Lamb Fetal Pulmonary Fluid. II. Fate of Phosphatidylcholine

Scarpelli, Emile M.; Condorelli, S; Colacicco, Giuséppe; Cosmi, E. V.

doi:10.1203/00006450-197504000-00011

Cited by 27 publications

(9 citation statements)

References 2 publications

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“…Limited data reported in fetal sheep at term suggested that labeled phosphatidylcholine appeared in the surfactant more slowly in fetal than in 3 -8-day-old lambs 120). Further, radiolabeled phosphatidylcholine bound to albumin has been reported to be cleared from the tracheal fluid of term lamb fetuses with a half-life of 15 -57 min [21), a value very different than the 54 hr reported here. In the present study, the labeling curves for saturated phosphatidylcholine in newborn and adult animals were similar.…”

Section: Discussioncontrasting

confidence: 62%

The Labeling of Pulmonary Surfactant Phosphatidylcholine in Newborn and Adult Sheep

Ikegami

Jobe

Nathanielsz

1981

Experimental Lung Research

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The labeling of the saturated phosphatidylcholine from surfactant with radiolabeled palmitic acid was characterized in seven newborn and seven adult sheep using a repetitive sampling technique. Each animal had a small cannula placed surgically in the trachea. Following the intravenous injection of (3H) palmitic acid, surfactant samples in saline were recovered from the distal airways of each animal with fine plastic catheters over a period of 10 days. The change in specific activity of the saturated phosphatidylcholine (cpm/mumol) was used to define the kinetics of secretion and then disappearance of the labeled saturated phosphatidylcholine. Labeled saturated phosphatidylcholine accumulated in a linear fashion without an apparent initial delay for 27 hr in adult and 44 hr in newborn sheep. The labeled saturated phosphatidylcholine then decayed with mean apparent biological half-life values of 45 hr and 54 hr in adult and newborn sheep, respectively. However, these half-life estimates are compromised by the long secretory phase of the labeling curves. The characteristics of the labeling of surfactant saturated phosphatidylcholine in sheep may be more representative of surfactant metabolism in large mammals than previous studies in small rodents.

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Section: Discussioncontrasting

confidence: 62%

The Labeling of Pulmonary Surfactant Phosphatidylcholine in Newborn and Adult Sheep

Ikegami

Jobe

Nathanielsz

1981

Experimental Lung Research

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“…It is, therefore, likely that the clearance of materials occurring in the alveolar space, at least of alveolar phospholipids, may be impaired in this disease. Several mechanisms have been proposed to explain how pulmonary surfactant is removed from the alveolar space: (a) Clearance via the air way (Gluck et al 1971), (b) Phagocytosis by alveolar macrophages (Naimark 1973), (c) Transportation through intra-alveolar septa to the lymphatics (Bensch et al 1967), and (d) Re-entry into the alveolar epithelium where it might be reutilized (Scarpelli et al 1971). It is unknown which mechanism is impaired in this disease.…”

Section: Protein Analysismentioning

confidence: 99%

Biochemical characterization of pulmonary washings of patients with alveolar proteinosis, interstitial pneumonitis and alveolar cell carcinoma.

Onodera

Nakamura

Sato

et al. 1983

Tohoku J. Exp. Med.

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“…The rate at which FPF is formed is about 1.5 ml/hr.kg; however, when the fetus breathed in utero, FPF volume decreased rapidly and after the fall increased at a more rapid rate than usual. The RISA dilution method may be used as the basis for study of molecular transformations within the FPF compartment as reported in the accompanying paper (26 RISA) dilution method to measure lamb fetal pulmonary fluid (FPF) volume, we followed the disappearance of proteincomplexed, ' C-labeled phosphatidylcholine ([' C] PC) during the first 90 min after its injection into FPF. The FPF s a m p l e s w e r e a n a l y z e d f o r t o t a l lipid 14C activity and f o r distribution of 14C in PC, other phospholipids (PL), fatty acids (FA), and neutral lipids (NL).…”

Section: Discussionmentioning

confidence: 99%

“…In the present paper the [' ' I] RISA dilution method is validated, FPF volume determinations are presented, and their significance is discussed. The fate of FPF phosphatidylcholine, determined on the basis of FPF dilution, is described in the accompanying report (26).…”

Section: -mentioning

confidence: 99%

Lamb Fetal Pulmonary Fluid. I. Validation and Significance of Method for Determination of Volume and Volume Change

1975

Self Cite

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ExtractAn indicator-dilution method is described for measuring fetal pulmonary fluid, FPF, volume (Ve), and volume change with time (VS) in the lamb fetus in utero. The indicator, albumin, which is the predominant protein of normal FPF, was administered directly to FPF in the form of radioiodinated human serum albumin (RISA). We have shown that (I) RISA is disbributed homogeneously throughout the FPF compartment, (2) it is not altered within FPF, (3) it does not alter the functional characteristics of FPF, and (4) it remains within the FPF compartment throughout the period of measurement. Ve varies directly with the weight of the fetus so that Ve per kilogram was 31.6-35.6 ml/kg in four of five fetuses. The Ve per kilogram was lower in one fetus at the start of the experiment but increased rapidly to 29.0 ml/kg within 45 min. Thus the relaxation volume of the fetus in utero is in the range of functional residual capacity (FRC) and thoracic gas volume of the air-breathing neonate, which indicates that the pulmonary transformation at birth is, in essence, an isovolumic change in state wherein the fetal liquid-lung becomes the neonatal air-lung. The rate at which FPF is formed (Vs) is about 1.5 ml/hr-kg; however, the rate may change in so far as both rapid increases and rapid decreases of FPF volume were observed. When breathing movements were induced in the fetus by stimulation of the sciatic nerve, FPF volume decreased rapidly (Vs = -3.0 ml/hrkg), which indicates that negative intrathoracic pressures promote its resorption. When FPF volume fell transiently or was low initially, there was subsequent rapid restoration of volume to the range of anticipated FRC.

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Lamb Fetal Pulmonary Fluid. II. Fate of Phosphatidylcholine

Cited by 27 publications

References 2 publications

The Labeling of Pulmonary Surfactant Phosphatidylcholine in Newborn and Adult Sheep

The Labeling of Pulmonary Surfactant Phosphatidylcholine in Newborn and Adult Sheep

Biochemical characterization of pulmonary washings of patients with alveolar proteinosis, interstitial pneumonitis and alveolar cell carcinoma.

Lamb Fetal Pulmonary Fluid. I. Validation and Significance of Method for Determination of Volume and Volume Change

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