Lactic acid bacteria from healthy oral cavity of Thai volunteers: inhibition of oral pathogens

Abstract

The aims of the present study were to screen and characterize the antimicrobial lactic acid bacteria which were isolated from healthy oral cavities of Thai volunteers, and to characterize their inhibiting substances. Among 3790 isolates (suspected to be lactic acid bacteria) from 130 volunteers, ®ve showed an appreciable effect against Sarcina lutea ATCC 9341, Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Streptococcus mutans DTMU 1, Strep. salivarius DTMU 1, Strep. sanguis DTMU 1, Candida albicans ATCC 13803 and C. albicans DTMU 2, as well as the oral pathogens. These antimicrobial isolates included L17 and N14 which showed the antibacterial activity, D14 which showed the anticandidal activity, and D6 and N8 which showed both the antibacterial and anticandidal activities. The isolates were later found to be facultative anaerobic, Gram-positive, non-spore-forming, non-capsuleforming and catalase-negative bacilli. They could utilize casein but could not hydrolyse starch, and they produced hydrogen peroxide and bacteriocins. Their antimicrobial potentials were found to be affected by pH, catalase, proteolytic enzymes and temperature. The activity was partially inactivated after catalase treatment, signi®cantly declined at pH ³9á0 or after trypsin and pepsin treatments, and also reduced after heating at ³100°C. However, the antimicrobial activity of these ®ve isolates was somewhat resistant to heat. When the isolates were tested for their antimicrobial sensitivity, they were shown to be sensitive to a number of antimicrobial agents. The ®nal identi®cation revealed that D6, D14 and N14 were Lactobacillus paracasei subsp. paracasei, and L17 and N8 were Lact. rhamnosus.

The in vitro Activity of Vaginal Lactobacillus With Probiotic Properties Against Candida

“…Sookkhee et al [25] recently found that the anticandidal activity of some oral Lactobacillus strains was not inhibited by heat treatment, which also suggested alternative mechanisms of inhibition. In fact, several antifungal substances such as cyclic dipeptides, pyroglutamic acid and other substances of low molecular mass, such as lactones, produced by different Lactobacillus species, have recently been characterized [19,26].…”

Effect of Porphyromonas gingivalis and Lactobacillus acidophilus on Secretion of IL1B, IL6, and IL8 by Gingival Epithelial Cells

“…Moreover, during the past few years, several investigators have reported the use of probiotics for the maintenance of oral health [12,16]. Some studies have confirmed that lactobacilli can inhibit the growth of periodontopathogens, including P. gingivalis, Prevotella intermedia, and Aggregatibacter actinomycetemcomitans [17,18]. In our previous study, we have also reported the inhibitory effect of Lactobacillus acidophilus on the growth of P. gingivalis [19].…”

A tolerant lactic acid bacteria, Lactobacillus paracasei, and its immunoregulatory function

“…Lactobacillus paracasei constitutes a substantial part of the Lactobacillus flora of the human intestinal mucosa (Ahrne et al 1998). Studies have shown that certain strains of L. paracasei can produce anti-bacteria and anti-yeast compounds in the human vagina and oral cavity (Ocaña et al 1999;Sookkhee et al 2001;Atanassova et al 2003). In addition, the strain has played a general beneficial health role as a probiotic, and the important traits of this species include antihypertensive, antiinflammatory, diuretic, sedative, and anti-diabetic (Adeghate and Ponery 2002;Jahreis et al 2002;Wang et al 2004;Komatsuzaki et al 2005).…”