases predation rates by medaka (Oryzias latipes). We did, however, find that larval period can be significantly lengthened by the elevated Cu levels in the absence of predator cues.…”

Section: Discussionmentioning

confidence: 99%

Effects of metal and predator stressors in larval southern toads (Anaxyrus terrestris)

2016

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Natural and anthropogenic stressors typically do not occur in isolation; therefore, understanding ecological risk of contaminant exposure should account for potential interactions of multiple stressors. Realistically, common contaminants can also occur chronically in the environment. Because parental exposure to stressors may cause transgenerational effects on offspring, affecting their ability to cope with the same or novel environmental stressors, the exposure histories of generations preceding that being tested should be considered. To examine multiple stressor and parental exposure effects we employed a 2 × 2 × 2 factorial design in outdoor 1000-L mesocosms (n = 24). Larval southern toads (Anaxyrus terrestris), bred from parents collected from reference and metal-contaminated sites, were exposed to two levels of both an anthropogenic (copper-0, 30 µg/L Cu) and natural (predator cue - present/absent) stressor and reared to metamorphosis. Toads from the metal-contaminated parental source population were smaller at metamorphosis and had delayed development; i.e., a prolonged larval period. Similarly, larval Cu exposure also reduced size at metamorphosis and prolonged the larval period. We, additionally, observed a significant interaction between larval Cu and predator-cue exposure on larval period, wherein delayed emergence was only present in the 30-µg/L Cu treatments in the absence of predator cues. The presence of parental effects as well as an interaction between aquatic stressors on commonly measured endpoints highlight the importance of conducting multistressor studies across generations to obtain data that are more relevant to field conditions in order to determine population-level effects of contaminant exposure.

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Section: Discussionmentioning

confidence: 99%

Effects of metal and predator stressors in larval southern toads (Anaxyrus terrestris)

2016

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“…Larval source management (LSM) is part of integrated vector control using strategies to modify or manipulate water bodies as the potential larval habitats of mosquitoes to prevent maturation of mosquito developmental stages. LSM strategies also include the introduction of larvicide (chemical and biological agents) and larvivorous fish (natural predator) into larval habitats [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have proposed the utility of using metallic [8,9] and liquid [5,10,11] copper (Cu) as a potential and affordable larvicide [7]. Copper is effective as a mosquito larvicide at a concentration below 2 ppm [9], the threshold value deemed safe for human drinking water [12], thus making copper a potential candidate for use in the public health setting.…”

Section: Introductionmentioning

confidence: 99%

Laboratory testing of low concentration (<1 ppm) of copper to prolong mosquito pupation and adult emergence time: An alternative method to delay mosquito life cycle

Reza

Ilmiawati

2020

PLoS ONE

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Introduction Larvicide application in ovitrap is one of the currently available methods used in mosquito eradication campaign. We previously reported that copper in liquid form is a promising candidate due to its potent larvicide properties in a laboratory setting and in the field. In the field study, several larvae survived in outdoor ovitrap due to the dilution of copper concentration by rainwater. The surviving larvae were smaller and less motile. This led our interest to study the effect of a sub-lethal dose of copper in ovitrap on mosquito larval development, pupation time and lifespan in the adult stage. Methods First instar larvae of Aedes albopictus, Anopheles stephensi, and Culex pipiens were put in water containing 0.15 ppm, 0.30 ppm, and 0.60 ppm of copper. The surviving larvae, the emerging pupae, and adult mosquitoes were observed and counted every 24-hour and statistically analyzed by t-test or Mann-Whitney U test. Inter-species difference in response to different concentration of copper were also analyzed. Results Copper showed a potent larvicide effect at 0.60 ppm concentration. Prolonged pupation time and a lower number of adult mosquitoes were observed at 0.15 ppm concentration. Copper exposure did not affect adult mosquitoes' lifespan. Culex pipiens was the most susceptible species to copper exposure. Conclusion This study demonstrates the efficacy of copper at <1 ppm to kill mosquito larvae and to prolong pupation and adult emergence time. Utilization of copper at a low concentration is cost

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Section: Introductionmentioning

confidence: 99%

“…LSM strategies also include the introduction of larvicide (chemical and biological agents) and larvivorous fish (natural predator) into larval habitats [5–7]. Previous studies have proposed the utility of using metallic [8, 9] and liquid [5, 10, 11] copper as a potential and affordable larvicide [7]. Copper is effective as a mosquito larvicide at a concentration below 2 ppm [9], the threshold value deemed safe for human drinking water [12], thus making copper a potential candidate for use in the public health setting.…”

Section: Introductionmentioning

confidence: 99%

Laboratory testing of very low-copper-treated water to prolong pupation and emerging time of mosquito larvae: an alternative method to delay mosquito breeding capability

Reza

Ilmiawati

2019

Preprint

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IntroductionLarvicide application in ovitrap is one of the currently available methods used in mosquito eradication campaign because they eliminate the larval stage. We previously reported that copper in liquid form is a promising candidate due to its potent larvicide properties in a laboratory setting and even in the field. In the field study, several larvae survived in the ovitrap due to the dilution of copper concentration by the rain. The surviving larvae were smaller and less motile. This led our interest to study the effect of a sub-lethal dose of copper in ovitrap on larvae development, pupation time and lifespan in the adult stage.MethodsFirst instar larvae of three species of mosquito (Aedes albopictus, Culex pipiens and Anopheles stephensi) were bred in copper-treated water at a concentration of 0.60 ppm, 0.30 ppm, and 0.15 ppm and compared with the control group. The surviving larvae were recorded every day in terms of pupa emerging time and adult emerging time. The number of adult mortality was recorded and compared with the control.ResultsCopper showed potent larvicide effect in the 0.60 ppm concentration and prolonged pupation time and caused a significantly lower number of emerging mosquitoes down to the lowest concentration of 0.15 ppm. The adult lifespan was not different compared to the control.ConclusionThis study demonstrates the capability of copper below 1 ppm to prolong the pupation time and the emerging time of mosquito larvae. Our findings open the possibility of copper application to cut mosquito breeding capacity that eventually will reduce disease transmission.

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Laboratory testing of larvivorous fish Japanese medaka (Oryzias latipes) predation ability to copper-treated Anopheles stephensi larvae: An alternative method for vector control under low concentration of copper

Cited by 3 publications

References 15 publications

Effects of metal and predator stressors in larval southern toads (Anaxyrus terrestris)

Effects of metal and predator stressors in larval southern toads (Anaxyrus terrestris)

Laboratory testing of low concentration (<1 ppm) of copper to prolong mosquito pupation and adult emergence time: An alternative method to delay mosquito life cycle

Laboratory testing of very low-copper-treated water to prolong pupation and emerging time of mosquito larvae: an alternative method to delay mosquito breeding capability

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