Lactobacillus reuteri BM53-1 Produces a Compound That Inhibits Sticky Glucan Synthesis by Streptococcus mutans

Noda, Masafumi; Sugihara, Naho; Sugimoto, Yoshimi; Hayashi, Ikue; Sugimoto, Sachiko; Danshiitsoodol, Narandalai; Sugiyama, Masanori

doi:10.3390/microorganisms9071390

Cited by 10 publications

(4 citation statements)

References 56 publications

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“…Moreover, L. reuteri BM53-1 was found to produce a short extracellular polysaccharide with a molecular weight of about 30 kDa that impeded the production of sticky β-glucans by S. mutans and consequently the biofilm formation. In particular, the authors suggested that it acts by lowering the expression of gtfD that is necessary to give sickness to insoluble glucans produced in large amounts during the initial attachment of S. mutans on the surface ( 57 ).…”

Section: Beyond Probiotics: Lactobacillus Derivativesmentioning

confidence: 99%

Lactobacilli as Anti-biofilm Strategy in Oral Infectious Diseases: A Mini-Review

Giordani

Parolin

Vitali

2021

Front. Med. Technol.

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The spread of biofilm-related diseases in developed countries has led to increased mortality rates and high health care costs. A biofilm is a community of microorganisms that is irreversibly attached to a surface, behaving very differently from planktonic cells and providing resistance to antimicrobials and immune response. Oral diseases are an excellent example of infection associated with the formation of highly pathogenic biofilms. It is generally accepted that, when the oral homeostasis is broken, the overgrowth of pathogens is facilitated. Among them, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans are the main etiological agents of periodontitis, while Streptococcus mutans is strongly associated with the onset of dental caries. Other microorganisms, such as the fungus Candida albicans, may also be present and contribute to the severity of infections. Since the common antibiotic therapies usually fail to completely eradicate biofilm-related oral diseases, alternative approaches are highly required. In this regard, the topical administration of probiotics has recently gained interest in treating oral diseases. Thus, the present mini-review focuses on the possibility of using Lactobacillus spp. as probiotics to counteract biofilm-mediated oral infections. Many evidence highlight that Lactobacillus living cells can impede the biofilm formation and eradicate mature biofilms of different oral pathogens, by acting through different mechanisms. Even more interestingly, lactobacilli derivatives, namely postbiotics (soluble secreted products) and paraprobiotics (cell structural components) are able to trigger anti-biofilm effects too, suggesting that they can represent a novel and safer alternative to the use of viable cells in the management of biofilm-related oral diseases.

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Section: Beyond Probiotics: Lactobacillus Derivativesmentioning

confidence: 99%

Lactobacilli as Anti-biofilm Strategy in Oral Infectious Diseases: A Mini-Review

Giordani

Parolin

Vitali

2021

Front. Med. Technol.

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“…Second, this study only explored the effect of AS vicK on a single species of S. mutans , while the dental plaque biofilm in the oral cavity is a habitat for a variety of bacteria, and the interaction of multiple microorganisms affects the balance of the microecology. For example, Gtfs can bind to oral microbes such as Lactobacillus and Candida albicans ( C. albicans ), affecting their EPS production [ 53 , 54 ] and enhancing the cariogenicity of symbiotic biofilms [ 55 ]. The vesicles of S. mutans containing Gtfs can also increase C. albicans biofilm formation by increasing EPS production [ 56 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

Exopolysaccharides metabolism and cariogenesis of Streptococcus mutans biofilm regulated by antisense vicK RNA

