Lactate dehydrogenase (LDH) Isozymes in Some Species of Fresh Water, Euryhaline and Salt Water Teleosts from the Po Plain and its Sea-Coasts

Callegarini, Carlo; Ricci, Daniele

doi:10.1080/11250007309427478

Cited by 9 publications

(3 citation statements)

References 17 publications

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“…The interpretation of electrophoretic patterns was achieved by application of the Mendelian law of monohybrid segregation and the use of statistical rules to combine the enzyme subunits. All data were compared with the previous descriptions reported in the literature concerning Z. ophiocephalus (Callegarini & Ricci, 1973;McKay & Miller, 1991;Miller et al, 1994;Sorice & Caputo, 1999), other Gobiidae (Wallis & Beardmore, 1984a, b;Pezold & Grady, 1989;Aizawa et al, 1994) and other fish (Basaglia, 1989;Farias et al, 1997). In this way, 18 loci were postulated for the 12 enzyme systems assayed (Table 2).…”

Section: Gene-enzyme Systemsmentioning

confidence: 97%

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Low variation at allozyme loci and differences between age classes at microsatellites in grass goby (Zosterisessor ophiocephalus) populations

et al. 2007

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Allozymes and microsatellites were used to assess the level and distribution of genetic variation in grass goby population samples collected from the Venice Lagoon between October 2001 and May 2002. Eighteen enzymatic loci were examined in 434 individuals, 14 of which resulted to be monomorphic, and 4 (GPI-B*, LDH-B*, PGM-A*, PGM-B*) showed 2 alleles scored in 6 individuals only. Comparison with previous data suggests that genetic variation has been eliminated in the Venice Lagoon population during the last few years at three loci. In contrast, analysis of 11 microsatellites in a subset of 192 individuals revealed substantial molecular variation. Analysis of molecular variance showed a lack of genetic differentiation inside the lagoon with respect to site and date of collection, sex, and level of pollution. Significant variation in allelic frequencies was found at microsatellite loci when small (one year old) males were compared to large males (two and three years old), suggesting that a complex population dynamics occurs in this species. The very low level of polymorphism of allozymes could be due to the evolutionary history of the species, or, considering the difference between small and large males, could be the result of recent effects of drift. The second hypothesis is supported by the comparison with previous allozyme studies of the species in the same area, that suggests that loss of heterozygosity at three loci occurred in the last 10 years

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Section: Gene-enzyme Systemsmentioning

confidence: 97%

“…Callegarini & Ricci (1973) have analysed variability of the LDH locus, while more recent studies focused on phylogeny of Mediterranean gobies using both biochemical (McKay & Miller, 1991;Miller et al, 1994;Sorice & Caputo, 1999) and mitochondrial markers (Penzo et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Low variation at allozyme loci and differences between age classes at microsatellites in grass goby (Zosterisessor ophiocephalus) populations

et al. 2007

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“…After the ope- cations given by TORTONESE (1972,1975) and SOLJAN (1963). Electrophoresis of hemoglobins and glucose-6phospho-dehydrogenase (G-6-PD) was done on starch gel at pH8.6 using the discontinuous system of buffers indicated by POULIS (1957) slightly modified as previously described (CAL-LEGARINI, 1966); electrophoresis of isoenzimes mas also done on starch gel but using the continuous system of buffers indicated by SYNER and GOODMAN (1966); here too the technique employed has previously been described at length (CALLEGARINI and RICCI, 1973).…”

Section: Hiaterial and Hlethodmentioning

confidence: 99%

Biochemical characteristics of mugilids in the lagoons of the Po delta

Callegarini

Basaglia

1978

Bolletino di zoologia

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Electrophoretic method has been applied to biochemical classification of hfugilids in the Lagoons of the Po delta. Among the various proteins analysed the one which turned out to be the most useful was the lactate dehydrogenase enzyme (LDH) because it presents different patterns in the five species living in the above mentioned lagoon and does not show any variations in the course of ontogenesis. This caracteristic makes it possible to identify stocks of fry to be put into closed waters for subsequent cultivation. hloreover this method allows one to make certain phylogenetic speculations with regard to the origin of these species.

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Differential survivorship of allozyme genotypes in mosquitofish populations exposed to copper or cadmium

Chagnon¹,

Guttman²

1989

Enviro Toxic and Chemistry

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Laboratory stocks of mosquitofish (Gambusia affinis) were acutely exposed to copper (0.16–0.24 mg/L) or cadmium (6.7–9.5 mg/L) for 48 h using a static renewal system. Allozyme genotypes at three polymorphic loci: phosphoglucomutase‒2 (PGM‒2), glucose phosphate isomerase‒2 (GPI‒2), and isocitrate dehydrogenase‒2 (ICD‒2) were resolved using a modified starch gel (13%) electrophoretic technique. Differential survivorship of allozyme genotypes was analyzed using a Wilcoxon matched pair signed‒rank test. Copper significantly (p < 0.05) decreased the overall survivorship of fish heterozygous at the GPI‒2 locus. The frequency of the (ab) genotype was significantly higher in the dead fraction of the test populations than in the surviving fraction. The frequencies of the (aa) genotype and the (a) allele at the ICD‒2 locus were significantly higher in female fish surviving exposure to copper than in nonsurviving females. The frequencies of the (bb) genotype and the (b) allele at the GPI‒2 locus were significantly higher in fish surviving exposure to cadmium than in nonsurvivors. These results indicate that some electrophoretically detectable allozymes are more sensitive than others to the toxic effects of environmental pollutants. This suggests that the genetic structure of natural populations should be considered for use as a biological monitor of environmental pollution.

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Lactate dehydrogenase (LDH) Isozymes in Some Species of Fresh Water, Euryhaline and Salt Water Teleosts from the Po Plain and its Sea-Coasts

Cited by 9 publications

References 17 publications

Low variation at allozyme loci and differences between age classes at microsatellites in grass goby (Zosterisessor ophiocephalus) populations

Low variation at allozyme loci and differences between age classes at microsatellites in grass goby (Zosterisessor ophiocephalus) populations

Biochemical characteristics of mugilids in the lagoons of the Po delta

Differential survivorship of allozyme genotypes in mosquitofish populations exposed to copper or cadmium

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