Lactoferrin nanoparticle mediated targeted delivery of 5-fluorouracil for enhanced therapeutic efficacy

Kumari, Sonali; Kondapi, Anand K.

doi:10.1016/j.ijbiomac.2016.10.110

Cited by 46 publications

(21 citation statements)

References 21 publications

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“…Further, the FT-IR spectra suggest the stability of TMZ and Lf protein in the nanoformulation, as the characteristic peaks corresponding to TMZ and Lf are observed in the nanoformulation as well. Fourthly, LfNPs show a surprising ability to release TMZ (4-fold excess) at acidic pH, a phenomenon observed for several other drugs by our group 21, 24 . This property may facilitate a triggered TMZ release within the acidic tumor interstitium and in the endosomal compartments, following LfR-mediated endocytosis of LfNPs.…”

Section: Discussionsupporting

confidence: 52%

“…TMZ-LfNPs were prepared by a previously established sol-oil method 24, 25 . The TMZ-LfNPs, as characterized by TEM and DLS, showed a spherical morphology and uniform size distribution in the range of 70 ± 10 nm (TEM) ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The ability of Lf to cross BBB and its application in drug/gene delivery has been previously exploited through functionalization of nanoparticles with Lf 31–34 . However, for the first time, utilization of Lf as a sole matrix for developing nano-formulations was reported by our group 21, 24, 25 . In the present study, TMZ loaded Lf nanoparticles were developed and their brain targeting abilities were evaluated which till now has remained unexplored.…”

Section: Discussionmentioning

confidence: 97%

“…Compared to other methods of protein nanoparticle preparation, viz . coacervation 42 , salt-precipitation, heat denaturation 43 etc, our method is non-disruptive and maintains the physiological conformation of the Lf protein thereby preserving its receptor binding properties 24 . Also, developed from a natural protein (Lf), LfNPs were found to be completely safe even at high doses.…”

Section: Discussionmentioning

confidence: 99%

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Overcoming blood brain barrier with a dual purpose Temozolomide loaded Lactoferrin nanoparticles for combating glioma (SERP-17-12433)

Kumari

Ahsan

Kumar

et al. 2017

Sci Rep

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122

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Targeted delivery of drugs to the brain is challenging due to the restricted permeability across the blood brain barrier (BBB). Gliomas are devastating cancers and their positive treatment outcome using Temozolomide (TMZ) is limited due to its short plasma half-life, systemic toxicity and limited access through the blood-brain barrier (BBB). Nanoparticles made of Lactoferrin (Lf) protein, have been shown to enhance the pharmacological properties of drugs. Here, we report the specific ability of Lf nanoparticles to cross BBB and target over-expressed Lf receptors on glioma for enhanced TMZ delivery. TMZ-loaded Lf nanoparticles (TMZ-LfNPs) were prepared by our previously reported sol-oil method. While the Lf protein in the NP matrix aids in transcytosis across the BBB and preferential tumor cell uptake, the pH responsiveness leads to TMZ release exclusively in the tumor microenvironment. Delivery through LfNPs results in an enhanced and sustained intracellular concentration of TMZ in GL261 cells in vitro along with improving its in vivo pharmacokinetics and brain accumulation. TMZ-LfNPs treatment results in a significant reduction of tumor volume, higher tumor cell apoptosis and improved median survival in glioma bearing mice. These results demonstrate that LfNPs present an efficient TMZ delivery platform for an effective treatment of gliomas.

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Section: Discussionsupporting

confidence: 52%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Overcoming blood brain barrier with a dual purpose Temozolomide loaded Lactoferrin nanoparticles for combating glioma (SERP-17-12433)

Kumari

Ahsan

Kumar

et al. 2017

Sci Rep

Self Cite

122

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“…Some examples of antimicrobial peptides from animal origin can be used as food additives such as: lactoferrin [21], bovine casein peptides [22], chitosan [23], and pleurodynia [24].…”

Section: Natural Preservativesmentioning

confidence: 99%

Can Microalgae Act as Source of Preservatives in Food Chain?

Scaglioni¹,

Badiale‐Furlong²

2017

JFSE

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Microalgae are a potential source for various valuable chemicals for commercial applications ranging from nutraceuticals to fuels. This review aims to focus on microalgal as preservative potential to food chain. The main compounds responsible for these properties, like carotenoids, phenolic compounds, antioxidant vitamins, sterols and polyunsaturated fatty acids are discussed because microalgae can be cultivated under controlled conditions to improve their production. We have listed several studies performed in recent years supporting the health benefits that most of these compounds can provide. Therefore, they may represent an excellent source of preservatives in food chain once their safety as a food supplement has been also confirmed.

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