Laminated collagen-fiber bio-composites for soft-tissue bio-mimetics

Sharabi, Mirit; Benayahu, Dafna; Benayahu, Yehuda; Isaacs, Jessica L.; Haj-Ali, Rami

doi:10.1016/j.compscitech.2015.06.024

Cited by 34 publications

(43 citation statements)

References 35 publications

(39 reference statements)

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“…The cells' functionality performance indicates and suggests that the scaffold is suitable to be developed as a new medical device that has the potential to support regeneration and the production of functional tissue.Biomolecules 2020, 10, 458 2 of 11 new tissue to improves regeneration and healing processes [1,[5][6][7][8][9][10][11]. The use of naturally made scaffolds is the best approach, but they may lack the physical/mechanical properties needed for the new tissue formed.We have developed a composite biomaterial scaffold made from collagen fibers embedded in alginate hydrogel [12][13][14][15][16][17]. The collagen used as a biopolymer for producing implantable medical devices is usually obtained through chemical extraction and reconstitution processes, resulting in products with poor mechanical strength [18][19][20].…”

mentioning

confidence: 99%

“…The collagen used as a biopolymer for producing implantable medical devices is usually obtained through chemical extraction and reconstitution processes, resulting in products with poor mechanical strength [18][19][20]. However, the biocomposite we describe here focuses on the unique coral-derived collagen fibers that were recently identified for their molecular composition and structure [13,14] and mechanical properties [12,[15][16][17]. These attributes make our biocomposite uniquely suitable for use in a new generation of scaffolds that can be tailored to meet the mechanical properties of various target tissues.…”

mentioning

confidence: 99%

“…We have developed a composite biomaterial scaffold made from collagen fibers embedded in alginate hydrogel [12][13][14][15][16][17]. The collagen used as a biopolymer for producing implantable medical devices is usually obtained through chemical extraction and reconstitution processes, resulting in products with poor mechanical strength [18][19][20].…”

mentioning

confidence: 99%

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Mesenchymal Cell Growth and Differentiation on a New Biocomposite Material: A Promising Model for Regeneration Therapy

Pomeraniec

Benayahu

2020

Biomolecules

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Mesenchymal stem cells serve as the body's reservoir for healing and tissue regeneration. In cases of severe tissue trauma where there is also a need for tissue organization, a scaffold may be of use to support the cells in the damaged tissue. Such a scaffold should be composed of a material that can biomimic the mechanical and biological properties of the target tissues in order to support autologous cell-adhesion, their proliferation, and differentiation. In this study, we developed and assayed a new biocomposite made of unique collagen fibers and alginate hydrogel that was assessed for the ability to support mesenchymal cell-proliferation and differentiation. Analysis over 11 weeks in vitro demonstrated that the scaffold was biocompatible and supports the cells viability and differentiation to produce tissue-like structures or become adipocyte under differentiation medium. When the biocomposite was enriched with nano particles (NPs), mesenchymal cells grew well after uptake of fluorescein isothiocyanate (FITC) labeled NPs, maintained their viability, migrated through the biocomposite, reached, and adhered to the tissue culture dish. These promising findings revealed that the scaffold supports the growth and differentiation of mesenchymal cells that demonstrate their full physiological function with no sign of material toxicity. The cells' functionality performance indicates and suggests that the scaffold is suitable to be developed as a new medical device that has the potential to support regeneration and the production of functional tissue.Biomolecules 2020, 10, 458 2 of 11 new tissue to improves regeneration and healing processes [1,[5][6][7][8][9][10][11]. The use of naturally made scaffolds is the best approach, but they may lack the physical/mechanical properties needed for the new tissue formed.We have developed a composite biomaterial scaffold made from collagen fibers embedded in alginate hydrogel [12][13][14][15][16][17]. The collagen used as a biopolymer for producing implantable medical devices is usually obtained through chemical extraction and reconstitution processes, resulting in products with poor mechanical strength [18][19][20]. However, the biocomposite we describe here focuses on the unique coral-derived collagen fibers that were recently identified for their molecular composition and structure [13,14] and mechanical properties [12,[15][16][17]. These attributes make our biocomposite uniquely suitable for use in a new generation of scaffolds that can be tailored to meet the mechanical properties of various target tissues. The biological and mechanical properties of these collagen fibers facilitate the desired physiological activity, together with the promotion of cell proliferation and differentiation. The collagen fibers are embedded in an alginate hydrogel, a polysaccharide extracted from algae that has already been applied in a wide spectrum of uses such as food, pharmaceutics, and medical device industries [21][22][23][24]. The addition of the collagen fibers reinforces the alginate in ...

