Lactuca sativa biofertilization using biofilm from  Bacillus  with  PGPR activity

Galelli, Mirta Esther; Sarti, Gabriela Cristina; Miyazaki, Silvia Susana

doi:10.37855/jah.2015.v17i03.35

Cited by 13 publications

(6 citation statements)

References 18 publications

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“…in promoting the growth of different crops. Galelli et al (2015) found an increase of 64 % in the weight of the shoots and 68 % for the roots in lettuce (Lactuca sativa) inoculated with a PGPR Bacillus subtillis (planktonic form). Furthermore, the authors tested the effect of biofilms as a strategy to increase the contact between the bacteria and the plant and found an increase in growth for the shoots of 39 % and 59 % for the roots.…”

Section: Discussionmentioning

confidence: 98%

Isolation and selection of endophytic spore-forming bacteria with plant growth promoting properties isolated from Ilex paraguariensis St. Hil. (yerba mate)

Laczeski

Onetto

Cortese

et al. 2020

An. Acad. Bras. Ciênc.

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Yerba mate (Ilex paraguariensis St. Hil.) is a species native to the subtropical regions of South America. Despite being an important crop for the region, there are few studies on the use of microorganisms to improve the growth of seedlings in the nursery stage. The objective of this study was to isolate spore-forming endophytic bacteria with plant growth promoting properties associated with yerba mate seedlings and determine their phytobeneficial effect under controlled laboratory conditions. Isolates were selected based on their sporulation capacity and evaluated for in vitro plant growth promoting properties (nitrogen fixation, phosphate solubilization, production of siderophores and synthesis of indolic compounds). Yerba mate seedlings were inoculated with the most promising isolates, which were identified via analyses of the sequence of their 16S rDNA gene as Bacillus circulans (12RS3) and Bacillus altitudinis (19RS3, T5S-T4). After 120 days plants showed higher root dry weight when inoculated with isolate 19RS3 and higher shoot dry weight with 19RS3 and T5S-T4. In conclusion, further studies to determine the ability of these isolates to adapt to the climatic conditions and to survive amidst the native soil microflora in yerba mate cultivated native soils, will be crucial for developing such strains as biofertilizer.

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Section: Discussionmentioning

confidence: 98%

Isolation and selection of endophytic spore-forming bacteria with plant growth promoting properties isolated from Ilex paraguariensis St. Hil. (yerba mate)

Laczeski

Onetto

Cortese

et al. 2020

An. Acad. Bras. Ciênc.

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“…Most bio-inoculants grow as bacterial vegetative forms, i.e., as free-living planktonic bacteria; however, some of them, including B. subtilis subsp. spizizenii, have been shown to be able to grow both in the planktonic form or as a biofilm [27,51]. Species that grow predominantly as biofilms, such as B. subtilis, are thought to possess advantages in terms of reproducing and surviving in the environment [52].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, B. subtilis subsp. spizizenii can either develop a biofilm attached to a substrate or a surface or live as a free planktonic form depending on the cultivation environment [27,28]. Subsequently, this PGPR can be inoculated both as a biofilm or as a liquid in the conventional planktonic mode.…”

Section: Introductionmentioning

confidence: 99%

Inoculation with Biofilm of Bacillus subtilis Is a Safe and Sustainable Alternative to Promote Tomato (Solanum lycopersicum) Growth

Sarti,

Galelli,

Cristóbal-Miguez

et al. 2024

Environments

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Over-fertilization and agrochemicals adversely affect soil quality and agricultural ecosystem sustainability. Tomato (Solanum lycopersicum) is ranked as an important crop due to its high profitability and nutritional value. In Argentina, tomato is mainly produced in horticultural belts at peri-urban areas, whose soils frequently are contaminated by heavy metals and/or agrochemicals. To explore safer alternatives, we investigated the effects of seed inoculation with a common plant-growth-promoting rhizobacteria (PGPR), i.e., Bacillus subtilis subsp. spizizenii, on development at various growth stages of two tomato varieties, “Platense” and “Río Grande”, and on production and fruit quality at harvest time of the “Río Grande” variety. The experimental design consisted of three treatments per variety: a control versus traditional planktonic or biofilm inoculation, with three replicates per treatment. Germination at 10 days and seedling agronomical parameters showed that the response to seed inoculation was superior in the “Río Grande” variety. At harvest time, and irrespective of the inoculant, several agronomical parameters of the “Río Grande” variety were significantly enhanced with respect to the control. The biofilm significantly increased tomato production, as quantified by fruit number and weight, compared to the planktonic inoculum. This case study demonstrates that the incorporation of bio-inoculants is relevant in sustainable agriculture to promote crop growth and quality.

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“…In this sense, B. subtilis subsp. spizizenii, can form a biofilm [42], a characteristic that differentiates it from other microorganisms used as a bioinoculant. The cells of the biofilm are held together by an extracellular matrix composed of exopolysaccharides, proteins, and nucleic acids [43].…”

Section: Discussionmentioning

confidence: 99%

Inoculation with Biofilm of <em>Bacillus subtilis</em> Promotes the Growth of <em>Lactuca sativa</em>

Sarti,

Galelli,

Arreghini

et al. 2023

Preprint

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In Argentina there are urban areas of intensive cultivation around most of the big cities, where the soils may be unsuitable for some of the horticultural species and the use of chemical fertilizers is common. Bioinoculants based on plant growth promoting rhizobacteria (PGPR) could be an al-ternative for crop improvement, without affecting the environment. These formulations are gen-erally liquid, and their main disadvantage is the low viability of microorganisms. The objectives of this work were to evaluate a different inoculation method, the application of the biofilm pro-duced by Bacillus subtilis as a growth promoter on seeds of three varieties of Lactuca sativa. Biofilm was obtained under static culture conditions, the liquid (planktonic) inoculum was produced at 150 rpm. The major effects were observed with Bacillus subtilis subsp. spizizenii, that showed an-tifungal activity against phytopathogens, synthesize plant growth regulators (IAA, cytokinin and ABA) and solubilize phosphates. The best results were obtained with the Grand Rapid variety inoculated with biofilm, its positive effect was throughout the plant development until the har-vest time, when the bacterium was recovered from the interior of the roots. The biofilm of B. sub-tilis subsp. spizizenii would be an innovative and promising seed inoculant for horticultural spe-cies.

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Lactuca sativa biofertilization using biofilm from Bacillus with PGPR activity

Cited by 13 publications

References 18 publications

Isolation and selection of endophytic spore-forming bacteria with plant growth promoting properties isolated from Ilex paraguariensis St. Hil. (yerba mate)

Isolation and selection of endophytic spore-forming bacteria with plant growth promoting properties isolated from Ilex paraguariensis St. Hil. (yerba mate)

Inoculation with Biofilm of Bacillus subtilis Is a Safe and Sustainable Alternative to Promote Tomato (Solanum lycopersicum) Growth

Inoculation with Biofilm of <em>Bacillus subtilis</em> Promotes the Growth of <em>Lactuca sativa</em>

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