Lamiophlomis herba: A comprehensive overview of its chemical constituents, pharmacology, clinical applications, and quality control

Yu, Li; Fang, Li; Zheng, Tingting; Shi, Lei; Zhang, Zhigang; Niu, Tianmei; Wang, Qiuyue; Dong-sheng, Zhao; Li, Wei; Zhao, Peng

doi:10.1016/j.biopha.2021.112299

Cited by 18 publications

(43 citation statements)

References 35 publications

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“…Natural products (NPs) often serve as critical precursors in the field of drug development. Tibetan medicine is one of the most comprehensive traditional medical systems in the world [5][6][7]. The search for potential anti-inflammatory active substances derived from traditional Tibetan medicines has thus emerged as a promising approach to the development of novel drugs to prevent or treat inflammatory diseases.…”

Section: Introductionmentioning

confidence: 99%

Screening and Isolation of Potential Anti-Inflammatory Compounds from Saxifraga atrata via Affinity Ultrafiltration-HPLC and Multi-Target Molecular Docking Analyses

Fang

et al. 2022

Nutrients

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In this study, a 100 g sample of Saxifraga atrata was processed to separate 1.3 g of 11-O-(4′-O-methylgalloyl)-bergenin (Fr1) after 1 cycle of MCI GEL® CHP20P medium pressure liquid chromatography using methanol/water. Subsequently, COX-2 affinity ultrafiltration coupled with reversed-phase liquid chromatography was successfully used to screen for potential COX-2 ligands in this target fraction (Fr1). After 20 reversed-phase liquid chromatography runs, 74.1 mg of >99% pure 11-O-(4′-O-methylgalloyl)-bergenin (Fr11) was obtained. In addition, the anti-inflammatory activity of 11-O-(4′-O-methylgalloyl)-bergenin was further validated through molecular docking analyses which suggested it was capable of binding strongly to ALOX15, iNOS, ERBB2, SELE, and NF-κB. As such, the AA metabolism, MAPK, and NF-κB signaling pathways were hypothesized to be the main pathways through which 11-O-(4′-O-methylgalloyl)-bergenin regulates inflammatory responses, potentially functioning by reducing pro-inflammatory cytokine production, blocking pro-inflammatory factor binding to cognate receptors and inhibiting the expression of key proteins. In summary, affinity ultrafiltration-HPLC coupling technology can rapidly screen for multi-target bioactive components and when combined with molecular docking analyses, this approach can further elucidate the pharmacological mechanisms of action for these compounds, providing valuable information to guide the further development of new multi-target drugs derived from natural products.

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Section: Introductionmentioning

confidence: 99%

Screening and Isolation of Potential Anti-Inflammatory Compounds from Saxifraga atrata via Affinity Ultrafiltration-HPLC and Multi-Target Molecular Docking Analyses

Fang

et al. 2022

Nutrients

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“…Kudo ( L. rotata ) is a perennial wild herb of the Lamiaceae family, which is widely distributed in the Qinghai, Tibet, Sichuan, and Gansu provinces of China. It grows in meadow, grassland, and sand gravel regions at altitudes between 3100 and 5100 m. It is a commonly used Chinese herbal medicine of Tibetan, Naxi, Mongolian, and other ethnic groups in China [ 15 , 16 ]. The roots and leaves of L. rotata contain various active ingredients, such as iridoids, flavonoids, and phenylethanol glycosides [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…The iridoid compounds, represented by gardenoside methyl ester and 8-O-acetyl gardenoside methyl ester, which have good hemostatic and procoagulant effects, are the main medicinal components of L. rotata [ 22 ]. Present research on L. rotata mainly focuses on the distribution of germplasm resources [ 15 ], the extraction, separation, and identification of chemical components [ 19 ], the pharmacological effects [ 23 ], and other aspects. However, research on the biosynthetic pathway of iridoid, flavonoid, and phenylethanol glycoside compounds in L. rotata has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Candidate Reference Genes for Gene Expression Analysis in Wild Lamiophlomis rotata

