Efficient production of sugar-derived aldonic acids byPseudomonas fragiTCCC11892

Abstract

Aldonic acids are receiving increased interest due to their applications in nanotechnology, food, pharmaceutical and chemical industries. Microbes with aldose-oxidizing activity, rather than purified enzymes, are used for commercial production with limited success. Thus it is still very important to develop new processes using strains with more efficient and novel biocatalytic activities for the production of adonic acids. In the present study, Pseudomonas fragi TCCC11892 was found to be an efficient producer of aldonic acids, with the production of galactonic and L-rhamnonic acid by P. fragi reported for the first time. The semi-continuous production of maltobionic acid and lactobionic acid was developed for P. fragi TCCC11892, achieving a yield of over 90 g L À1 for the first 7 cycles. The excellent performance of P. fragi in the production of lactobionic acid (119 g L À1 ) was also observed when using waste cheese whey as an inexpensive fermentation medium. Scaling up of the above process for production of aldonic acids with P. fragi TCCC11892 cells should facilitate their commercial applications.

High-level production of maltobionic acid from high-maltose corn syrup by genetically engineered Pseudomonas taetrolens

“…7 ). These results surpassed those of previous findings of microorganisms as MBA producers [ 14 , 15 ].

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“…Here, we aimed to enhance MBA production by optimizing the substrate concentration, growth temperature, aeration, and cell density of seed cultures using a genetically modified P. taetrolens strain, which homologously expresses the quinoprotein GDH. To our knowledge, the MBA titer, yield, and productivity from pure maltose or high-maltose corn syrup (HMCS) substrates were higher than those of a previous study of applying microorganisms to produce MBA [ 14 , 15 ] and comparable to those obtained in microbial LBA production [ 20 ].…”

“…To date, two reports have described microbial MBA production by Pseudomonas sp. and Pseudomonas fragi , but the production was much lower (94.7 g/L) than that of LBA (400 g/L) [ [14] , [15] , [16] ].…”

Valorization of Gelidium amansii for dual production of D-galactonic acid and 5-hydroxymethyl-2-furancarboxylic acid by chemo-biological approach

“…In terms of oxidizing D-galactose and HMF into D-galactonic acid and HMFCA, it is desirable to explore strains that possess strong and specific activity towards the aldehyde group. As typically aerobic bacteria, the genus Pseudomonas can oxidize a wide range of aldose sugars into their corresponding aldonic acids [13,14], but their potential capacities of HMF oxidation have not been fully investigated. In this study, five different species from Pseudomonas sp.…”

“…Up to now, strains screened and applied for D-galactonic acid and HMFCA production are relatively few and mainly concentrated in some aerobic bacteria. Four different species of Pseudomonas were tested for their aldose-oxidizing activities and P. fragi TCCC 11892 was found to be an efficient producer of aldonic acids, including D-galactonic acid [14]. G. oxydans was another versatile species that can convert a series of biomass-derived sugars into corresponding aldonic acids [9,33,34].…”