Lactic Dehydrogenase and Succinic Dehydrogenase in Psoriasis

Abstract

RECENT biochemical and histochemical investigations in })soriasi8 have indicated, directly or indirectly, enhanced respiration and glycolysis (Gans. 1962; Braun-Falco, 1958; Rippa and Vignalli, 1958; Leonhardi, 1961 and Hasimoto e( fl?., 19ti4).Of the quantitative studies, those establishing a rise of the respiratory quotient of the epidermis in the psoriatio lesion as compared with the patients normal skin (Braun-Falco, 1958; Leonhardi, 1961; Blazkova, 1963) are most important. Corres}>ondingly, there is a noticeable decrease of the glueose level (JVlonaeelii and RibufFo, Neumann and Blazkova, 1963; Rothmau. 19^4) as well as that of lactic acid (Neumann and Blazkova) in the psoriatic lesion in comparison with the healthy skin of the psoriatic patient. Among the other oxidative and glycolytie enzymes, elevated values of glucose-6-phoHphatr dcliydrogenaso and of 6-phosphogluconate dehydrogenase were recently found, whereas no significant variations of phosphohexose, isomerase and aldolase r-ould be established (Rippa and Vignalli, 1965).By histochemical methods, raised values were found for succinic dehydi'ogenase (Braun-Falco, 1958; Hashimoto et al, 1964), eytochromoxydase (Brauii-Faleo, 1958), malic dehydrogenase, laetie dehydrogenase (Hashimoto el al.. 1964), thus indicating a higher level of sugar catabolism.In our present study, the following enzymes were determined quantitatively : aldolase, laetie dehydrogenase and succinic dehydrogenase. IN VESTIO ATION.As in our jJiwiouH iiivcstigatioiis, excisions from It-siuns and troiii t'orri-spotuliiiĥ ealthy skin in psoriatics were used for the determination of enzymes. The tissue was frozen uitli kelene and a circular excision of 0-5 cm. diameter was made by nican(< of rotary punch. The corium was cut off with scissors and stored in the rofrigcrator. * Present address: Department of Dermatologj', Laaarettet, BorSs, Sweden.

Significance of Elevated Serum LDH (Lactate Dehydrogenase) Activity in Atopic Dermatitis

“…In contrast, the distribution patterns in fat tissue and the horny layer differ from those of epidermis and dermis; LDHS could not be detected in the horny layer (12). In a Discussion Serum and skin LDH levels have been measured in patients with various skin diseases (2)(3)(4)(5)(6)(7)(8); examples include cutaneous malignant and benign tumors, erythroderma, psoriasis vulgaris and viral infections. The association of high levels of serum LDH and liver metastases in advanced stage patients has been emphasized by several authors (10,11).…”

“…Isoenzyme analysis of LDH indicates the origin of elevated levels (1). Serum LDH levels have also been reported to be elevated in various skin diseases (2)(3)(4)(5)(6)(7)(8). Recent studies (3,4) suggested that serum LDH5 elevations in eczema-associated erythroderma were correlated with the epidermal cell damage.…”

Kohlenhydratstoffwechsel bei der Psoriasis vulgaris

“…Im folgenden ergaben sich sodann aus zahlreichen Einzelbestimmungen von Enzymen (2,15,34,35,79,87,94,113) und Substraten (21,31,60,78,113,119) in psoriatisch veränderter Epidermis bzw. in psoriatischen Schuppen Hinweise auf eine Aktivitätssteigerung des Kohlenhydratstoffwechsels.…”

A Study of the LDH Level and the LDH Isoenzyme Pattern in Cantharidin Blister Fluid in Normal Skin and in Psoriasis

“…The superficial layer of the cutis (papillary layer) ex hibits a marked vasodilatation with an inflammatory infiltrate. Sev eral investigators have dem onstrated that nearly all the enzymes th at are present in the epidermis of psoriasis exhibit an enhanced activity, especially of LDH (Halprin, 1966 andNeumann, 1967).…”