Lachnospiraceae shift in the microbial community of mice faecal sample effects on water immersion restraint stress

Li, Shiyin; Wang, Zelin; Yang, Yun; Yang, Sha; Yao, Chenchen; Liu, Kaiyun; Cui, Sixin; Zou, Quanming; Sun, Hongwu; Guo, Gang

doi:10.1186/s13568-017-0383-4

Cited by 43 publications

(36 citation statements)

References 18 publications

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“…The relative abundance of Lachnoclostridium was also found decreased in children with ASD [63] and in ADHD patients compared to controls [34,35]. Going up to the phylum level, the differentially abundant genera in our study belonged predominantly (24 out of 31) to the phylum Firmicutes, and families Lachnospiraceae and Ruminococcaceae, which have been associated with stress and social avoidance behavior [64,65]. Ten genera of the Lachnospiraceae family were more abundant in mice ADHD compared to mice control ; in the latter group, three other Lachnospiraceae genera were enriched.…”

Section: Discussionsupporting

confidence: 50%

Gut microbiota from persons with attention-deficit/hyperactivity disorder affects the brain in mice

Tengeler¹,

Dam

Wiesmann³

et al. 2020

Microbiome

105

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Background: The impact of the gut microbiota on host physiology and behavior has been relatively well established. Whether changes in microbial composition affect brain structure and function is largely elusive, however. This is important as altered brain structure and function have been implicated in various neurodevelopmental disorders, like attention-deficit/hyperactivity disorder (ADHD). We hypothesized that gut microbiota of persons with and without ADHD, when transplanted into mice, would differentially modify brain function and/or structure. We investigated this by colonizing young, male, germ-free C57BL/6JOlaHsd mice with microbiota from individuals with and without ADHD. We generated and analyzed microbiome data, assessed brain structure and function by magnetic resonance imaging (MRI), and studied mouse behavior in a behavioral test battery.Results: Principal coordinate analysis showed a clear separation of fecal microbiota of mice colonized with ADHD and control microbiota. With diffusion tensor imaging, we observed a decreased structural integrity of both white and gray matter regions (i.e., internal capsule, hippocampus) in mice that were colonized with ADHD microbiota. We also found significant correlations between white matter integrity and the differentially expressed microbiota. Mice colonized with ADHD microbiota additionally showed decreased resting-state functional MRI-based connectivity between right motor and right visual cortices. These regions, as well as the hippocampus and internal capsule, have previously been reported to be altered in several neurodevelopmental disorders. Furthermore, we also show that mice colonized with ADHD microbiota were more anxious in the open-field test. Conclusions: Taken together, we demonstrate that altered microbial composition could be a driver of altered brain structure and function and concomitant changes in the animals' behavior. These findings may help to understand the mechanisms through which the gut microbiota contributes to the pathobiology of neurodevelopmental disorders.

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Section: Discussionsupporting

confidence: 50%

Gut microbiota from persons with attention-deficit/hyperactivity disorder affects the brain in mice

Tengeler¹,

Dam

Wiesmann³

et al. 2020

Microbiome

105

View full text Add to dashboard Cite

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“…The relative abundance of Lachnospiraceae was significantly reduced in animals fed with MFGM compared to control diet. Increases in this bacteria have been previously noted in an animal model for chronic stress (Li et al, 2017). Lachnospiraceae has an impact on lifespan and the immune system (Maldonado-Contreras et al, 2011) and is associated with diseases such as ulcerative colitis, Crohn's and coeliac.…”

Section: Discussionmentioning

confidence: 85%

The enduring effects of early‐life stress on the microbiota–gut–brain axis are buffered by dietary supplementation with milk fat globule membrane and a prebiotic blend

