Abstract:Lactobionic acid was produced by acetic acid bacteria to oxidize lactose. Gluconobacter spp. and Gluconacetobacter spp. showed higher lactose-oxidizing activities than Acetobacter spp. Gluconobacter frateurii NBRC3285 produced the highest amount of lactobionic acid per cell, among the strains tested. This bacterium assimilated neither lactose nor lactobionic acid. At high lactose concentration (30%), resting cells of the bacterium showed sufficient oxidizing activity for efficient production of lactobionic aci… Show more
“… 21 The reported most efficient producer of cellobionic acid, Gluconobacter frateurii NBRC3285, produced cellobionic acid with a yield of only about 3 mM with the addition of 27.8 mM cellobiose to the culture medium. 10 Herein, apparent improvement of the cellobionic acid production efficiency (50 mg L −1 ) was achieved using P. frag with addition of 1 g L −1 cellobiose in the culture. Further upgrade of the above process should have industrial applications for cellobionic acid production which can be used as a substrate to produce other valuable biochemicals.…”
Aldonic acids are receiving increased interest due to their applications in nanotechnology, food, pharmaceutical and chemical industries. Microbes with aldose-oxidizing activity, rather than purified enzymes, are used for commercial production with limited success. Thus it is still very important to develop new processes using strains with more efficient and novel biocatalytic activities for the production of adonic acids. In the present study, Pseudomonas fragi TCCC11892 was found to be an efficient producer of aldonic acids, with the production of galactonic and L-rhamnonic acid by P. fragi reported for the first time. The semi-continuous production of maltobionic acid and lactobionic acid was developed for P. fragi TCCC11892, achieving a yield of over 90 g L À1 for the first 7 cycles. The excellent performance of P. fragi in the production of lactobionic acid (119 g L À1 ) was also observed when using waste cheese whey as an inexpensive fermentation medium. Scaling up of the above process for production of aldonic acids with P. fragi TCCC11892 cells should facilitate their commercial applications.
“… 21 The reported most efficient producer of cellobionic acid, Gluconobacter frateurii NBRC3285, produced cellobionic acid with a yield of only about 3 mM with the addition of 27.8 mM cellobiose to the culture medium. 10 Herein, apparent improvement of the cellobionic acid production efficiency (50 mg L −1 ) was achieved using P. frag with addition of 1 g L −1 cellobiose in the culture. Further upgrade of the above process should have industrial applications for cellobionic acid production which can be used as a substrate to produce other valuable biochemicals.…”
Aldonic acids are receiving increased interest due to their applications in nanotechnology, food, pharmaceutical and chemical industries. Microbes with aldose-oxidizing activity, rather than purified enzymes, are used for commercial production with limited success. Thus it is still very important to develop new processes using strains with more efficient and novel biocatalytic activities for the production of adonic acids. In the present study, Pseudomonas fragi TCCC11892 was found to be an efficient producer of aldonic acids, with the production of galactonic and L-rhamnonic acid by P. fragi reported for the first time. The semi-continuous production of maltobionic acid and lactobionic acid was developed for P. fragi TCCC11892, achieving a yield of over 90 g L À1 for the first 7 cycles. The excellent performance of P. fragi in the production of lactobionic acid (119 g L À1 ) was also observed when using waste cheese whey as an inexpensive fermentation medium. Scaling up of the above process for production of aldonic acids with P. fragi TCCC11892 cells should facilitate their commercial applications.
“… Fig. 3 Schematic representation of the reaction mechanism of the membrane-bound glucose dehydrogenase (mGDH), which is responsible for the formation of MBA or CBA in Pseudomonas species and acetic acid bacteria [ 1 , 3 , 95 – 97 , 101 – 105 ]. The pyrroloquinoline-quinone-dependent (PQQ) dehydrogenase is catalyzing the oxidation of maltose or cellobiose.…”
Section: Biological Production Methodsmentioning
confidence: 99%
“…One of the most efficient producers of CBA has been Gluconobacter frateurii NBRC3285 [ 101 ] . The CBA concentration achieved with this bacterium in a whole-cell biocatalysis was 0.86 g L −1 , which corresponded to a yield of 9% and a volumetric productivity of 0.04 g L −1 h −1 .…”
Aldobionic acids are sugar acids which consist of a disaccharide with an anomeric acid group. The most famous is lactobionic acid (LBA). LBA is used in many applications such as food and beverages, pharmaceuticals and medicine, cosmetics or chemical processes. During the last decade, all these industries are observing a shift of consumer preferences towards plant-based options. Thus, the biotechnological industry is trying to replace the animal-derived LBA. Maltobionic acid (MBA) and cellobionic acid (CBA) are two stereoisomers of LBA which have emerged as vegan alternatives. However, MBA and CBA face different obstacles related to their industrial production. While traditionally used electrochemical or chemical catalysis often rely on cost intensive and/or hazardous catalysts, novel production methods with microorganisms are still poorly studied. In the first part, this paper discusses both alternatives in terms of their characteristics and applications. In the second part, it reviews the long-studied chemical production and the novel bioproduction methods, which are based on enzymatic and microbial systems. This review concludes with a discussion of future work needed to bring their production to the industrial scale.
“…Similarly, LBA can also be prepared by oxidation of the free aldehyde group of lactose through the application of strains such as Escherichia coli [2,17], Pseudomonas fragi [18], Zymomonas mobilis [19], Gluconobacter spp., and Gluconacetobacter spp. [20]. However, these methods imply procedures that involve more time and equipment for the LBA preparation process.…”
Lactobionic acid (LBA) is a polyhydroxy acid with attractive properties in the pharmaceutical, cosmetic, food, medical, and chemical industries, making it a versatile product with multiple applications, which supports the various studies aimed at its production by increasingly more simple, efficient, and environmentally friendly processes. For this reason, the purpose of this research was to synthesize gold nanoparticles (AuNPs) by a synthesis process using Myrciaria dubia (Camu camu) fruit extract. Subsequently, AuNPs were used to produce LBA from lactose. The results demonstrate that the Myrciaria dubia extract manages to synthesize AuNPs that were characterized by UV/vis spectrophotometry, energy-dispersive X-ray spectroscopy (EDX), and Zetasizer. LBA was quantified by FTIR-ATR spectroscopy and ion chromatography. The results showed that AuNPs succeeded in producing LBA from lactose showing the highest LBA production efficiency at a dose of 0.5 g/L and a temperature of 60°C. It has been shown that the AuNPs obtained by synthesis using the Myrciaria dubia extract efficiently catalyze the production of LBA from lactose, with a yield of 45.24%, which can be used to produce LBA for industrial or research purposes.
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