Lactic Acid Bacteria | Streptococcus thermophilus

Harnett, James; Davey, Graham P.; Patrick, Asim; Caddick, C.; Pearce, L. E.

doi:10.1016/b978-0-12-374407-4.00268-5

Cited by 11 publications

(10 citation statements)

References 7 publications

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“…), it is interesting to think about the usefulness of phages DT1 and DT6 as biocontrol tools in milk fermented by these mesophilic bacteria, either alone or as part of mixed cultures (Harnett et al . ). However, some parameters like temperature evolution and pH decrease during the fermentation process must be considered.…”

Section: Resultsmentioning

confidence: 97%

“…Phages DT1 and DT6 were effective to reduce E. coli counts without compromising the performance of Streptococcus thermophilus, used as starter culture. In this sense, as Lactococcus lactis is even more extensively used than Streptococcus thermophilus worldwide (Mills et al 2011), it is interesting to think about the usefulness of phages DT1 and DT6 as biocontrol tools in milk fermented by these mesophilic bacteria, either alone or as part of mixed cultures (Harnett et al 2011). However, some parameters like temperature evolution and pH decrease during the fermentation process must be considered.…”

Section: Biocontrol Tests During Milk Fermentationmentioning

confidence: 99%

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Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation

Tomat

Mercanti

Balagué

et al. 2013

Letters in Applied Microbiology

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Significance and Impact of the Study: Coliphages DT1 and DT6, isolated from faeces and selected on the basis of their host range, showed to be valuable tools for the control of pathogenic Escherichia coli during milk fermentation, without compromising the starter culture performance. Both phages, either individually or as a cocktail, may function as an extra safety barrier beyond traditional pasteurization, effectively reducing O157:H7 Shiga toxin-producing Escherichia coli (STEC) counts during early growth, thus avoiding Shiga toxin production and accumulation. AbstractTwo bacteriophages, isolated from faeces, were assayed as biocontrol agents of pathogenic Escherichia coli during milk fermentation. Phage DT1 was tested on the strain E. coli DH5a, one enteropathogenic E. coli (EPEC) strain and one Shiga toxigenic E. coli O157:H7 (STEC) strain. Phage DT6 was tested on two STEC strains (O157:H7 and non-O157). One additional assay was performed by using a cocktail of both phages against the O157:H7 STEC strain. Streptococcus thermophilus 10-C, the strain used as lactic starter, reached 10 9 CFU ml À1 after 4 h, while pH values fell to 4Á5 after 8 h, regardless of the presence of E. coli strains and/or phages. In absence of phages, E. coli strains reached 4-6 log CFU ml À1 at 5-6 h. Escherichia coli DH5a and O157:H7 STEC strains were rapidly and completely inactivated by phage DT1 and phage cocktail, respectively, while O157:H7 STEC was completely inactivated either by DT1 or by DT6, after 8 h. The EPEC strain was not detected at 1 h (<10 CFU ml À1 ) but grew afterwards, though at lower rates than without phage. For non-O157:H7 STEC, reductions lower than 1 log CFU ml À1 were observed for all sampling times. Phages DT1 and DT6, either individually or as a cocktail, effectively reduce O157:H7 STEC counts during milk fermentation, without compromising the starter culture performance.

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Section: Resultsmentioning

confidence: 97%

Section: Biocontrol Tests During Milk Fermentationmentioning

confidence: 99%

Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation

Tomat

Mercanti

Balagué

et al. 2013

Letters in Applied Microbiology

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“…As mentioned above, we aimed to test the impact of temperature on Cas9/Cas12a activity, as these nucleases were isolated from different bacteria having an optimal growth range, from 35 °C to 45 °C (Angelos, 2010;Chang et al, 2010;Harnett et al, 2011;Hudson, 2014;Gera and McIver, 2013), and these temperatures are substantially higher than the 20 °C-28 °C temperature range that is best suited for plant tissue culture and transformation. In fact, when solely assessed visually, nuclease activity appears to be reduced at room temperature (22 °C-28 °C) (Moreno-Mateos et al, 2017;LeBlanc et al, 2018;Malzahn et al, 2019;Milner et al, 2020;Schindele and Puchta, 2020), with Cas12a being the most negatively impacted.…”

Section: Discussionmentioning

confidence: 99%

Editing efficiencies with Cas9 orthologs, Cas12a endonucleases, and temperature in rice

2023

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The advent of CRISPR-Cas technology has made it the genome editing tool of choice in all kingdoms of life, including plants, which can have large, highly duplicated genomes. As a result, finding adequate target sequences that meet the specificities of a given Cas nuclease on any gene of interest remains challenging in many cases. To assess target site flexibility, we tested five different Cas9/Cas12a endonucleases (SpCas9, SaCas9, St1Cas9, Mb3Cas12a, and AsCas12a) in embryogenic rice calli from Taipei 309 at 37°C (optimal temperature for most Cas9/Cas12a proteins) and 27°C (optimal temperature for tissue culture) and measured their editing rates under regular tissue culture conditions using Illumina sequencing. StCas9 and AsCas12 were not functional as tested, regardless of the temperature used. SpCas9 was the most efficient endonuclease at either temperature, regardless of whether monoallelic or biallelic edits were considered. Mb3Cas12a at 37°C was the next most efficient endonuclease. Monoallelic edits prevailed for both SaCas9 and Mb3Cas12a at 27°C, but biallelic edits prevailed at 37°C. Overall, the use of other Cas9 orthologs, the use of Cas12a endonucleases, and the optimal temperature can expand the range of targetable sequences.

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“…Streptococcus thermophilus S. thermophilus is a gram-positive bacteria that ferments sugars like lactose, fructose, sucrose and glucose. 20 They are used in the manufacturing of yogurt, hard Italian and Swiss cheeses by its fermentation activity. 21 They are highly resistive against bacteriophages and especially the CRISPR system, 22 and relatively sensitive to antibiotics and sanitisers.…”

Section: Dairy Industrymentioning

confidence: 99%

A review article on the microbes used for industrial and waste water treatment purposes

Haneef,

Ameen K,

Uvais P K

2023

IJMR

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The term microbe is an umbrella term used to denote organisms of micro size that mainly includes bacteria, fungus and protists. This paper is focused on collecting the informations and giving an overview on the microbes and its applications in treatment of contaminated water, and it ’s industrial application for the production and enhancement in industrial products. The methodology- searching the databases like “PubMed”, “Research gate” and “google scholar” using the keywords like “microbes of dairy industry”, “bacteria for leather industry” and “microbial application in industries”. The microbes were selected for listing by coinciding different articles retrieved from the databases by making the names of the microbes as the keywords. findings are- the microbes like and some others contribute to dairy industry, , and , and some lipase and protease yielding bacteria are used in food, medical leather industries respectively. Moreover, the application of and in treatment of contaminated water are also included in this paper. This review article is highly recommendable and helpful for various industries to find out the microbes they need to culture and treat contaminated water.

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Lactic Acid Bacteria | Streptococcus thermophilus

Cited by 11 publications

References 7 publications

Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation

Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation

Editing efficiencies with Cas9 orthologs, Cas12a endonucleases, and temperature in rice

A review article on the microbes used for industrial and waste water treatment purposes

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