Laccase-conjugated thiolated chitosan-Fe3O4 hybrid composite for biocatalytic degradation of organic dyes

Ulu, Ahmet; Birhanlı, Emre; Boran, Filiz; Köytepe, Süleyman; Yeşilada, Özfer; Ateş, Burhan

doi:10.1016/j.ijbiomac.2020.02.006

Cited by 62 publications

(36 citation statements)

References 64 publications

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“…However, in the spectra of HNTs-Fe 3 O 4 -CTs-SH, the S-H stretch in the region of 2600-2550 cm −1 was absent. A similar observation for the absence of S-H stretch in the region of 2600-2550 cm −1 for thiolated chitosan was observed by an earlier report [7]. This might be due to selective thiolation rather than thiolation of most of the -NH 2 group of the chitosan.…”

Section: Xrd Ft-ir Vsm and Xps Analysissupporting

confidence: 89%

“…However, the additional peaks of 1659, 1255, and 623 cm −1 were observed in the HNTs-Fe 3 O 4 -CTs-SH. These peaks mainly corresponded to the C=O stretching amides, C-SH stretching, and C-S stretching, respectively [7,25]. The appearance of these peaks successfully corroborated the thiolation of the chitosan.…”

Section: Xrd Ft-ir Vsm and Xps Analysismentioning

confidence: 63%

“…The thiol group from the nanocomposite reacted with the thiol group from the laccase to form the strong disulfide (-S-S-) bond for immobilization. The thiol group displayed excellent immobilization performance for the enzyme laccase [7]. The laccase immobilized HNTs-Fe3O4-CTs-SH is denoted as HNTs-Fe3O4-CTs-S-S-Laccase ( Figure 1C).…”

Section: Synthesismentioning

confidence: 99%

“…Various methods and combinations of treatment were employed to face this challenge [6]. However, enzymatic methods unveiled a new outlook to treat waste streams to get rid of intractable organic pollutants [7]. Laccase is one of the most important enzymes in terms of waste pollutant removal.…”

Section: Introductionmentioning

confidence: 99%

“…Thiolation of the CTs involves modification of the surface amino (-NH 2 ) group to the thiol (-SH) group by NHS-ester reaction [22]. The enzymes possess the -SH group due to the presence of amino acids such as cysteine and methionine [7]. The -SH group from the thiolated supports and enzyme react at pH 5 to form a very stable (-S-S-) disulfide bond for immobilization [23].…”

Section: Introductionmentioning

confidence: 99%

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Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

et al. 2020

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This study focuses on the development of a nanosupport based on halloysite nanotubes (HNTs), Fe3O4 nanoparticles (NPs), and thiolated chitosan (CTs) for laccase immobilization. First, HNTs were modified with Fe3O4 NPs (HNTs-Fe3O4) by the coprecipitation method. Then, the HNTs-Fe3O4 surface was tuned with the CTs (HNTs-Fe3O4-CTs) by a simple refluxing method. Finally, the HNTs- Fe3O4-CTs surface was thiolated (-SH) (denoted as; HNTs- Fe3O4-CTs-SH) by using the reactive NHS-ester reaction. The thiol-modified HNTs (HNTs- Fe3O4-CTs-SH) were characterized by FE-SEM, HR-TEM, XPS, XRD, FT-IR, and VSM analyses. The HNTs-Fe3O4-CTs-SH was applied for the laccase immobilization. It gave excellent immobilization of laccase with 100% activity recovery and 144 mg/g laccase loading capacity. The immobilized laccase on HNTs-Fe3O4-CTs-SH (HNTs-Fe3O4-CTs-S-S-Laccase) exhibited enhanced biocatalytic performance with improved thermal, storage, and pH stabilities. HNTs-Fe3O4-CTs-S-S-Laccase gave outstanding repeated cycle capability, at the end of the 15th cycle, it kept 61% of the laccase activity. Furthermore, HNTs-Fe3O4-CTs-S-S-Laccase was applied for redox-mediated removal of textile dye DR80 and pharmaceutical compound ampicillin. The obtained result marked the potential of the HNTs-Fe3O4-CTs-S-S-Laccase for the removal of hazardous pollutants. This nanosupport is based on clay mineral HNTs, made from low-cost biopolymer CTs, super-magnetic in nature, and can be applied in laccase-based decontamination of environmental pollutants. This study also gave excellent material HNTs-Fe3O4-CTs-SH for other enzyme immobilization processes.

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Section: Xrd Ft-ir Vsm and Xps Analysissupporting

confidence: 89%

Section: Xrd Ft-ir Vsm and Xps Analysismentioning

confidence: 63%

Section: Synthesismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

et al. 2020

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Biomedical Applications of Nano‐Engineered Enzymes: Current Status and Future Direction

Safary¹,

Akbarzadeh²,

Barar³

et al. 2021

Nanoengineering of Biomaterials

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Functionalized polyacrylonitrile particles as a promising support for the immobilization of laccase fromTrametes versicolor

Vieira

Henriques

Gurgel

et al. 2023

J of Applied Polymer Sci

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This work evaluates the potential of polyacrylonitrile particles (PAN) as support for laccase from Trametes versicolor immobilization. The slurry polymerization method forms mesoporous particles with low surface area and low maximum pore volume in desorption. The particles were chemically modified by consecutive alkaline and acid hydrolysis, followed by amination and activation with glutaraldehyde to enzyme immobilization. The laccase immobilization yield was 99.48% and 14.29% using the functionalized and non-functionalized particles, respectively. The enzyme activity was measured by the oxidation of 2,2 0 -azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) at different pHs and temperatures. The PAN/laccase derivative was hyperactivated at pH 3, up to 3 times higher than the free enzyme, and performed better at 50 C after 6 h of incubation, with relative activity up to 33% higher than the free enzyme. However, both enzymes denatured when the conditions reached pH 8 and 70 C. The PAN/laccase retained 89% of the initial activity after 30 days of storage at 5 C. It was possible to reuse the enzymatic derivative for 5 cycles, with up to 50% residual activity, under 50 C and pH 3. These results show the potential of this new support for laccase immobilization and further applications of industrial interest.

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Laccase-conjugated thiolated chitosan-Fe3O4 hybrid composite for biocatalytic degradation of organic dyes

Cited by 62 publications

References 64 publications

Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

Thiolation of Chitosan Loaded over Super-Magnetic Halloysite Nanotubes for Enhanced Laccase Immobilization

Biomedical Applications of Nano‐Engineered Enzymes: Current Status and Future Direction

Functionalized polyacrylonitrile particles as a promising support for the immobilization of laccase fromTrametes versicolor

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