Lactobacillus rhamnosus GG ameliorates DON-induced intestinal damage depending on the enrichment of beneficial bacteria in weaned piglets

Abstract

Background Deoxynivalenol (DON) is one of the most common environmental pollutants that induces intestinal inflammation and microbiota dysbiosis. Lactobacillus rhamnosus GG (LGG) is a probiotic that not only has anti-inflammatory effects, but also shows protective effect on the intestinal barrier. However, it is still unknown whether LGG exerts beneficial effects against DON-induced intestinal damage in piglets. In this work, a total of 36 weaned piglets were randomized to one of four treatment groups for 21 d. The treatment groups were CON (basal diet); LGG (basal diet supplemented with 1.77 × 1011 CFU/kg LGG); DON (DON-contaminated diet) and LGG + DON (DON-contaminated diet supplemented with 1.77 × 1011 CFU/kg LGG). Result Supplementation of LGG can enhance growth performance of piglets exposed to DON by improving intestinal barrier function. LGG has a mitigating effect on intestinal inflammation induced by DON exposure, largely through repression of the TLR4/NF-κB signaling pathway. Furthermore, supplementation of LGG increased the relative abundances of beneficial bacteria (e.g., Collinsella, Lactobacillus, Ruminococcus_torques_group and Anaerofustis), and decreased the relative abundances of harmful bacteria (e.g., Parabacteroides and Ruminiclostridium_6), and also promoted the production of SCFAs. Conclusions LGG ameliorates DON-induced intestinal damage, which may provide theoretical support for the application of LGG to alleviate the adverse effects induced by DON exposure.

Black Lycium barbarum polysaccharide attenuates LPS-induced intestine damage via regulation gut microbiota

“…Previous study found increased Odoribacter in fecal microbiota transplantation-treated colitis mice ( Zhang et al, 2020 ), which was in line with LPS-induced mice, and higher concentration treatment of BLBP depicted a higher abundance of Odoribacter in mice in group GE ( p < 0.01). Lactobacillus is a commonly recognized genus of probiotics, which can maintain the intestinal barrier and provide protection against inflammation ( Bai et al, 2022 ), and a lower abundance of Lactobacillus had been found in antibiotic-associated diarrhea in mice ( Chen C. et al, 2022 ) and ulcerative colitis mice ( Hu et al, 2022 ). In the current study, a higher abundance of Lactobacillus was detected in mice in all BLBP-supplemented groups, especially in group GC and GE, which was in accordance with Lycium barbarum arabinogalactan treated mice with colitis ( Cao et al, 2022 ).…”

Comparison between Lactobacillus rhamnosus GG and LuxS-deficient strain in regulating gut barrier function and inflammation in early-weaned piglets

“…Inflammatory factors can bind to the membrane receptor TLR4, then trigger the TLR4/NF-κB signaling pathway to secrete a large number of inflammatory cytokines, and eventually lead to tissue damage ( 46 ). LGG ameliorated DON- and LPS-induced inflammation by modulating the TLR4/NF-κB signaling pathway ( 47 , 48 ). A recent report showed a new soluble protein from LGG, HM0539, suppress inflammation by inhibiting the TLR4/MyD88/NF-κB signaling, which is considered as a potential therapeutic option for inflammatory bowel disease (IBD) ( 49 ).…”

Deoxynivalenol: Emerging Toxic Mechanisms and Control Strategies, Current and Future Perspectives

“…20 The TLR4/NF-κB signaling pathway was activated in DON-induced intestinal toxicity and hepatotoxicity, and LGG can inhibit the TLR4/ NF-κB signaling pathway to alleviate intestinal inflammation and liver damage. 81,82 4.4. Notch 1.…”