If chronic osteomyelitis is not treated promptly and thoroughly, the wound will not heal for a long time, and the affected limb will be dysfunctional or disabled. In severe cases, it may even require amputation. In this article, a total of 50 patients with chronic osteomyelitis who meet the inclusion criteria were selected from January 2019 to March 2020 and were divided into two groups based on patient compliance, namely, a treatment group and a control group. The results of this group of studies showed that osteomyelitis is mostly manifested as limited diffusion, showing high signal on DWI, and ADC value is significantly higher than that of normal bone area. The normal bone area did not show obvious focal abnormal signals on the conventional MRI image. The ADC value of the measured patient’s osteomyelitis area was compared with the ADC value of the normal bone area. The ADC value was significantly higher than that of the normal bone area, and the difference was statistically significant. Studies have shown that membrane induction technology is superior to the bone handling group in terms of limb function scores and results satisfaction in the treatment of chronic osteomyelitis bone defects. Membrane induction technology has a low complication rate, a small number of X-ray examinations, a short healing time, and a high functional score. However, bone handling technology has a long treatment process, long fixation time and healing time, nail channel infection, joint stiffness, nerve damage, and many other complications.