This thesis studies the turbulent energy cascade from the perspective of statistical mechanics and relates inter-scale energy fluxes to information-entropy production. The microscopical reversibility of the energy cascade is tested by constructing a reversible 3D turbulent system using a dynamic model for the sub-grid stresses. This system, when reversed in time, develops a sustained inverse cascade towards the large scales, evidencing that the characterization of the inertial energy cascade must consider the possibility of an inverse regime. This experiment suggests the introduction of a probabilistic concept, namely entropy, to explain the prevalence of direct over inverse energy cascades. Entropy production, as a statistical property of ensembles in phase space, is connected to the dynamics of the energy cascade in physical space by considering the space locality of the energy fluxes and their relation to the local structure of the flow. An entropic mechanism for the prevalence of direct energy transfer is proposed based on the dynamics of the rate-of-strain tensor, which is identified as an important indicator of statistical irreversibility in the energy cascade. A deeper analysis of the entropy generation mechanisms is accomplished by defining a space-local measure of phase-space mixing based on the Lyapunov exponents. The statistics of this quantity consistently reveal that the dynamics of the rate-of-strain tensor are fundamentally connected to entropy production. This analysis, which also describes the spatio-temporal structure of chaos in the energy cascade, reveals a strong localization of highly chaotic and entropy-producing events in the vicinity of interacting vortical structures. I would like to thank my family, specially Nadia, friends, and work colleagues for their support and encouragement during the final stages of this thesis. I thank Lindy Hop for making these years probably one of the happiest times in my life, and for not letting me take anything too seriously. I would like to thank my advisor, Javier Jiménez, for choosing a beautiful topic that has allowed me to get in touch with the fascinating field of non-equilibrium thermodynamics. I also thank him for passive and actively fostering my independence.