LAMB1 Is Related to the T Stage and Indicates Poor Prognosis in Gastric Cancer

Abstract

Background and Objective: Gastric cancer (GC) is a common tumor malignancy with high incidence and poor prognosis. Laminin is an indispensable component of basement membrane and extracellular matrix, which is responsible for bridging the internal and external environment of cells and transmitting signals. This study mainly explored the association of the LAMB1 expression with clinicopathological characteristics and prognosis in gastric cancer. Methods: The expression data and clinical information of gastric cancer patients were downloaded from The Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG). And we analyzed the relationship between LAMB1 expression and clinical characteristics through R. CIBERSORTx was used to calculate the absolute score of immune cells in gastric tumor tissues. Then COX proportional hazard models and Kaplan-Meier curves were performed to evaluate the role of LAMB1 and its influence on prognosis in gastric cancer patients. Finally, GO and KEGG analysis were applied for LAMB1-related genes in gastric cancer, and PPI network was constructed in Cytoscape software. Results: In the TCGA cohort, patients with gastric cancer frequently generated LAMB1 gene copy number variation, but had little effect on mRNA expression. Both in the TCGA and ACRG cohorts, the mRNA expression of LAMB1 in gastric cancer tissues was higher than it in normal tissues. All patients were divided into high expression group and low expression group according to the median expression level of LAMB1. The elevated expression group obviously had more advanced cases and higher infiltration levels of M2 macrophages. COX proportional hazard models and Kaplan-Meier curves revealed that patients with enhanced expression of LAMB1 have a worse prognosis. GO/KEGG analysis showed that LAMB1-related genes were enriched in PI3K-Akt signaling pathway, focal adhesion, ECM-receptor interaction, etc. Conclusions: The high expression of LAMB1 in gastric cancer is related to the poor prognosis of patients, and it may be related to microenvironmental changes in tumors.

Exploring the molecular mechanism of hepatitis virus inducing hepatocellular carcinoma by microarray data and immune infiltrates analysis

“…Since a large number of M2-type macrophages infiltrate HCC caused by HBV and HCV, and the activation of PI3K-Akt-mTOR pathway is related to the M2 polarization of macrophages (11,24), Through literature review, it was found that 13 differential genes (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36), including mTOR, PIK3CA, AKT2, and PIK3CB, involved in the M2-type polarization of macrophages, according to the genes related to M2-type polarization. We first analyzed the expression of these 13 genes in HBV-HCC and HCV-HCC, and found that mTOR was down-regulated while other genes were up-regulated (Figure S3), which was consistent with the molecular characteristics of macrophage polarization.…”

HIF1A overexpression predicts the high lymph node metastasis risk and indicates a poor prognosis in papillary thyroid cancer

“…Dendritic cells were increased in thyroid cancer tissue, suppressing the immune response [ 41 ]. Macrophages M2 favor the Th2 immune response, promoting tumor progression [ 42 ]. Taken together, HIF1A expression exhibited negative correlations with anti-tumor immunity and presented a positive association with tumor-suppressive immunity.…”

Comprehensive analysis of tissue proteomics in patients with papillary thyroid microcarcinoma uncovers the underlying mechanism of lymph node metastasis and its significant sex disparities

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