Lactate dehydrogenase and glutamic oxalic transaminase isozymes in heart muscle of forensic pathological cases.

Abstract

-275 -LDH and GOT isozyme patterns in the heart muscle of forensic pathological cases were investigated to find out the relationship between their changes and the causes of death. In most cases of death by violence, natural causes, poisoning and cold, the sum of LDH-1 and LDH-2 averaged about 80-90% of the total LDH, and GOTs was equal to or slightly lower than GOTm. In about two thirds of cases of asphyxia and drowning, LDH-1 and LDH-2 decreased and LDH-3, LDH-4 and LDH-5 increased. The activity of GOTs decreased and the ratio of GOTs/GOTm diminished. In neonate, LDH-3 showed the highest activity, followed by LDH-2 and LDH-1. GOTm was much less than GOTs. In most cases of cardiac death from unknown cause and cot death, LDH-3 increased remarkably, though it was less than LDH-1 and LDH-2, and GOTm became much less than GOTs. These findings revealed that there were some similarities in the LDH and GOT isozyme patterns in the heart muscle of the cardiac death from unknown cause and cot death and that of neonate.

Experimental model of sudden cardiac death from unexplained cause.

“…These changes of isozyme fractions have been investigated in the human heart muscle of sudden death cases (Suzuki et al 1978(Suzuki et al , 1980. In the experimental animals, the changes of these isozyme patterns except that of MDH seemed to be almost identical with the results obtained in the human heart muscle of sudden cardiac death cases from unexplained cause.…”

“…Electropholetic analysis of each isozyme was made after our previous papers (Suzuki et al 1978(Suzuki et al , 1980.…”

Creatine Phosphokinase and Malate Dehydrogenase Isozymes in Heart Muscle of Sudden Death Cases

“…However, these changes of CPK and MDH isozyme patterns, together with the changes of LDH and GOT isozyme patterns (Suzuki et al 1978), suggest that there are some similar changes in many other kinds of heart enzymes in cases of cardiac death from unknown cause and cot death.…”

“…Among these enzymes, those having characteristic isozyme patterns such as lactic dehydrogenase (LDH) and glutamic oxalic transaminase (GOT) were investigated in human hearts from medicolegal cases (Suzuki et al 1978), and some characteristic changes of their isozyme patterns were found in cases of cardiac death from unknown cause and cot death.…”