O’Mahony

Neufeld

Waworuntu

et al. 2019

Eur J of Neuroscience

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Nutritional interventions targeting the microbiota–gut–brain axis are proposed to modulate stress‐induced dysfunction of physiological processes and brain development. Maternal separation (MS) in rats induces long‐term alterations to behaviour, pain responses, gut microbiome and brain neurochemistry. In this study, the effects of dietary interventions (milk fat globule membrane [MFGM] and a polydextrose/galacto‐oligosaccharide prebiotic blend) were evaluated. Diets were provided from postnatal day 21 to both non‐separated and MS offspring. Spatial memory, visceral sensitivity and stress reactivity were assessed in adulthood. Gene transcripts associated with cognition and stress and the caecal microbiota composition were analysed. MS‐induced visceral hypersensitivity was ameliorated by MFGM and to greater extent with the combination of MFGM and prebiotic blend. Furthermore, spatial learning and memory were improved by prebiotics and MFGM alone and with the combination. The prebiotic blend and the combination of the prebiotics and MFGM appeared to facilitate return to baseline with regard to HPA axis response to the restraint stress, which can be beneficial in times where coping mechanisms to stressful events are required. Interestingly, the combination of MFGM and prebiotic reduced the long‐term impact of MS on a marker of myelination in the prefrontal cortex. MS affected the microbiota at family level only, while MFGM, the prebiotic blend and the combination influenced abundance at family and genus level as well as influencing beta‐diversity levels. In conclusion, intervention with MFGM and prebiotic blend significantly impacted the composition of the microbiota as well as ameliorating some of the long‐term effects of early‐life stress.

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“…13 The relative abundance of Lachnospiraceae was elevated in cases, and interestingly, an increase in its population has been demonstrated in autoimmune diseases such as ankylosing spondylitis 16 or primary sclerosing cholangitis. 18 This finding could be further explored in humans due to the relationship between alopecia areata flares and stress. 18 This finding could be further explored in humans due to the relationship between alopecia areata flares and stress.…”

Section: Discussionmentioning

confidence: 97%

“…17 In addition, in an experimental study in a murine model, a significant elevation of the proportion of gut Lachnospiraceae was observed after exposing the animals to great stress. 18 This finding could be further explored in humans due to the relationship between alopecia areata flares and stress. 1 The role of the Clostridiales vadin BB60 group in human disease is currently unknown.…”

Section: Discussionmentioning

confidence: 97%

Analysis of the gut microbiota in alopecia areata: identification of bacterial biomarkers

Moreno‐Arrones

Serrano‐Villar²,

Pérez‐Brocal

et al. 2019

Acad Dermatol Venereol

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Background Alopecia areata is a T‐cell‐mediated autoimmune disease with an unknown etiopathogenesis. Gut microbiota has been revealed as a key modulator of systemic immunity. Objective To determine whether patients affected by alopecia universalis present differences in gut bacteria composition compared with healthy controls and investigate possible bacterial biomarkers of the disease. Methods We conducted a cross‐sectional study that involved 15 patients affected by alopecia universalis and 15 controls. Gut microbiome of the study subjects was analysed by sequencing the 16SrRNA of stool samples. We searched for bacterial biomarkers of alopecia universalis using the linear discriminant analysis effect size (LEFse) tool. Results In total, 30 study subjects (46.6% female; mean [SD] age, 40.1 [9.8] years) were enrolled. Neither alpha (Shannon diversity index 5.31 ± 0.43 vs. 5.03 ± 0.43, P 0.1) or beta diversity (ADONIS P value: 0.35) of gut microbiota showed statistically significant differences between cases and controls. In patients affected with alopecia, we found an enriched presence (LDA SCORE > 2) of Holdemania filiformis, Erysipelotrichacea, Lachnospiraceae, Parabacteroides johnsonii, Clostridiales vadin BB60 group, Bacteroides eggerthii and Parabacteroides distasonis. A predictive model based on the number of bacterial counts of Parabacteroides distasonis and Clostridiales vadin BB60 group correctly predicted disease status in 80% of patients (AUC 0.804 (0.633–0.976), P 0.004). Conclusion Alopecia universalis does not seem to affect broadly gut microbiota structure. Bacterial biomarkers found associated with the disease (Holdemania filiformis, Erysipelotrichacea, Lachnospiraceae, Parabacteroides johnsonii, Eggerthellaceae, Clostridiales vadin BB60 group, Bacteroides eggerthii and Parabacteroides distasonis) should be further studied as they could be involved in its pathophysiology or be used as diagnostic tools.

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Lachnospiraceae shift in the microbial community of mice faecal sample effects on water immersion restraint stress

Cited by 43 publications

References 18 publications

Gut microbiota from persons with attention-deficit/hyperactivity disorder affects the brain in mice

Gut microbiota from persons with attention-deficit/hyperactivity disorder affects the brain in mice

The enduring effects of early‐life stress on the microbiota–gut–brain axis are buffered by dietary supplementation with milk fat globule membrane and a prebiotic blend

Analysis of the gut microbiota in alopecia areata: identification of bacterial biomarkers

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