Lactate Dehydrogenase and Oxidative Stress Activity in Primary Open-Angle Glaucoma Aqueous Humour

Abstract

Lactate dehydrogenase (LDH) and lactate are some of the hypoxy biochemical parameters. Extracellular activity of this enzyme increases under the condition of oxidative stress, since the cell integrity can be disrupted during the lipid peroxidation process. Subsequently that leads to the increase level of the lactic acid and lactic acid salts. The objective of this investigation is establishing the level of LDH, LDH1 (HBDH) and the lactate concentration in aqueous humour in patients with primary open-angle glaucoma. Biochemical analysis have been made by enzymatic-colometric method (lactate) and UV-kinetic method (LDH and HBDH) in aqueous humour of 30 patients (42 eyes) with primary open-angle glaucoma (POAG) and 30 patients (40 eyes) with cataract (the control group). The increased values of lactate and the activity of LDH and HBDH enzyme in aqueous humour of POAG patients in correlation with the control group are the results not only of oxidative stress but also of hypoxy and the mitochondry oxidative function (p<0,001). The increased activity of the examined biochemical parameters in the aqueous humour of the POAG patients points to the fact that other mechanisms, besides IOP, have a role in glaucoma pathogenesis.

Effect of Aluminium and Aqueous extract of Rosmarinus officinalis on rat Brain: Impact on Neurobehavioral and Histological study

“…This event can be quantified in tissue culture settings by measuring the release of the intracellular enzyme lactate dehydrogenase (LDH). To confirm results of our histological study and detect cell damage and or death, we analyzed the level of this enzyme in the brain; Our results showed that after intoxication at short and long period by Al; the LDH levels increase significantly compared to control, indicating damaged membranes and cell necrosis [61][62]. Consistent with other results in this study, the Al+AER treated group induced decrease in LDH activity.…”

Protective Effect of Citrulline on the Hearts of Rats with Sepsis Induced by Cecal Ligation and Puncture

“…The isoenzymes of LDH include LDH1, LDH2, LDH3, LDH4, and LDH5, and the level of LDH1 in the myocardium is higher than the level of the other isoenzymes [ 25 ]. Finally, HBDH uses α -HBDH as a substrate, which reflects the sum of LDH1 and LDH2 activities [ 26 ].…”

Imidacloprid impedes mitochondrial function and induces oxidative stress in cotton bollworm, Helicoverpa armigera larvae (Hubner: Noctuidae)

“…In the present study, imidacloprid-induced oxidative stress in H. armigera larvae in a dose-and time-dependent manner as evidenced by increased levels of H 2 O 2 content, and lipid peroxidation in insecticide-fed larvae. Increased extracellular activity of LDH enzyme under oxidative stress condition was consequently observed due to the loss of cell integrity during the lipid peroxidation process (Jovanović et al 2010). In general, antioxidant enzymes are the potent scavengers of ROS, but the inhibition observed in the activities of CAT and SOD enzymes may be because of direct interaction of the insecticide with the enzymes, thereby resulting in increased levels of H 2 O 2 content and lipid peroxidation.…”