Laboratory trials to infect insects and nematodes by some acaropathogenic Hirsutella strains (Mycota: Clavicipitaceous anamorphs)

Bałazy, Stanisław; Wrzosek, Marta; Sоsnowska, Danuta; Tkaczuk, C.; Muszewska, Anna

doi:10.1016/j.jip.2007.08.006

Cited by 21 publications

(22 citation statements)

References 20 publications

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“…This region is highly conserved and is commonly used as a sole tool or supplemented with other universal sequences (e.g. tubulin and actin) for identification, characterization and phylogenetic analysis of fungal isolates (Balazy et al, 2008). The ITS region has been used in other studies for successful identification of entomopathogenic fungi (Islam et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and evaluation of indigenous entomopathogenic fungal isolates against Sorghum Chafer, Pachnoda interrupta (Olivier) in Ethiopia

Habtegebriel¹,

Getu²,

Dawd³

et al. 2016

J. Entomol. Nematol.

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Sorghum chafer, Pachnoda interrupta (Olivier) is a damaging pest of sorghum, other cereals and horticultural crops in Africa which results in complete crop loss. Currently, the management strategies rely heavily on chemical pesticides, which do not provide effective control. There is evidence showing that microbial biocontrol agents have the potential to control P. interrupta. In this study, the entomopathogenic fungi isolated from soils and insects in Ethiopia were identified using morphological and cultural characteristics. These were then evaluated for viability and virulence against Galleria mellonella (Fabricius) (Lepidoptera, Pyralidae) larvae and P. interrupta adults. Firstly, 116 Metarhizium spp. and Beauveria spp. were considered. The isolates were evaluated based on high viability as measured by percentage germination on SDA media. Only 56 isolates had greater than 70% viability. Secondly, these were further evaluated using conidial suspensions applied to G. mellonella larvae at a concentration of 1×10 8 conidia/ml. Four Beauveria spp. and three Metarhizium spp. isolates which caused over 60% mortality were selected for final virulence assay against adults of P. interrupta. The selected isolates were further confirmed by PCR amplification of ITS4 and ITS5 gene regions and chi1 and chi4 primers. Finally, the seven isolates were evaluated for virulence against P. interrupta at a dose of 1 mg dry conidia/10 beetles under laboratory condition. Mortality of P. interrupta ranged from 14% for isolate 9604 to 82% for isolate PPRC51. Dose-response tests showed that the LD 50 of PPRC2 (0.62 mg/10 beetles) and PPRC51 (0.55 mg/10 beetles) isolates were not significantly different from each other. The results demonstrated the high potential of the two isolates (PPRC51 and PPRC2) as microbial biocontrol agents. However, field evaluation of the isolates should be performed for their development into a mycopesticide against P. interrupta.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization and evaluation of indigenous entomopathogenic fungal isolates against Sorghum Chafer, Pachnoda interrupta (Olivier) in Ethiopia

Habtegebriel¹,

Getu²,

Dawd³

et al. 2016

J. Entomol. Nematol.

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“…Fungi were isolated by common insect pathology techniques (Bałazy et al 2008). The standard potatodextrose agar (PDA), Sabouraud-dextrose agar (SDA) and SDA enriched with egg-yolk (SDEYA) media were used, the latter sometimes supplemented with powdered milk and/or antibiotics.…”

Section: Methodsmentioning

confidence: 99%

“…Most material included here has been collected or identified in [2003][2004][2005][2006][2007], and this paper is supplementary to three previous publications Bałazy et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Diversity of acaropathogenic fungi in Poland and other European countries

Bałazy¹,

Miętkiewski

Tkaczuk

et al. 2008

Exp Appl Acarol

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The occurrence, species diversity and some aspects of taxonomical affinity and host selectivity of acaropathogenic fungi associated with phytophagous, saprotrophic and predacious mites in Poland and other European countries were investigated on wild and cultivated plants, in insect feeding sites under the bark and in decayed wood. From among 33 species of fungi affecting mites only five species of Entomophthorales were separated and the most numerous were Neozygites floridana mostly on Tetranychus urticae, N. abacaridis on a few eriophyid species, and Conidiobolus coronatus attacking gamasid mites most frequently of the genus Dendrolaelaps. The most frequent mite pathogens occurring in mite communities on plants and in wood infested by insects were of the genus Hirsutella. Until now 13 of their form-species have been recognized in these habitats, but only H. kirchneri, H. necatrix and H. thompsonii (including its variety synnematosa) can be treated as exclusive oligophagous pathogens of phytophagous mites, though their potential host range seems to embrace only selected eriophyid or tarsonemid mites. Taxonomical differentiation of fungal strains was based on close morphological observations and molecular analysis of ITS region sequences. Two new species of acaropathogenic fungi were described in these studies. Hirsutella danubiensis sp. nov. was found in the tetranychid T. urticae, whereas H. vandergeesti sp. nov. affected phytoseiid mites of the genera Amblyseius, Neoseiulus, Seiulus and Typhlodromus, and the tarsonemid Tarsonemus lacustris.

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“…ITS is a conserved rDNA sequence that has been widely used both alone and in combination with other universal sequences, such as β-tubulin, actin, etc., to identify, characterize, and to perform phylogenetic analysis of fungal isolates (Balazy et al, 2008). The sequence length of ITS region was found to be 500-650bp (Approx.)…”

Section: Molecular Identification Of Chaetomium Isolates Based On Itsmentioning

confidence: 99%

Studies on Phylogeny of Chaetomium Species of India

Sekhar¹,

Prameeladevi²,

Kamil³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Laboratory trials to infect insects and nematodes by some acaropathogenic Hirsutella strains (Mycota: Clavicipitaceous anamorphs)

Cited by 21 publications

References 20 publications

Molecular characterization and evaluation of indigenous entomopathogenic fungal isolates against Sorghum Chafer, Pachnoda interrupta (Olivier) in Ethiopia

Molecular characterization and evaluation of indigenous entomopathogenic fungal isolates against Sorghum Chafer, Pachnoda interrupta (Olivier) in Ethiopia

Diversity of acaropathogenic fungi in Poland and other European countries

Studies on Phylogeny of Chaetomium Species of India

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