Lactobacillus strains belonging to Casei group display various adherence to enterocytes and mucus

Markowicz, Corinna; Schmidt, Marcin

doi:10.17306/j.afs.2015.3.26

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…In addition, the presence of mucus layer, as a protective barrier in the GI tract, plays a crucial role in the bacterial adhesion mechanism and several data literature report that some lactobacilli are not able to bind to the mucus layer ( Jonsson et al, 2001 ; Duary et al, 2011 ; Jensen et al, 2014 ). Moreover, lactobacilli showing high adhesion properties to intestinal cells do not always display the same capability to bind to the mucin ( Markowicz and Schmidt, 2015 ). Therefore, we investigated all the strains to assess the potential impact of mucin on adhesion efficiency and to identify their ability to grow in a medium containing mucin ( Van Tassell and Miller, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Adhesion Properties of Food-Associated Lactobacillus plantarum Strains on Human Intestinal Epithelial Cells and Modulation of IL-8 Release

et al. 2018

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Food-associated microbes can reach the gut as viable cells and interact with the human host providing potential health benefits. In this study, we evaluated the impact on cell viability and the adhesion ability of 22 Lactobacillus plantarum strains, mainly isolated from fermented foods, on a Normal-derived Colon Mucosa cell line. Furthermore, due to the presence of mucus layer on the gut epithelium, we also investigated whether the mucin could affect the microbial adhesion property. Our results demonstrated that all the strains displayed a strong ability to adhere to host cells, showing a strain-dependent behavior with preference for cell edges, that resulted not to be affected by the presence of mucin. Based on interleukin-8 release of intestinal cells induced by some Lb. plantarum strains, our data suggest a potential cross-talk with the host immune system as unconventional property of these food-associated microbes.

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Section: Discussionmentioning

confidence: 99%

Adhesion Properties of Food-Associated Lactobacillus plantarum Strains on Human Intestinal Epithelial Cells and Modulation of IL-8 Release

et al. 2018

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“…1), which differed from the adhesion potential towards Caco-2 intestinal epithelium cells and towards the intestinal mucus (Markowicz and Schmidt, 2015). Five of the tested strains -L. rhamnosus KL53A, L. casei HI2, L. rhamnosus HI5, L. rhamnosus GG and L. casei HI3, showed very good or good adhesion properties towards HeLa and Caco-2 cells as well as the intestinal mucus.…”

Section: Detection Of Gapdh and Tufm In Extracellular Protein Fractiomentioning

confidence: 94%

Lactobacillus spp. belonging to the Casei group display a variety of adhesins

Konieczna

Olejnik-Schmidt

Schmidt

2018

Acta Sci Pol Technol Aliment

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Introduction. Adhesion of bacteria from the genus Lactobacillus to the gastrointestinal epithelium is, to a considerable degree, dependent on the interactions between adhesins found on the surface of bacterial cells and elements found within the epithelium. A significant role in these interactions is played by bacterial proteins exposed to the cell wall surface, which are capable of binding to molecules of substances comprising the extracellular matrix of the intestinal epithelium. Materials and methods.In order to analyze the extracellular proteome of intestinal bacteria in terms of the presence of cell adhesion molecules, a total of twenty strains from the Lactobacillus spp. group Casei were tested. The analyses were conducted using SDS PAGE, 2-D electrophoresis, Western blot and mass spectrometry. An experiment was also conducted to assess the adhesion capacity of the tested strains to cervical epithelial cells (HeLa).Results. The tested strains varied in their adhesion efficiency to HeLa cells, ranging from 0.5% to 29%. Using electrophoretic methods a total of 54 extracellular protein fractions were distinguished in these strains, additionally identifying potential adhesion molecules (e.g. a surface antigen of the NLP/P60 family and a small heat shock protein/chaperonin). Discussion. The identification of these proteins in the extracellular proteome of Latobacillus spp. isolates may suggest that they serve currently unknown functions on the cell surface, including those connected with the interactions between bacteria and the intestinal epithelium. Such analyses may provide insight into new factors promoting probiotic adhesion to various types of epithelial cells.

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“…The tested strains showed varied adhesion potential towards cervical epithelial cells (Fig. 1), which differed from the adhesion potential towards Caco-2 intestinal epithelium cells and towards the intestinal mucus (Markowicz and Schmidt, 2015). Five of the tested strains -L. rhamnosus KL53A, L. casei HI2, L. rhamnosus HI5, L. rhamnosus GG and L. casei HI3, showed very good or good adhesion properties towards HeLa and Caco-2 cells as well as the intestinal mucus.…”

Section: Identification Of Potential Adhesins Using Mass Spectrometrymentioning

confidence: 99%

Lactobacillus spp. belonging to the Casei group display a variety of adhesins [pdf]

Konieczna¹,

Olejnik-Schmidt²,

Schmidt³

2018

Acta Sci Pol Technol Aliment

Self Cite

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Introduction. Adhesion of bacteria from the genus Lactobacillus to the gastrointestinal epithelium is, to a considerable degree, dependent on the interactions between adhesins found on the surface of bacterial cells and elements found within the epithelium. A significant role in these interactions is played by bacterial proteins exposed to the cell wall surface, which are capable of binding to molecules of substances comprising the extracellular matrix of the intestinal epithelium. Materials and methods. In order to analyze the extracellular proteome of intestinal bacteria in terms of the presence of cell adhesion molecules, a total of twenty strains from the Lactobacillus spp. group Casei were tested. The analyses were conducted using SDS PAGE, 2-D electrophoresis, Western blot and mass spectrometry. An experiment was also conducted to assess the adhesion capacity of the tested strains to cervical epithelial cells (HeLa). Results. The tested strains varied in their adhesion efficiency to HeLa cells, ranging from 0.5% to 29%. Using electrophoretic methods a total of 54 extracellular protein fractions were distinguished in these strains, additionally identifying potential adhesion molecules (e.g. a surface antigen of the NLP/P60 family and a small heat shock protein/chaperonin). Discussion. The identification of these proteins in the extracellular proteome of Latobacillus spp. isolates may suggest that they serve currently unknown functions on the cell surface, including those connected with the interactions between bacteria and the intestinal epithelium. Such analyses may provide insight into new factors promoting probiotic adhesion to various types of epithelial cells.

show abstract

Lactobacillus strains belonging to Casei group display various adherence to enterocytes and mucus

Cited by 3 publications

References 19 publications

Adhesion Properties of Food-Associated Lactobacillus plantarum Strains on Human Intestinal Epithelial Cells and Modulation of IL-8 Release

Adhesion Properties of Food-Associated Lactobacillus plantarum Strains on Human Intestinal Epithelial Cells and Modulation of IL-8 Release

Lactobacillus spp. belonging to the Casei group display a variety of adhesins

Lactobacillus spp. belonging to the Casei group display a variety of adhesins [pdf]

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