Laminarin Promotes Immune Responses and Normalizes Glutamic Oxaloacetic Transaminase and Glutamic Pyruvic Transaminase Levels in Leukemic Mice In Vivo

Abstract

Laminarin did not affect animal appearances, but increased the body weight at all doses. It reduced the weight of liver at 2.5 and 5 mg/ml and of spleen at 5 mg/ml. Laminarin increased CD3 (2.5 mg/ml) and CD19 (1 and 5 mg/ml) cell populations but reduced CD11b (5 mg/ml) cell populations, however, these did not affect Mac-3 marker level. Laminarin at 1 mg/ml increased phagocytosis by macrophages from peripheral blood mononuclear cell, but did not affect those from the peritoneal cavity. Laminarin increased NK cell cytotoxic activity at all doses and at a target ratio of 25:1 and 50:1. Laminarin did not affect B-cell proliferation, but at 5 mg/ml significantly reduced T-cell proliferation. Laminarin restored glutamate oxaloacetate transaminase (2.5 and 5 mg/ml) and glutamate pyruvate transaminase (2.5 mg/ml) levels. Based on these results, we suggest that laminarin can promote immune responses and protect against liver injury.

Effects of Laminaria Japonica Polysaccharides on the Survival of Non-Small-Cell Lung Cancer A549 Cells

“…Recent years have witnessed increasing attention of many plants due to their wide range of biological activities. Polysaccharides from Laminaria japonica have also been shown to possess a variety of biological functions, including antioxidation, antivirus, and tumor growth inhibition [4][5][6][7][8][9][10][11][12][13][14]. Therefore, we studied the human non-small-cell lung cancer A549 cells and discussed the effects of Laminaria japonica polysaccharides on its growth in vivo and vitro and its possible mechanism.…”

“…Studies have found that Laminaria japonica polysaccharides have a variety of biological activities, including promoting fertility [4], alleviating metabolic syndrome [5], preventing vascular calcification [6], resisting virus [7,8], immunoregulating [9], and antioxidating [10]. In addition, Laminaria japonica polysaccharides have inhibitory effect on a variety of tumor cells, such as liver cancer cells, nasopharyngeal carcinoma cells, cervical cancer cells, and leukemia cells [11][12][13][14], but there are no reports on the effect of Laminaria japonica polysaccharides on human non-small-cell lung cancer A549 cells. In this study, we investigated the effects of Laminaria japonica polysaccharides on the survival of A549 cells in vitro and in vivo and its possible mechanism.…”

Maslinic Acid Enhances Immune Responses in Leukemic Mice Through Macrophage Phagocytosis and Natural Killer Cell Activities In Vivo

“…Murine myelomonocytic leukemia cells (WEHI-3 cells) and YAC-1 were obtained from the Food Industry Research and Development Institute (Hsinchu, Taiwan, R.O.C.). About 1×10 6 cells/ml in IMDM or RPMI-1640 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin, 100 μg/ml streptomycin and 2 mM L-glutamine were placed in 75 cm 2 tissue culture flasks under humidified 5% CO 2 at 37˚C as previously described (25).…”

“…The animal study project was reviewed and approved by the Institutional Animal Care and Use Committee of China Medical University (approval ID: 2017-009-1). In vivo experiments were carried out in accordance with the institutional animal ethical guidelines of the China Medical University as described previously (25).…”

“…Treatment of animals with MA. The in vivo immune responses of MA were performed by analysis of leukocyte populations and associated activities as previously described (25). In brief, 48 mice were randomly divided into six groups (n=8).…”