Laccase immobilized on Au confined MXene based electrode for electrochemical detection of catechol

Applications and immobilization strategies of the copper-centred laccase enzyme; a review

“…It is usually used in biosensors and biofuel cells to provide a high adsorption for laccase and to prevent enzyme leaching from the electrode surface as well as undesirable side reactions [ 567 , 568 , 569 ]. Nafion is preferred in biosensors because its negatively charged polyelectrolyte matrix eliminates the influence of interfering signals hence improving the sensitivity of the sensor [ 323 ]. For example, Litescu et al [ 327 ], drop-casted a laccase solution on a screen printed electrode, allowed it to dry with forced air followed by immobilization of a Nafion membrane to protect the enzyme from leaching.…”

2D MXene‐Based Biosensing: A Review

“…Chandran et al. [ 309 ] developed a biosensor‐based Laccase (Lac) immobilized to the Au/MXene/GCE using Nafion to detect catechol. The prepared composite facilitated electron exchange and exhibited amperometric response in the range (0.05 to 0.15 n m ) with a detection limit (0.05 m m ).…”

Immobilization of laccase on Fe3O4@MF-CNTs for the rapid and sensitive biosensing of catechol

“…4–6 Among these compounds, catechol is considered to be the most dangerous as it can damage the central nervous system and inhibit DNA replication. 7–9 Because catechol is water-soluble, it is easy to permeate and migrate in the environment, which makes it widely exist in natural water sources and thus pose a threat to human health. 4,7 Therefore, various detection techniques, including chromatography and electrochemical biosensing, 10–13 have been developed for the treatment of catechol to avoid catechol pollution.…”

“…7–9 Because catechol is water-soluble, it is easy to permeate and migrate in the environment, which makes it widely exist in natural water sources and thus pose a threat to human health. 4,7 Therefore, various detection techniques, including chromatography and electrochemical biosensing, 10–13 have been developed for the treatment of catechol to avoid catechol pollution. Among these techniques, enzyme based electrochemical biosensing is one of the most promising and widely used methods owing to its high selectivity, fast response, convenience and low cost.…”