Lack of association between CGRP-related gene polymorphisms and medication overuse headache in migraine patients

Abstract

We investigated whether calcitonin gene-related peptide (CGRP)-related gene polymorphisms are involved in the aggravation of migraines due to medication overuse. In total, 47 migraine patients (6 males and 41 females; 36.4 ± 10.3 years) and 22 medication overuse headache (MOH) patients (1 male and 21 females; 39.6 ± 9.9 years) who had migraine participated in this study. Calcitonin gene-related polypeptide-alpha (CALCA, α-CGRP, Insertion/Deletion rs1553005, rs145837941) and CGRP receptor (receptor activity-modifying protein 1: RAMP1, rs3754701, rs7590387) were analyzed by polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (PCR-RFLP) methods. No significant differences were observed in the genotype distributions of CALCA and RAMP1 between migraine patients and MOH patients. The results of this study showed no association between CGRP-related gene polymorphisms and the complication of MOH in migraine patients.

Calcitonin-Related Polypeptide Alpha Gene Polymorphisms and Related Diseases

“…Primer sequences are used to determine CALCA-1750 (C/G) gene polymorphism; forward primer: 5 0 -TAG CTG GTA TTT CCC ACA GAG-3 0 and reverse primer: 5 0 -CCC ATT TCA AAG ATG AGT ACC CTG-3. The genotype distributions of CALCA-1750 (C/G) gene polymorphisms are determined by using Bsu36I restriction enzyme and product length is observed: 167 bp for the GG genotype; 167, 142, 25 bp for the CG genotype; 142, 25 bp for the CC genotype [3].…”

“…The most common of the CALCA gene polymorphisms is the CALCA T692C gene polymorphism. Besides this polymorphism, there are also various polymorphisms of CALCA gene such as CALCA-1786T>C, CALCA-624 (T/C), and CALCA (I/D) [2,3].…”