Lactate dehydrogenase isoenzymes in dental pulp of rats according to stage of root development

Abstract

The objective of this study was to present a classification of the root development stage of female rat molar teeth and to evaluate the variation in the lactate dehydrogenase (LDH) activity and electrophoretic isoenzyme profile according to the stage of root development of the molar teeth. We also studied the LDH activity and isoenzymes of the pulp of incisor teeth. The stage of development of the rat first molar at the age of 15 days and that of the second molar at the age of 18 days was classified as the beginning of root formation. At the age of 15 days, the electrophoretic profile of the isoenzymes for the first molar showed a prevalence of LDH-1 followed by LDH-2. However, for the maxillary second molar there was a prevalence of LDH-4 followed by LDH-1, while for the mandibular second molar LDH-1 predominated followed by LDH-2 and LDH-4. From 18 days of age, the prevalence was always of LDH-1. The electrophoretic profile of LDH isoenzymes from the pulp of the incisor teeth at the ages studied (25 and 60 days) showed the following order of prevalence: LDH-1 > LDH-2 > LDH-3 > LDH-4 > LDH-5. These results suggest that there are variations in the prevalence of the various forms of LDH isoenzymes in the dental pulp of rats according to the developmental stage of the root.

Impact of hypofunctional occlusion on upper and lower molars after cessation of root development in adult mice

“…In contrast to the lower molars, the mesial root of the upper molars were shown to over erupt without any change in length. This is in contrast to previous experiments on young rats that showed an increase in root length in the upper jaw (21).…”

“…With the exception of the last study, hypofunctional occlusion has mainly been studied in young rats prior to the completion of root development and the cessation of the HERS, which occurs around 7 weeks after birth (postnatal day P50)) (21). Therefore many of the experiments were conducted while normal root development was still occurring.…”

Histological and histometric aspects of radicular dentinogenesis in upper first molars of fluoxetine treated rats

“…In the present study, for both 20 and 45-days-old animals, it was observed that the development of dentin, pre-dentin and root odontoblasts layer in thickness and extension are compatible with dentinogenesis phase. These data corroborate those described by Losso in 2003, in which the chronology of the rhizogenesis of the first upper molars of rats indicated that on the twenty-first day of life the root is approximately two to three thirds; at forty days old the root is almost completely formed and from 60 days the root is already fully formed.…”

Quantification of Lactate-Dehydrogenase and Cell Viability in Postmortem Human Dental Pulp

“…LDH was quantified in this study because it is an intracellular enzyme actively participating in the metabolism of tissues having a high demand for oxygen, such as dental pulp (9,19,24). LDHs importance for a cell lies in its capacity to turn lactate into piruvate, which could be transformed into glucose.…”