Lack of type-specific concordance between human papillomavirus (HPV) serology and HPV DNA detection in the uterine cervix and oral mucosa

Abstract

There is limited knowledge about longitudinal genotype-specific concordance between human papillomavirus (HPV) serology and co-existent presence of HPV DNA in the uterine cervix. The role of oral HPV infections in inducing serological response is unclear, as is the effect of HPV antibodies on the outcome of oral HPV infections. The present study is part of the Finnish Family HPV Study designed to evaluate dynamics of HPV infections within families. Here, we correlated the point prevalence of HPV6, 11, 16, 18 and 45 antibodies and concomitant genotype-specific HPV DNA detection in cervical and oral samples of 323 mothers during their 3 year (mean 37.5 months) follow-up. The mean age of these pregnant mothers at enrolment (third trimester) was 25.5 years. HPV antibodies were analysed with multiplex HPV serology and HPV genotyping was performed using a Multimetrix kit (Progen Biotechnik). There was no concordance between cervical DNA detection and co-existent seropositivity, and the same was true even in samples taken 12 months apart. Women who cleared their cervical HPV16 infection had the highest HPV16 antibody levels, whereas those who acquired incident HPV16 infections had the lowest antibody levels. Neither the presence nor the dynamics of oral HPV DNA had any correlation with HPV serology.

Seroprevalence of Human Papillomavirus (HPV) Type 6 and 16 Vary by Anatomic Site of HPV Infection in Men

“…Our finding is consistent with previous observations that HPV seroprevalence was not associated with concurrent detection of genital HPV DNA in healthy adults (5, 11, 12). Given the lower incidence of HPV 6 infection in the anus compared to the external genitalia (1.2 vs. 3.6 per 1000 person-months), and relatively comparable clearance at both sites (64.7% at 6-month visit vs. 50% at 6.4 months) previously reported for the HIM Study participants (8, 13), higher HPV 6 seroprevalence in men with anal HPV 6 infection compared to those with genital HPV 6 infection alone observed in the current study is unlikely caused by differences in acquisition and clearance of anal and genital HPV.…”

Anal, Penile, and Oral High-Risk HPV Infections and HPV Seropositivity in HIV-Positive and HIV-Negative Men Who Have Sex with Men

“…Possibly our study was underpowered to detect a significant independent association between oral HPV infection and seropositivity. A lack of concordance between oral HPV infection and type-specific serum antibodies among women has been described before [35]. Studies among men are scarce; one study suggested that tonsillar HPV infection may be associated with seropositivity in men, although this analysis was not performed on an HPV type-specific level [36].…”

HPV genotypes and their prognostic significance in head and neck squamous cell carcinomas

“…Cutoff value for each run and HPV-type was calculated on the basis of the signal of negative control: Cutoff = 1.5 × Background MFI + 5. All HPV16-positive samples were retested with our in-house bead-based HPV16-genotyping assay 26 to exclude HPV16 contamination.…”