Laminaria digitata as a potential carbon source for succinic acid and bioenergy production in a biorefinery perspective

Bio‐based succinic acid: an overview of strain development, substrate utilization, and downstream purification

“…Softwood dilute acid hydrolysates could also be used for succinic acid production using the modified strain E. coli AFP184 and 42.2 g L −1 of succinic acid could be accumulated with a yield of 0.72 g g −1 . Algal hydrolysates contain a high concentration of carbohydrate, such as 45.0 g L −1 of glucose and 7.5 g L −1 of mannitol and A. succinogenes 130Z has the ability to convert algal hydrolysates to 33.8 g L −1 of succinic acid with productivity of 1.50 g L −1 h −1 . Plant residues, which are another lignocellulose‐rich material, were limited in their use for succinic acid production due to the complex and expensive pretreatment processes.…”

Demonstration of parallel algal processing: production of renewable diesel blendstock and a high-value chemical intermediate

“…Previous A. succinogenes 130Z batch fermentations of macroalgae achieved productivities of 0.3-3.9 g L −1 h −1 and yields of 0.73-0.92 g g −1 total sugars. [13][14][15] It must be noted that our titer values are a function of the sugar concentration in the PAL and so comparisons with other sugar sources are not necessarily applicable. Our yields and productivity values are lower than those seen with other sugar sources and we attribute that to the higher salt concentration introduced during the pretreatment and neutralization steps.…”

“…Using A. succinogenes 130Z, batch fermentations of sugars released using enzymatic hydrolysis from Saccharina latissimi or Laminaria digitata achieved maximum titers, yields, and productivities of 36.8 g L −1 , 0.92 g g −1 sugars, and 3.9 g L −1 h −1 and 33.8 g L −1 , 0.87 g g −1 sugars, and 0.5 g L −1 h −1 respectively. 13,14 Similarly, sugars released from Palmaria palmata using a two-step hydrochloric acid followed by enzymatic hydrolysis pretreatment were fermented to produce succinic acid by an engineered E. coli strain that achieved a maximum titer, yield, and productivity of 22.4 g L −1 , 0.73 g g −1 total sugars, and 0.3 g L −1 h −1 respectively. 15 Recently, combined algal processing (CAP) demonstrated integrated biofuel production from pretreated algal biomass.…”

Saccharification of Brown Macroalgae Using an Arsenal of Recombinant Alginate Lyases: Potential Application in the Biorefinery Process

“…In the past years, uronic acids from brown algae produced by endo and exo alginate lyases have become an attractive and sustainable source of fermentable sugars which could be turned into bioethanol, biogas, or some other commodities provided the appropriate metabolic pathways are present [22][23][24][25][26][27][28][29][30]. On the other hand, the degradation of alginate has been shown to be favorable for the extraction of mannitol and cellulose from algal biomass for using them as alternative sugar sources [31][32][33][34][35][36]. Alginate lyases have also been proposed for "enzyme-assisted extractions" of highvalue bioactives from brown macroalgae such as carotenoids, fluorescent pigments, fucoidan, and phlorotannins [37][38][39][40].…”