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confidence: 99%

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confidence: 99%

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Mesenchymal Cell Growth and Differentiation on a New Biocomposite Material: A Promising Model for Regeneration Therapy

Pomeraniec

Benayahu

2020

Biomolecules

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“…In order to improve performance of such natural polymers and expand the applied range, they has been blended with various of other polymers which include both natural macromolecules like collagen, gelatin, elastin, chitosan, hyaluronic and sodium carboxymethyl cellulose as well as synthetic molecules like polyacrylamide, polystyrene, polyurethane, poly (L-lactide), polyvinylalcohol, poly ethylene glycol11202122232425262728293031. Those blend scaffolds with more superior properties were employed for various tissue engineering applications.…”

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confidence: 99%

A Biomimetic Silk Fibroin/Sodium Alginate Composite Scaffold for Soft Tissue Engineering

Wang

Shi

et al. 2016

Sci Rep

156

103

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A cytocompatible porous scaffold mimicking the properties of extracellular matrices (ECMs) has great potential in promoting cellular attachment and proliferation for tissue regeneration. A biomimetic scaffold was prepared using silk fibroin (SF)/sodium alginate (SA) in which regular and uniform pore morphology can be formed through a facile freeze-dried method. The scanning electron microscopy (SEM) studies showed the presence of interconnected pores, mostly spread over the entire scaffold with pore diameter around 54~532 μm and porosity 66~94%. With significantly better water stability and high swelling ratios, the blend scaffolds crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) provided sufficient time for the formation of neo-tissue and ECMs during tissue regeneration. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) results confirmed random coil structure and silk I conformation were maintained in the blend scaffolds. What’s more, FI-TR spectra demonstrated crosslinking reactions occurred actually among EDC, SF and SA macromolecules, which kept integrity of the scaffolds under physiological environment. The suitable pore structure and improved equilibrium swelling capacity of this scaffold could imitate biochemical cues of natural skin ECMs for guiding spatial organization and proliferation of cells in vitro, indicating its potential candidate material for soft tissue engineering.

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“…Moreover, cellulose fibre is easy in handling and process for experimental and installation purpose compare to animal and mineral fibre. Utilization of natural fibre composites (NFCs) was introduced in many applications such as automotive, construction, food packaging and medication [1][2][3][4]. Natural fibre composites are a combination of two materials which are natural fibre itself and the matrix.…”

Section: Introductionmentioning

confidence: 99%

Multi-criteria decision-making tools for material selection of natural fibre composites: A review

Muhammad¹,

Sapuan²,

Mastura³

2018

J. MECH. ENG. SCI.

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Materials selection in manufacturing process is an important stage and should be performed in parallel with selection of manufacturing process. In automotive industry, production of green automotive component could utilize the natural sources such as plant fibres. In recent years, several multi-criteria decision-making (MCDM) techniques are suggested to choose the best materials for particular application. Materials selection tools for natural fibre composites are studied from past researchers with the summary of the advantage and disadvantages. In addition, new optimization approach in materials selection by using statistical analysis such as multiple linear regression (MLR), response surface methodology (RSM) and Taguchi method (TM) is proposed in this study. The proposed method could evaluate the criteria or attribute in materials selection precisely by analyse the relationship of the parameters, goodness of fit, correlation, analysis of variance (ANOVA), determination of coefficient and the significant criteria in to the desired goal of the design problem.

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Laminated collagen-fiber bio-composites for soft-tissue bio-mimetics

Cited by 34 publications

References 35 publications

Mesenchymal Cell Growth and Differentiation on a New Biocomposite Material: A Promising Model for Regeneration Therapy

Mesenchymal Cell Growth and Differentiation on a New Biocomposite Material: A Promising Model for Regeneration Therapy

A Biomimetic Silk Fibroin/Sodium Alginate Composite Scaffold for Soft Tissue Engineering

Multi-criteria decision-making tools for material selection of natural fibre composites: A review

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