Lactobacillus Decelerates Cervical Epithelial Cell Cycle Progression

Abstract

We investigated cell cycle progression in epithelial cervical ME-180 cells during colonization of three different Lactobacillus species utilizing live cell microscopy, bromodeoxyuridine incorporation assays, and flow cytometry. The colonization of these ME-180 cells by L. rhamnosus and L. reuteri, originating from human gastric epithelia and saliva, respectively, was shown to reduce cell cycle progression and to cause host cells to accumulate in the G1 phase of the cell cycle. The G1 phase accumulation in L. rhamnosus-colonized cells was accompanied by the up-regulation and nuclear accumulation of p21. By contrast, the vaginal isolate L. crispatus did not affect cell cycle progression. Furthermore, both the supernatants from the lactic acid-producing L. rhamnosus colonies and lactic acid added to cell culture media were able to reduce the proliferation of ME-180 cells. In this study, we reveal the diversity of the Lactobacillus species to affect host cell cycle progression and demonstrate that L. rhamnosus and L. reuteri exert anti-proliferative effects on human cervical carcinoma cells.

Fermented Dairy Products Modulate Citrobacter rodentium–Induced Colonic Hyperplasia

“…Pretreatment of mice with FDP-A and -B significantly reduced colonic hyperplasia, which is routinely used as a marker of intestinal inflammation in the CR model. Importantly, L. rhamnosus GG has been demonstrated to reduce cell cycle progression in cancer cells in vitro and, following a single treatment, induces epithelial cell proliferation in Drosophila melanogaster , mice, and humans [ 39 – 41 ]. Collectively, these data suggest that treatment of mice with Lactobacillus species (including L. rhamnosus ) in the form of FDP-A can antagonize colonic epithelial cell hyperproliferation.…”

Uncovering microbial duality within human microbiomes: A novel algorithm for the analysis of host-pathogen interactions

“…In this microbiome, we identified cell cycle, metabolism and DNA repair as the pathways being impacted the most. Such results also have literature support, as some Lactobacilli species were shown to decelerate cell cycle progression in the cervix, while a single Lactobacillus species was found to transiently accelerate cellular division in the human host [17].…”

Down-regulation of TSGA10, AURKC, OIP5 and AKAP4 genes by Lactobacillus rhamnosus GG and Lactobacillus crispatus SJ-3C-US supernatants in HeLa cell line

“…rhamnosus has anti-proliferative effects on ME-180 cell line (a human cervical epithelial-like adenocarcinoma cell line) via moderating cell cycle progression. Treatment with this Lacto bacilli resulted in accumulation of host cells in G1 phase through enhancement of expression and nuclear accumulation of p21 [30]. Anti-proliferative effect of L. crispatus SJ-3C-US has been reported in MDA-MB-231 cell line, which has been accompanied by down-regulation of ODF4, PIWIL2, RHOXF2, and TSGA10 [15].…”