Laminarin from Seaweed (Laminaria japonica) Inhibits Hepatocellular Carcinoma Through Upregulating Senescence Marker Protein-30

Abstract

Objective: This study aimed at investigating the specific roles of laminarin from seaweed (Laminaria japonica) in hepatocellular carcinoma (HCC) and its potential mechanisms related to senescence marker protein-30 (SMP-30). Materials and Methods: Human HCC cell lines, including Bel-7404 and HepG2, were incubated with different concentrations of laminarin (0, 5, 15, 25, 35, and 45 mg/mL). The cell viability and apoptosis rates were detected by WST-8 cell proliferation assay and flow cytometry, respectively. Hepa 1-6 tumor-bearing mice were injected with different concentrations of laminarin (400, 800, and 1200 mg/kg$d), and tumor volume and weight were measured. The expression of SMP-30 was detected in laminarin-treated Bel-7404 and HepG2 HCC cells and LO2 normal liver cells by quantitative real-time PCR and Western blotting. Results: The treatment with laminarin (48 h) significantly decreased the viability and increased the apoptosis rates of Bel-7404 and HepG2 cells in a dose-dependent manner. The injection of laminarin also significantly decreased the tumor volumes (beginning on the 10th day) and tumor weights (30 d post-injection) of mice in a dose-dependent manner. In addition, the treatment with laminarin (35 mg/mL for 48 h) significantly upregulated SMP-30 in Bel-7404 and HepG2 cells but not in LO2 cells. Conclusion: Laminarin inhibited the proliferation of Bel-7404 and HepG2 cells and inhibited the growth of tumors in Hepa 1-6 tumor-bearing mice by upregulating SMP-30.

Characteristics of Marine Biomaterials and Their Applications in Biomedicine

“…Laminarin possesses a wide range of biological activities, including antitumor, antioxidant, anti-inflammatory, and other biofunctional activities [70,74]. Lin Tian et al evaluated the antitumor roles of laminarin from seaweed (Laminaria japonica) [127]. The results showed that laminarin significantly inhibited the proliferation and promoted the apoptosis of BEL-7404 and HepG2 cells in vitro, and it also significantly inhibited the growth of the tumor in Hepa 1-6 tumor-bearing mice [127].…”

“…Lin Tian et al evaluated the antitumor roles of laminarin from seaweed (Laminaria japonica) [127]. The results showed that laminarin significantly inhibited the proliferation and promoted the apoptosis of BEL-7404 and HepG2 cells in vitro, and it also significantly inhibited the growth of the tumor in Hepa 1-6 tumor-bearing mice [127]. The regulatory mechanism of Laminaria in tumors is to increase the expression of senescence marker protein-30 (SMP-30) in BEL-7404 and HepG2 cells.…”

Seaweed and Iodine Intakes and SLC5A5 rs77277498 in Relation to Thyroid Cancer

“…(dashima) has been proven to exert cytotoxic activity against various cancer types including breast, colon, hepatocellular, lung, and prostate cancer cells, by inhibiting proliferation and inducing apoptosis via both mitochondrialdependent and death receptor rough extrinsic-related pathways [25][26][27][28][29][30]. In addition, similar anticancer effects have been reported for bioactive compounds extracted from Laminaria japonica, such as glycoproteins, polysaccharides, laminarin, fucoxanthin, and phlorotannin [31][32][33][34][35].…”

Seaweed Exhibits Therapeutic Properties against Chronic Diseases: An Overview

“…Moreover, Hizikia fusiforme inhibited EJ tumor growth both in vivo and in vitro through G1-phase cell cycle arrest by downregulating cyclins and cyclin-dependent kinases (CDKs), as well as by inhibiting the expression of MMP-9 by downregulating NF-κB, AP-1, and Sp-1 [122]. In addition, seaweed extract also exhibited potent anticancer activity against hepatocellular carcinoma (HCC) cell lines [123], the U87MG cell line [129], and sarcoma 180 ascites tumor cells both in vitro and in vivo [126]. Furthermore, the inhibition of HT-29 cell proliferation by fucoidan may be due to the downregulation of IGF-IR signaling by the main IRS-1/PI3K/AKT pathway.…”