Lack of accuracy of the noninvasive Helicobacter pylori stool antigen test in patients with gastroduodenal ulcer bleeding

Leerdam, Monique E. van; Ende, Arie van der; Kate, F. J. W. ten; Rauws, E. A. J.; Tytgat, G. N. J.

doi:10.1111/j.1572-0241.2003.07387.x

Cited by 29 publications

(44 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…While some authors report a prevalence of over 90% (19), other authors have described a lower prevalence, between 20 and 70% (17,30). These discrepancies may be due to the different diagnostic methods used, since the techniques employed to diagnose this infection in this type of patients have often been reported to be less sensitive (17,18,32).…”

Section: Discussionmentioning

confidence: 97%

Real-Time PCR for Diagnosing Helicobacter pylori Infection in Patients with Upper Gastrointestinal Bleeding: Comparison with Other Classical Diagnostic Methods

et al. 2012

View full text Add to dashboard Cite

The aim of this study was to determine the diagnostic usefulness of quantification of the H. pylori genome in detection of infection in patients with upper gastrointestinal bleeding (UGB). A total of 158 consecutive patients with digestive disorders, 80 of whom had clinical presentation of UGB, were studied. The number of microorganisms was quantified using a real-time PCR system which amplifies the urease gene with an internal control for eliminating the false negatives. A biopsy sample from the antrum and corpus of each patient was processed. The rapid urease test, culture, histological study, stool antigen test, and breath test were done. The gold standard was a positive culture or positive results in at least two of the other techniques. When a positive result was defined as any number of microorganisms/human cell, the sensitivity of real-time PCR was greater in bleeding patients, especially in the gastric corpus: 68.4% (95% confidence interval [CI], 52.3 to 84.5%) in non-UGB patients versus 91.5% (95% CI, 79.6 to 97.6%) in UGB patients. When a positive result was defined as a number of microorganisms/human cell above the optimal value that maximizes the Youden index (>3.56 microorganisms/human cell in the antrum and >2.69 in the corpus), the sensitivity and specificity in UGB patients were over 80% in both antrum and corpus. Our findings suggest that some bleeding patients with infection caused by H. pylori may not be correctly diagnosed by classical methods, and such patients could benefit from the improved diagnosis provided by real-time PCR. However, the clinical significance of a small number of microorganisms in patients with negative results in classical tests should be evaluated.

show abstract

Section: Discussionmentioning

confidence: 97%

Real-Time PCR for Diagnosing Helicobacter pylori Infection in Patients with Upper Gastrointestinal Bleeding: Comparison with Other Classical Diagnostic Methods

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The first On the other hand, it has been postulated that H. pylori stool antigen tests in patients with gastroduodenal ulcer bleeding are inaccurate, with low sensitivity in some evaluations, 32 and low specificity in other cases. 33,34 Recently, Gisbert et al 35 described…”

Section: Discussionmentioning

confidence: 99%

Comparison of a monoclonal with a polyclonal antibody‐based enzyme immunoassay stool test in diagnosing Helicobacter pylori infection before and after eradication therapy

Domínguez

Forné

Blanco

et al. 2006

Aliment Pharmacol Ther

View full text Add to dashboard Cite

show abstract

“…It is an accurate test in all age groups of children too [19] . However, Peitz et al reported that the diagnostic accuracy, in particular the sensitivity, of the HpSA stool test was reduced by upper gastrointestinal bleeding, and a negative test result should be confirmed by a further diagnostic method [20,21] . Although the HpSA test seems to be less accurate than the UBT, both UBT and stool antigen tests are reliable noninvasive tests for monitoring the efficacy of H pylori eradication therapy.…”

Section: Discussionmentioning

confidence: 99%

Clinical value ofHelicobacter pyloristool antigen test, ImmunoCard STAT HpSA, for detectingH pyloriinfection

Li¹,

Guo²,

Zhang³

et al. 2004

WJG

View full text Add to dashboard Cite

Lack of accuracy of the noninvasive Helicobacter pylori stool antigen test in patients with gastroduodenal ulcer bleeding

Abstract: The HpSA test gave a high number of false- positive results in patients with PUB, probably because of blood constituents cross-reacting in the enzyme immunoassay. The HpSA test is not accurate for testing H. pylori infection in patients with PUB.

Cited by 29 publications

References 22 publications

Real-Time PCR for Diagnosing Helicobacter pylori Infection in Patients with Upper Gastrointestinal Bleeding: Comparison with Other Classical Diagnostic Methods

Real-Time PCR for Diagnosing Helicobacter pylori Infection in Patients with Upper Gastrointestinal Bleeding: Comparison with Other Classical Diagnostic Methods

Comparison of a monoclonal with a polyclonal antibody‐based enzyme immunoassay stool test in diagnosing Helicobacter pylori infection before and after eradication therapy

Clinical value ofHelicobacter pyloristool antigen test, ImmunoCard STAT HpSA, for detectingH pyloriinfection

Contact Info

Product

Resources

About