Lactic Acid Bacteria Used in Inoculants for Silage as Probiotics for Ruminants

Abstract

Many studies have shown the beneficial effects on ruminant performance of feeding them with silages inoculated with lactic acid bacteria (LAB). These benefits might derive from probiotic effects. The purpose of the current study was to determine whether LAB included in inoculants for silage can survive in rumen fluid (RF), as the first step in studying their probiotic effects. Experiments were conducted in the United States and Israel with clarified (CRF) and strained RF (SRF) that were inoculated at 10(6)-10(8) microorganisms/mL with and without glucose at 5 g/L. RF with no inoculants served as control. Ten commercial inoculants were used. The RF was incubated at 39 degrees C and sampled in duplicates at 6, 12, 24, 48, 72, and 96 h for pH and LAB counts. The results indicate that with glucose the pH of the RF decreased during the incubation period. In the SRF, the pH of the inoculated samples was higher than that of the controls in most cases. This might be a clue to the mechanism by which LAB elicit the enhancement in animal performance. LAB counts revealed that the inoculants survived in the RF during the incubation period. The addition of glucose resulted in higher LAB counts.

Pengaruh Penambahan Molases dan Isolat Bakteri Asam Laktat dari Cairan Rumen Sapi PO Terhadap Kualitas Silase Rumput Gajah (Pennisetum purpureum)

“…Inokulum bakteri asam laktat merupakan aditif yang populer diantara adtif lainnya seperti asam, enzim dan sumber karbohidrat (Bolsen et al, 1995). Bahkan inokulum silase ini dapat juga berpeluang sebagai probiotik karena sifatnya yang masih dapat bertahan hidup sampai bagian lambung utama dari ruminansia yaitu rumen (Weinberg et al, 2004) …”

The Use of Probiotic Strains as Silage Inoculants

“…This is due to a higher acetic acid content, which stops yeast growth. Increased concentrations of acetic acid in silage treated with BSP had a positive effect on the aerobic stability of the silage [24,55].…”

The effect of biological and chemical additives on the chemical composition and fermentation process of Dactylis glomerata silage

“…The exceptional activities of the biological inoculants include: rapid production of LA, improvement of the aerobic stability of silage due to production of acetic acid (Kleinschmit & Kung, 2006b), detoxification of mycotoxins, as well as inhibition of pathogenic bacteria or fungi undesirable in silage (Li & Nishino, 2011), which also prevent probiotic action (Weinberg et al, 2004).…”