Lactobacillus Sps in Reducing the Risk of Diabetes in High-Fat Diet-Induced Diabetic Mice by Modulating the Gut Microbiome and Inhibiting Key Digestive Enzymes Associated with Diabetes

Abstract

Obesity caused by a high-fat diet (HFD) affects gut microbiota linked to the risk of type-2 diabetes (T2D). This study evaluates live cells and ethanolic extract (SEL) of Lactobacillus sakei Probio65 and Lactobacillus plantarum Probio-093 as natural anti-diabetic compounds. In-vitro anti-diabetic effects were determined based on the inhibition of α-glucosidase and α-amylase enzymes. The SEL of Probio65 and Probio-093 significantly retarded α-glucosidase and α-amylase enzymes (p < 0.05). Live Probio65 and Probio-093 inhibited α-glucosidase and α-amylase, respectively (p < 0.05). In mice fed with a 45% kcal high-fat diet (HFD), the SEL and live cells of both strains reduced body weight significantly compared to HFD control (p < 0.05). Probio-093 also improved blood glucose level compared to control (p < 0.05). The gut microbiota modulatory effects of lactobacilli on HFD-induced diabetic mice were analyzed with qPCR method. The SEL and live cells of both strains reduced phyla Deferribacteres compared to HFD control (p < 0.05). The SEL and live cells of Probio-093 promoted more Actinobacteria (phyla), Bifidobacterium, and Prevotella (genus) compared to control (p < 0.05). Both strains exerted metabolic-modulatory effects, with strain Probio-093 showing more prominent alteration in gut microbiota, substantiating the role of probiotics in gut microbiome modulations and anti-diabetic effect. Both lactobacilli are potential candidates to lessen obesity-linked T2D.

Gut microbiota and renin-angiotensin system: a complex interplay at local and systemic levels

“…Antibiotic treatment in diabetic rats markedly lowered plasma acetate, inhibited intrarenal RAS activity, and reduced kidney damage. Also, several probiotic strains have been found to exert antidiabetic modulatory effects, i.e., by inhibiting enzymes that increase glucose absorption in the intestines ( 71 ) and by regulating postprandial blood glucose ( 72 ). In this regard, it is worth noting that alterations in GI RAS activity induced by diabetes disturb intestinal glucose transport and thereby contribute to postprandial hyperglycemia ( 73 ).…”

Evaluation of Probiotic and Antidiabetic Attributes of Lactobacillus Strains Isolated From Fermented Beetroot

“…According to recent scientific studies, supplementing meals with fermented foods can help to reduce the health risks connected with DM (Srihari et al, 2013 ; Zulkawi et al, 2018 ). The LAB isolated from fermented food sources is known for its gut microbiome modulations, decreasing low-grade inflammation, reducing glucose in the bloodstream, and demonstrating antidiabetic effects (Gulnaz et al, 2021 ; Youn et al, 2021 ). Beetroot ( Beta vulgaris L. sub sp.…”

Microbiota and body weight control: Weight watchers within?

“…Several probiotics effectively reduce body weight gain and adiposity in individuals with obesity. Specific strains or combinations of strains such as Streptococcus thermophilus , Lactobacillus acidophilus , Bifidobacterium lactis , B. animalislactis , L. gasseri , L. curvatus , Lactobacillus rhamnosus , Lactobacillus plantarum, L. fermentum, B. adolescentis , L. sakei , B. bifidum , B. infantis , B. breve , Enterococcus faecalis , L. parakefir , L. kefir , B. brevis , B. casei, Bacteroides vulgatus, Bacteroides dorei and Dysosmobacter welbionis have been detailed elsewhere to be effective for weight loss during obesity in humans and/or rodents [ 139 , 279 , [348] , [349] , [350] , [351] , [352] , [353] , [354] , [355] , [356] ]. However, other probiotic strains such as B. longum , L. reuteri and L. salivarius cause a decrease in body weight and metabolic syndrome-related parameters in obese rodents but are mainly ineffective or partially effective in humans [ 349 , 357 ].…”