Wang

Qian

Geng

et al. 2023

Genes

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Lamiophlomis rotata (Benth.) Kudo is a perennial and unique medicinal plant of the Qinghai–Tibet Plateau. It has the effects of diminishing inflammation, activating blood circulation, removing blood stasis, reducing swelling, and relieving pain. However, thus far, reliable reference gene identifications have not been reported in wild L. rotata. In this study, we identified suitable reference genes for the analysis of gene expression related to the medicinal compound synthesis in wild L. rotata subjected to five different-altitude habitats. Based on the RNA-Seq data of wild L. rotata from five different regions, the stability of 15 candidate internal reference genes was analyzed using geNorm, NormFinder, BestKeeper, and RefFinder. TFIIS, EF-1α, and CYP22 were the most suitable internal reference genes in the leaves of L. rotata from different regions, while OBP, TFIIS, and CYP22 were the optimal reference genes in the roots of L. rotata. The reference genes identified here would be very useful for gene expression studies with different tissues in L. rotata from different habitats.

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“…The above-ground parts are used to treat grasserie, fracture, injuries from falls, osteomyelitis, gunshot injury, and edema pain. The function of the underground parts is to increase blood circulation, remove stasis and detumescence, and act as an analgesic [2,[5][6][7]. The whole plant can also be collected for medicinal use [2,[5][6].…”

Section: Introductionmentioning

confidence: 99%

“…The whole plant can also be collected for medicinal use [2,[5][6]. In present, only the aerial part can be used indicated by the Pharmacopoeia of the People's Republic of China (Volume Ⅰ) 2020 edition [4] and the digging for the root is banned because PR is now listed as a first-class 2 for more than 2000 years in the traditional Tibetan medicine known as "Daba" and "Dabuba" [4][5][6][7]. Generally, PR is used directly in the clinic without any prior processing, and commonly for pain relief [6].…”

Section: Introductionmentioning

confidence: 99%

Chemicals in Essential Oils of <em><i>Phlomoides rotata</i></em> (Benth. ex Hook. f.) Mathiesen and Their Antioxidant Activities Potential

Pan,

Xie,

Yan

et al. 2023

Preprint

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Due to the low content, few studies are focused on the essential oils (EOs) of Phlomoides rotata (Benth. ex Hook. f.) Mathiesen (syn. Lamiophlomis rotata (Benth.) Kudô. This plant has been used to treat rheumatic arthritis and grasserie in China. However, such EOs may have important pharmacological activities such as anti-cancer. To identify the chemical markers (CM) in the EOs and evaluate their antioxidant activities (AAs), we firstly conduct a thoroughly investigation on their chemicals and AAs, to the best of our knowledge. Light yellow EOs with fresh and elegant smell are obtained by hydro-distillation with average yield 0.11% (volume mL/weight g). The crystals are separated from the EOs through cryoprecipitation, respectively. A total of 81 components are qualified and quantified in the EOs, crystals and EOs removed crystals, in which 44 ones are first reported. As for content, the main compounds are long-chain fatty acids (FAs) and their esters. The most abundant one n-hexadecanoic acid (palmitic acid, PA) accounting for 43.15-54.8%, 58.49-64.57% and 15.9-41.1%, in the EOs, crystals and EOs removed crystals, respectively. Seven compounds including PA, tetradecanoic acid, linoleic acid, oleic acid, methyl hexadecanoate, hexahydrofarnesyl acetone and phytol can be chosen a the CMs in these EOs. The AAs are evaluated through in vitro assays. PA presents pro-oxidant activities in a concentration dependence manner. Usually, the EOs removed crystals demonstrate stronger AAs, and the crystals demonstrate weaker AAs compared with that of the corresponding EOs, which is related to the different content of PA in these samples. This study can give some hints for the utilization of such EOs which are abundant in FAs such as PA.

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Lamiophlomis herba: A comprehensive overview of its chemical constituents, pharmacology, clinical applications, and quality control

Cited by 18 publications

References 35 publications

Screening and Isolation of Potential Anti-Inflammatory Compounds from Saxifraga atrata via Affinity Ultrafiltration-HPLC and Multi-Target Molecular Docking Analyses

Screening and Isolation of Potential Anti-Inflammatory Compounds from Saxifraga atrata via Affinity Ultrafiltration-HPLC and Multi-Target Molecular Docking Analyses

Evaluation of Candidate Reference Genes for Gene Expression Analysis in Wild Lamiophlomis rotata

Chemicals in Essential Oils of <em><i>Phlomoides rotata</i></em> (Benth. ex Hook. f.) Mathiesen and Their Antioxidant Activities Potential

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