Lactobacilli inhibit cervical cancer cell migration in vitro and reduce tumor burden in vivo through upregulation of E-cadherin

Abstract

The present study was designed to investigate the antitumor effects of Lactobacillus and the potential mechanisms. Cell Counting Kit-8 (CCK-8) assays were carried out to determine suitable doses for investigating the inhibitory effect of lactobacilli on cell migration ability of HeLa and U14 cells in vitro. In addition, western blot assays were performed to investigate the possible mechanisms corresponding to its antitumor effects. Furthermore, a xenograft mouse model was established for investigating the E-cadherin expression in tumor tissues after treatment with lactobacilli. Our results showed that live lactobacilli [multiplicity of infection (MOI) of 1,000:1] significantly possessed inhibitory effects on cell migration ability of cervical cancer cells. Lactobacilli (MOI: 1,000:1) significantly upregulated E-cadherin expressions in HeLa and U14 cells (p<0.05). On the contrary, our results showed that inactivated lactobacilli could not affect the E-cadherin expression levels in HeLa and U14 cells. Similar to the western blot assay, immunohistochemistry results also indicated that lactobacilli treatment significantly upregulated E-cadherin in tumor tissues (p<0.05). In conclusion, our results above suggest that lactobacilli have the potential for inhibiting the migratory ability of cervical cancer cell lines, and the possible pharmacological mechanism may be closely related to the upregulation of E-cadherin.

Role of <em>Lactobacillus</em> in cervical cancer

“…Many clinical studies found that a decline in the quantity and activity of Lactobacillu s was involved in the initiation and progression of cervical cancer, which provides a novel insight into the use of probiotics to prevent cervical cancer. 47 , 54 , 58 Some experimental studies found that Lactobacillu s and its metabolites inhibit the proliferation of cervical cancer cells by regulating cancer-related genes or through an immunological mechanism. These studies provide a theoretical basis for further clinical application of Lactobacillus in cervical cancer.…”

Long Intervening Noncoding 00467 RNA Contributes to Tumorigenesis by Acting as a Competing Endogenous RNA against miR-107 in Cervical Cancer Cells

“…E-cadherin and N-cadherin are well known to be vital for normal cell adhesion, homeostasis, and tissue integrity. Recently, Li et al 31 reported that elevation of E-cadherin relieves tumor burden and suppresses migration of cervical cancer cells. MMPs are related with HPV infections in cervical cancer patients, among which MMP2 and MMP9 are associated with poor prognoses in patients with invasive cervical cancer, and their knockdown decreases migration of cervical cancer cells.…”

Lactobacillus casei Zhang Counteracts Blood-Milk Barrier Disruption and Moderates the Inflammatory Response in Escherichia coli-Induced Mastitis

“…Lactobacillus is an important probiotic that contributes to the immunity and health of animals. Their probiotic properties play an important role in regulating gut microbiota ( Igor et al, 2019 ), relieving intestinal diseases ( Sanders et al, 2019 ), and enhancing body immunity ( Garcia-Castilla et al, 2019 ) in addition to other functions ( da Costa et al, 2016 ; Li et al, 2017 ). Lactobacillus is known to protect hosts against intramammary infection in both humans and cows by oral or intramammary administration ( Jiménez et al, 2008 ; Arroyo et al, 2010 ; Fernández et al, 2016 ), but the mechanism of this protective effect remains unclear.…”