Lactate dehydrogenase activity and isoenzyme patterns in skeletal muscle, fat, exocrine pancreas and isolated pancreatic islets of normal and obese-hyperglycaemic mice

Abstract

Summary. The total lactate dehydrogenase (LDtt) activity and LDH isoenzyme patterns of isolated pancreatic islets, acinar pancreas, fat tissue and skeletal muscle from mice with obese-hyperglycaemic syndrome were compared with those of corresponding tissues from lean littermates. --The pancreatic tissues of obesehyperglyeaemic animals exhibited higher LDH activity than corresponding tissues of normal animals. This increase in LDH activity was not parallel in islets and acinar tissue. --No significant difference in the LDIt activity of either skeletal muscle or fat tissue between the two groups of animals was found. --In animals with obese-hyperglycaemic syndrome, LDI-I isoenzyme patterns of pancreatic islets, aeinar pancreas and fat showed LDI-I 5 increase and LDH 4 decrease. LDH isoenzyme patterns of skeletal muscle showed the decrease of LDH 5 percentage. --The LDH 1, LDtt 2 and LDtI 3 percentages of the fat tissue were lower in animals with obese-hyperglyeaemie syndrome, but those of the skeletal muscle were lower in normal animals. --Results presented are consistent with the opinion that in obese-hyperglycaemie mice both endocrine and exocrine pancreatic tissues as well as fat tend to have an increase in the proportion of glycolysis in their intermediary metabolism, whereas the reverse tendency --towards an increase in the proportion of oxidative pathways --is valid for skeletal muscle. Activitg de la lactate dgshydroggnase et r@artition des iaoenzymes dana le muscle atrid, le tissu adipeux, le pancreas exocrine et lea ~lota paner~atiques isolda de souris normales et ob~se-hyperglycdmiquesRdsumd. L'activit6 de la lactate d@shydrog@nase totale (LDH) et la r@artition des isoenzymes de la LDtt dans les riots pancr6atiques isol~s, le pancrdas aeineux, le tissu adipeux et le muscle strid de souris ayant un syndrome ob~se-hyperglyc@mique, ont dr@ compar6es avee celles des tissus correspondants de souris non-ob~ses de re@rues parents. --Les tissus pancr6atiques des animaux ob@se-hyperglycdmiques montraient une activitd LDtt plus dlevde que les tissus correspondants des animaux normaux. Cette augmentation de l'activit6 de la LDH n'dtait pas parall~le dans les riots et le tissu acineux. --On n'a trouv6 aueune diff@renee significative dans l'activitd de la LDtt du muscle strid ou du tissu adipeux entre les deux groupes d'animaux. --Chez les animaux ayant un syndrome ob~se-hyperglyc@mique, la r@artition des isoenzymes de la LDI-I dans les riots pancrdatiques, le pancrdas acineux et le tissu adipeux, montrait une augmentation de la LDH 5 et une diminution de la LDH 4. La r@artition des isoenzymes de la LDtt du muscle strid montrait une diminution du pourcentage de la LDH 5. --Les pourcenrages de LDH 1, de LDH 2 et de LDH 3 du tissu adipeux dtaient plus bas chez les animaux ayant un syndrome obgse-hyperglyc@mique, mais eeux du musele stri6 @talent plus bas chez les animaux normaux. --Les r@sultats pr6sent6s sont en concordance avec l'opinion que chez les souris obgse-hyperglycgmiques, ~ la fois les tissus pancr6atiques e...