Lactosylated Chitosan for DNA Delivery into Hepatocytes:  The Effect of Lactosylation on the Physicochemical Properties and Intracellular Trafficking of pDNA/Chitosan Complexes

Hashimoto, Mayu; Morimoto, Minoru; Saimoto, Hiroyuki; Shigemasa, Yoshihiro; Sato, Toshinori

doi:10.1021/bc050228h

Cited by 116 publications

(89 citation statements)

References 37 publications

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“…A significant increase in turbidity was observed after the incubation of Ch-DNA polyplexes with 2 mg/mL BSA. However, lactosylated-Ch based complexes were stable even after the incubation for 24 h [49].…”

Section: Colloidal Stability In Physiological Conditionsmentioning

confidence: 95%

Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside

et al. 2014

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Non-viral gene delivery vectors are emerging as a safer alternative to viral vectors. Among natural polymers, chitosan (Ch) is the most studied one, and low molecular weight Ch, specifically, presents a wide range of advantages for non-viral pDNA delivery. It is crucial to determine the best process for the formation of Low Molecular Weight Chitosan (LMWC)-pDNA complexes and to characterize their physicochemical properties to better understand their behavior once the polyplexes are administered. The transfection efficiency of Ch based polyplexes is relatively low. Therefore, it is essential to understand all the transfection process, including the cellular uptake, endosomal escape and nuclear import, together with the parameters involved in the process to improve the design and development of the non-viral vectors. The aim of this review is to describe the formation and characterization of LMWC based polyplexes, the in vitro transfection process and finally, the in vivo applications of LMWC based polyplexes for gene therapy purposes.

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Section: Colloidal Stability In Physiological Conditionsmentioning

confidence: 95%

Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside

et al. 2014

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“…Many research studies have been conducted for enhancement of transfection efficiency, such as pHsensitive modification , Jones, et al, 2003, Kim, et al, 2003, Wong, et al, 2006, temperature-sensitive modification , Dang, et al, 2006, Sun, et al, 2005, specific target ligand modification (Hashimoto, et al, 2006, Mansouri, et al, 2006, Wu & Wu, 1998 and so on. Among the chemical modifications of chitosan, PEI grafted chitosan showed some benefit due to high transfection efficiency.…”

Section: Cyclodextrinmentioning

confidence: 99%

Medical Polymer-Based Gene Therapy

Jiang¹,

Kim²,

Cho³

et al. 2011

Non-Viral Gene Therapy

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“…Destabilization has been proposed for the escape of poly-L-histidine, PLL, PLGA, PAMAM dendrimers, PEI, and chitosan. 10,21,24,71,81,82 During acidification, polymers scavenge protons and become increasingly cationic. In a mechanism similar to that observed with lipoplexes, highly cationic polyplexes destabilize the endosomal membrane bilayer through phospholipid flipping, creating imperfections that allow complex release (Figure 3).…”

Section: Membrane Destabilizationmentioning

confidence: 99%

Toward Development of Artificial Viruses for Gene Therapy: A Comparative Evaluation of Viral and Non‐viral Transfection

Douglas¹

2008

Biotechnology Progress

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Problems related to the safety and efficacy of gene therapies have checked the enthusiasm once surrounding this field, though it remains a promising approach for the treatment of numerous diseases. Despite the high transfection efficiencies attainable using viral vectors, manufacturing difficulties, safety concerns, and limitations related to targeting and plasmid size have prompted considerable research into the development of non‐viral vectors. Non‐viral vectors demonstrate low toxicity, low immunogenicity, and ease of manufacture. However, they have not yet achieved the transfection efficiencies displayed by viruses. The inability to explain or predict transfection efficiencies results, in part, from insufficient understanding of the intracellular processes involved in gene delivery. Increasingly, research has been undertaken to probe the processes involved in overcoming the major obstacles to vector‐mediated transfection: (1) internalization, (2) intracellular trafficking, (3) escape to the cytosol, (4) nuclear translocation, and (5) gene transcription/expression. This paper reviews and compares the pathways and techniques involved in successful viral and non‐viral transfection. In addition, this review provides evidence that non‐viral vector development has been pursued successfully thus far, producing systems capable of evading almost all major obstacles to transfection. Evaluating the abilities of non‐viral and viral vectors to overcome specific cellular barriers reveals that the greatest advantage of viral vectors may be related to viral DNA, which is transcribed considerably more efficiently than plasmid DNA. Further study in this area should enable the development of non‐viral vectors that transfect as efficiently as viral vectors.

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Lactosylated Chitosan for DNA Delivery into Hepatocytes: The Effect of Lactosylation on the Physicochemical Properties and Intracellular Trafficking of pDNA/Chitosan Complexes

Cited by 116 publications

References 37 publications

Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside

Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside

Medical Polymer-Based Gene Therapy

Toward Development of Artificial Viruses for Gene Therapy: A Comparative Evaluation of Viral and Non‐viral Transfection

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