Lack of association of IL8 gene polymorphisms with familial vesico-ureteral reflux

Abstract

Vesico-ureteral reflux (VUR) is the most common inherited disorder of the lower urinary tract. Children with VUR are at risk for ongoing renal damage with subsequent infections. IL8 is an important inflammatory mediator which can be produced by epithelial cells of the renal tract in response to a variety of inflammatory stimuli. High serum concentrations of IL-8 have been reported in patients with chronic renal failure. Elevated IL-8 levels have been reported in the urine of patients with VUR and renal parenchymal scarring (RPS). More recently it was reported that urine IL-8 levels remain elevated in infants with VUR even in the absence of a urinary tract infection (UTI). Increased IL-8 expression has been shown to be associated with polymorphism at position -251 (rs4073) of the IL-8 promoter. The aim of this study was to examine the association of IL-8 gene polymorphism with familial VUR in a cohort of 219 siblings from 109 families affected with VUR, the largest such cohort tested to date. RPS was assessed using dimercaptosuccinic acid scintigraphy. Genotyping was performed in 219 siblings with VUR (157 without RPS, 62 with RPS) and 292 controls for the position -251 of IL-8 gene by polymerase chain reaction with tetra primers and gel analysis. Genotype was compared using the chi square test. Statistical significance was taken as a value of P < 0.05. There were no significant differences in IL-8 -251 genotype frequency between VUR patients and controls. Similarly, gender, severity of VUR and renal parenchymal scarring had no effect on IL-8 -251 genotype frequency. Although IL-8 urinary levels have been reported to be elevated in VUR, our data indicate that IL-8 gene is not involved in the pathogenesis of familial VUR or reflux nephropathy.

A case-control study of interleukin-12 1188A/C polymorphism in obsessive-compulsive disorder in Chinese population

“…It can then participate in the progress of anti-infection and inflammatory disease. With regards directly to OCD, recent studies indicated that cytoactive material such as inflammatory factors may participate in the aetiology of OCD (24). In addition, it is of interest that serum levels of IL-12 were found to be increased in a group of 14 patients with acute Sydenham's chorea compared with control serum (8).…”

“…Its association with other mental disorders such as TS was also been reported (7). With regards directly to OCD, recent studies indicated that cytoactive material such as inflammatory factors may participate in the aetiology of OCD (24). Given that IL-12 is the strongest cytoactive material stimulating cellular immunity to date, IL-12 is worth to be investigated as a candidate gene, which might be related to OCD.…”

Genetic susceptibility to renal scar formation after urinary tract infection: a systematic review and meta-analysis of candidate gene polymorphisms

“…In detail, four studies did not describe the ethnicity of the participating population of the study [38,41,48,54]. Five studies were prospective [32,38,42,44,50], and the other studies did not specify the design, but based on their description in the "method" section, these latter studies can be considered to be retrospective.…”

“…Based on the criteria for assessing the quality of the studies (Table 1), 11 studies (61%) were identified as being of a poor quality design [32,38,41,44,45,[47][48][49][52][53][54].…”

Association Study between IL-1β−511 C/T Polymorphism and Obsessive-Compulsive Disorder (OCD) in Chinese Han Population

“…Genomic DNA was extracted from peripheral blood leukocytes using standard methods. The polymorphisms at -511 C/T in the promoter region of IL-1b were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) according to the procedure described by Kuroda et al [22]. For all data of patients and controls, the Hardy-Weinberg equilibrium of the genotype distribution was tested using the homogeneity chi-square test.…”