Lack of correlation between Bartonella DNA detection within fleas, serological results, and results of blood culture in a Bartonella-infected stray cat population

Scola, Bernard La; Davoust, Bernard; Boni, Mickaël; Raoult, Didier

doi:10.1046/j.1469-0691.2002.00434.x

Cited by 48 publications

(53 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…First, non-infected rodents hosted infected fleas, and infected rodents carried negative fleas. This lack of correlation between infected and non-infected individuals was previously observed (La Scola et al, 2002;Brinkerhoff et al, 2010;Billeter et al, 2011). Second, when the Bartonella infection at a genotype level was analyzed, a clear trend of some genotypes to dominate a particular niche was revealed.…”

Section: Discussionmentioning

confidence: 51%

The effect of ecological and temporal factors on the composition of Bartonella infection in rodents and their fleas

et al. 2014

View full text Add to dashboard Cite

The composition of Bartonella infection was explored in wild Gerbillus andersoni rodents and their Synosternus cleopatrae fleas. Rodent blood samples and fleas were collected in two periods (two different seasons; 4 months apart) from juveniles and adult hosts, and their bartonellae lineages were identified by a 454-pyrosequencing analysis targeting a specific Bartonella citrate synthase gene (gltA) fragment. The rate of Bartonella spp. co-infection was estimated and the assemblage and distribution of bartonellae lineages across the samples with respect to ecological and phylogenetic distance similarities were analyzed. Moreover, environmental factors that could explain potential differences between samples were investigated. Out of the 91 bartonellae-positive samples, 89% were found to be co-infected with more than two phylogenetically distant Bartonella genotypes and additional closely related (but distinguishable) variants. These bartonellae lineages were distributed in a non-random manner, and a negative interaction between lineages was discovered. Interestingly, the overall composition of those infections greatly varied among samples. This variability was partially explained by factors, such as type of sample (blood versus fleas), flea sex and period of collection. This investigation sheds light on the patterns of Bartonella infection and the organization of Bartonella lineages in fleas and rodents in nature.

show abstract

Section: Discussionmentioning

confidence: 51%

The effect of ecological and temporal factors on the composition of Bartonella infection in rodents and their fleas

et al. 2014

View full text Add to dashboard Cite

show abstract

“…In humans, B. henselae seroprevalence ranges between 5.7% in healthy human blood donors [52] and up to 87% in human patients with suspected cat scratch disease [57]. Cats appear to be the main reservoir of B. henselae infection as indicated by the high seroprevalences found in cats worldwide [7] as well as documentation of persistent bacteremia in naturally and experimentally infected cats [1,40,43]. Bacteremia has been infrequently documented in dogs, and only by PCR amplification [6,51].…”

Section: Discussionmentioning

confidence: 99%

Bartonella henselae IgG antibodies are prevalent in dogs from southeastern USA

et al. 2004

View full text Add to dashboard Cite

-In contrast to the large body of literature regarding Bartonella henselae in humans and cats, there is little information about B. henselae as an infectious agent in dogs. Due to the paucity of information regarding the B. henselae serology in dogs, we performed a cross-sectional serosurvey using B. henselae antigen in order to compare the seroprevalence between sick and healthy dogs from the south-eastern USA. Ninety-nine sera were collected from clinically healthy dogs. Three hundred and one sera from sick dogs were submitted to North Carolina State University for serologic screening against a panel of arthropod-transmitted organisms. Serological tests were performed using B. henselae (Bh), Rickettsia rickettsii (Rr), Ehrlichia canis (Ec), Bartonella vinsonii subspecies berkhoffii (Bvb), Babesia canis (Bc) and Borrelia burgdorferi (Bb) antigens. Serum B. henselae IgG antibodies were detected in 10.1% of healthy dogs and in 27.2% of sick dogs. The difference in seroprevalence between the two groups was statistically significant. The majority of seroreactive dogs (80%) had low titers of 1:64 or 1:128. In healthy dogs, seroprevalence for Rr was 14.1% and for Bvb was 1%. In sick dogs, Rr seroprevalence was 29.7%, Ec 6.5%, Bvb 4.7%, Bb 1.7% and Bc was 0.85%. Of the sick dogs that were seroreactive to B. henselae antigens, 40.6% were also seroreactive to Rr, 15.0% reactive to Bvb antigens, 14.8% reactive to Ec antigens, 1.8% reactive to Bc antigens and 1.75% reactive to Bb antigens. Sera from dogs experimentally infected with B. vinsonii subsp. berkhoffii, E. canis or R. rickettsii did not cross react with B. henselae antigens, by IFA testing. This study indicates that B. henselae IgG antibodies are prevalent in healthy and sick dogs living in the south-eastern USA. Nevertheless, further studies are needed to evaluate the epidemiological, clinical and zoonotic relevance of B. henselae infection in dogs.Bartonella henselae / dog / serology / vector-borne diseases

show abstract

“…Therefore, it would be reasonable to hypothesize that antibodies against the antigens of B. henselae type II might not reacted with the antigens of B. henselae type I. Previous reports [11,21] have also shown that cats with B. henselae type II or B. clarridgeiae bacteremia could Sero-negative but PCR-positive 2 12…”

mentioning

confidence: 99%

Cat-scratch disease in veterinary-associated populations and in its cat reservoir in Taiwan

Chang

Lee²,

Maruyama³

et al. 2006

Vet. Res.

View full text Add to dashboard Cite

-In Taiwan, the first human case of cat-scratch disease (CSD) was diagnosed by a serologic test in 1998. Since then, no studies have been conducted to understand the epidemiology of the infection in Taiwan. Therefore, this study is the first epidemiologic survey of CSD in cats and humans in this country. Using veterinary-associated individuals as the study population, it was identified that 1.7% of them were seropositive for B. henselae, and residence was the only factor associated with seropositivity. Bartonella species were successfully isolated from 25 (19.1%) of the 131 cats tested. Only B. henselae and B. clarridgeiae were obtained from bacteremic cats. Furthermore, 9.2% of 131 cats were dually-infected with genotypes I and II of B. henselae. It is the highest prevalence of co-infection that has ever been reported worldwide. In cats, the seroprevalence was 23.7% by indirect immunofluorescence antibody assay with B. henselae Houston-1 (type I) as the antigen. When 12 bacteremic but seronegative cats were re-tested by IFA slides coated with B. henselae U-4 antigen (type II), 9 cats were identified to be seropositive. Our study further suggested that using only direct PCR of 16S-23S rRNA intergenic region or the combination of the PCR method and indirect immuno-fluorescence test will be useful to diagnose Bartonella-free cats.Bartonella / cat scratch disease / cat / veterinary-associated population / Taiwan

show abstract

Lack of correlation between Bartonella DNA detection within fleas, serological results, and results of blood culture in a Bartonella-infected stray cat population

Cited by 48 publications

References 35 publications

The effect of ecological and temporal factors on the composition of Bartonella infection in rodents and their fleas

The effect of ecological and temporal factors on the composition of Bartonella infection in rodents and their fleas

Bartonella henselae IgG antibodies are prevalent in dogs from southeastern USA

Cat-scratch disease in veterinary-associated populations and in its cat reservoir in Taiwan

Contact Info

Product

Resources

About