Lactate Dehydrogenase Isoenzymes

Wilkinson, J. H.

doi:10.1007/978-1-4613-3392-0_6

Cited by 6 publications

(4 citation statements)

References 240 publications

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“…The current practice for laboratory diagnosis of hemolysis or red blood cell destruction, based on a combination of elevated serum LD activity and bilirubin coupled with elevated reticulocyte count, is far from satisfactory. Serum LD concentration may also be elevated in hepatic, cardiac, pulmonary and placental diseases thus reducing its specificity for detecting hemolysis (3)(4)(5). Similarly, bilirubin concentration in serum may be elevated in intra-and extra-hepatic biliary disease and in severe hepatocellular dysfunction (6).…”

Section: Discussionmentioning

confidence: 98%

Erythrocyte adenylate kinase isoenzyme as a marker for hemolysis

Thomas

Murthy

1997

J. Clin. Lab. Anal.

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The presence in serum of adenylate kinase isoenzyme originating from erythrocyte can be useful as a marker for detecting hemolysis. We have presented preliminary evidence for identifying hemolytic anemia patients earlier by determining erythrocyte AK isoenzyme activity in serum (or plasma) rather than using measurement of plasma hemoglobin concentration. This test being quite specific for hemolysis should find use as a quick method for estimating the extent of in vivo hemolysis in hemolytic patients earlier than heretofore possible. J. Clin. Lab. Anal. 11:351–356, 1997. © 1997 Wiley‐Liss, Inc.

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Section: Discussionmentioning

confidence: 98%

Erythrocyte adenylate kinase isoenzyme as a marker for hemolysis

Thomas

Murthy

1997

J. Clin. Lab. Anal.

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“…They were then reacted for exactly 15 min in a substratecoupling agent mixture consisting of 40 mg of a-naphthyl butyrate and 100 mg of Fast Blue RR salt per 100 ml of 0.04 M pH 6.6 Tris-HC1 buffer at 37" C. The reaction was stopped with a 10% ethanol in 10% glacial acetic acid solution (Allen et a/., 1965). Gels were stained for LDH activity in a buffer corresponding to the tank buffer, to which lithium lactate, NAD, Nitro blue tetrazolium, and phenazine methosulfate had been added (Wilkinson, 1965). The LDH isoenzyme patterns were developed at 37" C for 30 min.…”

Section: Electrophoresismentioning

confidence: 99%

Isoenzyme and protein profiles of normal, leukemic and fetal mouse tissues

Tyndall

Allen

Friedman

et al. 1971

Intl Journal of Cancer

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Electrophoretic characterization of extracts from normal, leukemic and fetal mouse tissues indicated that infection with R L V results in the subsequent emergence of cells having profiles similar to fetal thymus and spleen tissues. Microdensitometric analyses showed diminution of prealbumin and haptoglobin esterase activity with concomitant predominance of postalbumin esterase activity in both fetal and leukemic thymus and spleen tissues. Both JLS V9 and J L S V6 infected cell cultures showed marked enhancement of postalbumin esterase activity. Leukemic and fetal thymus and spleen tissues also showed increased activity in LDH isoenzyme V, relative to the total L DH activity, compared to normal adult tissues. Increased relative activity in Band V LDH was also noted in infected V6 cells compared to uninfected cells. Increased amounts of proteins with electrophoretic mobilities similar to serum albumin and transferrin were evident in extracts of fetal and leukemic thymus and spleen tissues. Protein profiles of purified R L V showed a predominant band migrating similarly to serum albumin with minor bands migrating similarly to serum transferrin and haptoglobin.

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“…The measurements of LDH isoenzymes, particularly LD , , are used in man to detect cardiac necrosis, but its use in rats has not been widely reported, although tissue isoenzyme levels have been evaluated. 2 The correlation between HBDH and LDI + LD2 activity in the rat has not been evaluated and only one report was found on the distribution of HBDH in rat tissue. ' The distribution of HBDH, LDH and LDH isoenzymes in tissues is an important criterion of their clinical usefulness.…”

Section: Introductionmentioning

confidence: 99%

The evaluation of HBDH and LDH isoenzymes in cardiac cell necrosis of the rat

Barrett¹,

Harleman²,

Joseph³

1988

J of Applied Toxicology

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The total LDH, HBDH and LDH isoenzyme activities were assessed in a number of rat tissues. HBDH did not correlate with LD1 and LD2 isoenzyme activity in tissues with high HBDH activity. HBDH therefore cannot be used as a marker for cardiac necrosis in the rat. In rats treated with isoprenaline and SK&F 94120 at doses producing myocardial necrosis, only the LD1 isoenzymes showed any significant change but only within the first 24 h of treatment. No statistically significant differences were seen in the plasma AST, CK, CK-MB and total LDH activities.

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Lactate Dehydrogenase Isoenzymes

Cited by 6 publications

References 240 publications

Erythrocyte adenylate kinase isoenzyme as a marker for hemolysis

Erythrocyte adenylate kinase isoenzyme as a marker for hemolysis

Isoenzyme and protein profiles of normal, leukemic and fetal mouse tissues

The evaluation of HBDH and LDH isoenzymes in cardiac cell necrosis of the rat

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