Sun

Chen

et al. 2023

Journal of Oral Microbiology

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Background Streptococcus mutans ( S. mutans ) is a pivotal cariogenic pathogen contributing to its multiple virulence factors, one of which is synthesizing exopolysaccharides (EPS). VicK, a sensor histidine kinase, plays a major role in regulating genes associated with EPS synthesis and adhesion. Here we first identified an antisense vicK RNA (AS vicK ) bound with vicK into double-stranded RNA (dsRNA). Objective This study aims to investigate the effect and mechanism of AS vicK in the EPS metabolism and cariogenesis of S. mutans . Methods The phenotypes of biofilm were detected by scanning electron microscopy (SEM), gas chromatography-mass spectrometery (GC-MS) , gel permeation chromatography (GPC) , transcriptome analysis and Western blot. Co-immunoprecipitation (Co-ip) assay and enzyme activity experiment were adopted to investigate the mechanism of AS vicK regulation. Caries animal models were developed to study the relationship between AS vicK and cariogenicity of S. mutans. Results Overexpression of AS vicK can inhibit the growth of biofilm, reduce the production of EPS and alter genes and protein related to EPS metabolism. AS vicK can adsorb RNase III to regulate vicK and affect the cariogenicity of S. mutans . Conclusions AS vicK regulates vicK at the transcriptional and post-transcriptional levels, effectively inhibits EPS synthesis and biofilm formation and reduces its cariogenicity in vivo .

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“…This gene also regulates bacterial adhesion to the tooth surface, aggregation and coaggregation of the bacterial cells, and the integrity and stability of the biofilm structure. Therefore, its suppression may be associated with reduced biofilm/plaque formation and, ultimately, caries control [4,5]. We employed qRT-PCR to examine the expression of gtfB in S. mutans.…”

Section: Discussionmentioning

confidence: 99%

“…Dental biofilm, or plaque, constitutes a favorable environment for the growth of many bacteria, among which Streptococcus mutans (S. mutans) are known to play an important role in developing dental caries. In addition to acidic metabolites, S. mutans produces GtfB, GtfC, and GtfD, which are glucosyltransferase (GTF) enzymes that synthesize intracellular and extracellular polysaccharides [3,4]. They are encoded by gtfB, gtfC, and gtfD genes, respectively.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Effects of Postbiotic Mediators Derived from Lactobacillus rhamnosus GG and Lactobacillus reuteri on Streptococcus mutans

Banakar¹,

Pourhajibagher²,

Etemad‐Moghadam³

et al. 2023

Front. Biosci. (Landmark Ed)

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Background: Streptococcus mutans is a major component of dental plaque, contributing to cariogenic biofilm formation and inducing dental caries. Attempts have recently been made to use postbiotic mediators (PMs) to prevent dental caries. This research evaluated the antimicrobial/antibiofilm activity of PMs derived from Lactobacillus rhamnosus GG (LGG) and Lactobacillus reuteri (LR) against S. mutans in vitro. Methods: PMs were obtained from the Lactobacilli supernatants. The minimum inhibitory concentration, minimum bactericidal concentration, antibiofilm potential, and metabolic activity of PMs against S. mutans were evaluated using CFU/mL, scanning electron microscopy, and XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) reduction assay. The expression of gtfB gene as one of the most important genes involved in S. mutans biofilm formation was also measured using qRT-PCR. Results: CFU score was reduced by both PMs, but the reduction was only significant in LGG (p = 0.02). Both PMs caused a significant decrease in the metabolic activity of S. mutans compared with the controls (p ≤ 0.002). S. mutans treated with LGG PMs exhibited more destructive effects than LR PMs (p > 0.05). S. mutans gtfB gene expression was significantly downregulated when treated with the PMs obtained from both LGG and LR (p = 0.01 for both). Conclusions: We showed that PMs isolated from two Lactobacillus strains inhibited S. mutans biofilm, metabolic activity, and gtfB gene expression. Therefore, these derivatives may be a suitable biofilm-destruction agent against S. mutants. However, the oral environment is a complex ecosystem that needs further investigation.

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Lactobacillus reuteri BM53-1 Produces a Compound That Inhibits Sticky Glucan Synthesis by Streptococcus mutans

Cited by 10 publications

References 56 publications

Lactobacilli as Anti-biofilm Strategy in Oral Infectious Diseases: A Mini-Review

Lactobacilli as Anti-biofilm Strategy in Oral Infectious Diseases: A Mini-Review

Exopolysaccharides metabolism and cariogenesis of Streptococcus mutans biofilm regulated by antisense vicK RNA

Antimicrobial Effects of Postbiotic Mediators Derived from Lactobacillus rhamnosus GG and Lactobacillus reuteri on Streptococcus mutans

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