Laboratory tests performed on Leishmania seroreactive dogs euthanized by the leishmaniasis control program

Silva, D.A.; Madeira, M.F.; Teixeira, André Costa; Souza, Carina Margonari de; Figueiredo, Fabiano Borges

doi:10.1016/j.vetpar.2011.01.048

Cited by 46 publications

(33 citation statements)

References 14 publications

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“…For this, was structured a network of immunofluorescence assay (IFA) using eluate of filter paper; all dogs with positive result have been euthanized. However, dogs evaluate by IFA employing eluate when reevaluated by IFA using serum samples, 72% of the animals were negative (Silva et al, 2011). This result can be partially explained by the sample tested, serum and eluate, as previously reported by Figueiredo et al, (2010).…”

Section: Prophylaxis Of Vlsupporting

confidence: 59%

Euthanasia for the Zoonosis Control Program

Laurenti¹,

Moreira²

2011

Euthanasia - The "Good Death" Controversy in Humans and Animals

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Section: Prophylaxis Of Vlsupporting

confidence: 59%

Euthanasia for the Zoonosis Control Program

Laurenti¹,

Moreira²

2011

Euthanasia - The "Good Death" Controversy in Humans and Animals

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“…Skin samples were packed in phosphate buffer saline (PBS) solution, with the addition of antibiotics (penicillin and streptomycin) and an antifungal (fluorocytosine), and the bone marrow samples were stored directly in Novy, MacNeal, Nicole (NNN) biphasic culture, both transported under refrigeration for processing at the Laboratory for Clinical Research and Surveillance in Leishmaniasis (LapClinVigiLeish) at the National Institute of Infectolgy (INI/Fiocruz) for parasitological culture based on the protocol by Silva et al 21 . Isoenzymatic characterization of the isolates followed the protocol by Cupolillo et al 22 .…”

Section: Figurementioning

confidence: 99%

Fatores ambientais associados à ocorrência de leishmaniose visceral canina em uma área de recente introdução da doença no Estado do Rio de Janeiro, Brasil

et al. 2018

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Resumo: Foi avaliada a associação entre características ambientais obtidas por sensoriamento remoto e a prevalência da leishmaniose visceral canina (LVC) no bairro do Jacaré, área de recente introdução da doença, no Município de Niterói, Estado do Rio de Janeiro, Brasil. Trata-se de um estudo seccional para avaliação da prevalência de LVC, definida por meio da positividade no teste imunocromatográfico rápido em dupla plataforma (dual path platform - DPP), confirmada com o ensaio imunoenzimático (EIE). Foram incluídos 97 cães com prevalência de LVC de 21,6%. Houve maior frequência de LVC em cães com a convivência com outro cão, gambá, mico e ouriço-terrestre, assim como com a história de remoção de outros cães com LVC do domicílio. Na análise multivariada, ajustada por sexo e idade do cão, cães residentes em áreas com maior cobertura de vegetação esparsa apresentaram prevalência da infecção por Leishmania infantum cinco vezes maior do que aqueles que residiam em áreas menos vegetadas (OR = 5,72; IC95%: 1,47-22,20). Por outro lado, áreas mais urbanizadas caracterizadas como comerciais ou residenciais carentes, identificadas pelo sensoriamento remoto como aquelas com alta densidade de estruturas cinza, estiveram associadas à menor ocorrência da LVC (OR = 0,09; IC95%: 0,01-0,92). A maior prevalência de infecção em cães convivendo com outros animais silvestres e em áreas com maior cobertura vegetal, associada com menor prevalência em áreas urbanizadas, indica um padrão rural de transmissão da LVC nessa área.

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“…Such a measure, although applied in endemic areas, has been questioned in view of possible crossreactions obtained in serological tests (Coutinho et al 1985, Vexenat et al 1996, mainly in areas of overlapping with other trypanosomatids agents (Madeira et al 2006, Silva et al 2011.…”

Section: Introductionmentioning

confidence: 99%

Use of Parasitological Culture to Detect Leishmania (Leishmania) chagasi in Naturally Infected Dogs

Almeida

Sousa²,

Sorte³

et al. 2011

Vector-Borne and Zoonotic Diseases

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In Brazil, although the domestic dog is a major target for the control actions for visceral leishmaniasis, knowledge gaps of the Leishmania species present in those animals still exist in many endemic areas. The objective of this study was the use of parasitological culture as a diagnosis tool and identification of species of Leishmania and other trypanosomatids in the canine population in the city of Cuiaba/Mato Grosso. Biological samples such as blood, intact skin fragments, cutaneous ulcers, and bone marrow were collected during a cross-sectional study and cultured on biphasic medium (Novy-MacNeil-Nicolle [NNN]/Schneider's). Leishmania isolates were characterized through isoenzyme electrophoresis. Isolates were obtained from 11.2% (n=54) of the 482 animals studied considering the different anatomical sites investigated. Leishmania chagasi was confirmed in 8.3% (n=40) dogs and Trypanosoma caninum in 2.9% (n=14). The sample of intact skin presented a higher chance of isolation of L. chagasi in symptomatic dogs and bone marrow in asymptomatic dogs (p<0.05). The results presented in this study emphasize the value of culture and confirm, for the first time, the circulation of L. chagasi in the canine population in different neighborhoods of the city of Cuiaba and broaden the knowledge of the geographical distribution of T. caninum in Brazil.

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Laboratory tests performed on Leishmania seroreactive dogs euthanized by the leishmaniasis control program

Cited by 46 publications

References 14 publications

Euthanasia for the Zoonosis Control Program

Euthanasia for the Zoonosis Control Program

Fatores ambientais associados à ocorrência de leishmaniose visceral canina em uma área de recente introdução da doença no Estado do Rio de Janeiro, Brasil

Use of Parasitological Culture to Detect Leishmania (Leishmania) chagasi in Naturally Infected Dogs

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