Land use and host neighbor identity effects on arbuscular mycorrhizal fungal community composition in focal plant rhizosphere

Morris, E. Kathryn; Buscot, François; Herbst, Christine; Meiners, Torsten; Obermaier, Elisabeth; Wäschke, Nicole; Wubet, Tesfaye; Rillig, Matthias C.

doi:10.1007/s10531-013-0527-z

Cited by 41 publications

(24 citation statements)

References 34 publications

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“…A semi-nested PCR protocol was used to amplify the %630 bp-long small subunit (SSU) region of the AMF 18S rDNA via pyrosequencing analysis (454 GS-FLX, Roche). In the first PCR run (PCRI) a Glomeromycota-specific region was amplified with the primer set GLOMERWT0/GLOMER1536 (Wubet et al, 2006), followed by the semi-nested second PCR reaction with the forward general fungal primer NS31 (Simon et al, 1992) including the A adaptor and a 10 bp multiplex identifier (1 of 60 different MIDs), and the B adaptor including the reverse modified AMF primer AM1a and AM1b (Morris et al, 2013). The first PCR was carried out at a 25 μl reaction volume with 0.5 μl of diluted DNA template (5-20 ng μl −1 ), 12.5 μl GoTaq Green Mastermix 2× (Promega, Mannheim, Germany), 1 μl of each primer (25 μM) on an Eppendorf Mastercycler DNA Engine Thermal Cycler PCR (Eppendorf, Hamburg, Germany) with the following PCR conditions: 98 C for 30 s, 5 cycles of 94 C for 30 s, 60 C (−1 C/cycle, 4 cycles) for 30 s, 72 C for 1 min and 25 cycles of 94 C for 30 s, 55 C for 30 s, 72 C for 1 min, and for extension 72 C for 5 min.…”

Section: Dna Extraction and Pyrosequencing Of Amf Ampliconsmentioning

confidence: 99%

Unraveling spatiotemporal variability of arbuscular mycorrhizal fungi in a temperate grassland plot

Goldmann

Boeddinghaus

Klemmer

et al. 2019

Environmental Microbiology

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Summary Soils provide a heterogeneous environment varying in space and time; consequently, the biodiversity of soil microorganisms also differs spatially and temporally. For soil microbes tightly associated with plant roots, such as arbuscular mycorrhizal fungi (AMF), the diversity of plant partners and seasonal variability in trophic exchanges between the symbionts introduce additional heterogeneity. To clarify the impact of such heterogeneity, we investigated spatiotemporal variation in AMF diversity on a plot scale (10 × 10 m) in a grassland managed at low intensity in southwest Germany. AMF diversity was determined using 18S rDNA pyrosequencing analysis of 360 soil samples taken at six time points within a year. We observed high AMF alpha‐ and beta‐diversity across the plot and at all investigated time points. Relationships were detected between spatiotemporal variation in AMF OTU richness and plant species richness, root biomass, minimal changes in soil texture and pH. The plot was characterized by high AMF turnover rates with a positive spatiotemporal relationship for AMF beta‐diversity. However, environmental variables explained only ≈20% of the variation in AMF communities. This indicates that the observed spatiotemporal richness and community variability of AMF was largely independent of the abiotic environment, but related to plant properties and the cooccurring microbiome.

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Section: Dna Extraction and Pyrosequencing Of Amf Ampliconsmentioning

confidence: 99%

Unraveling spatiotemporal variability of arbuscular mycorrhizal fungi in a temperate grassland plot

Goldmann

Boeddinghaus

Klemmer

et al. 2019

Environmental Microbiology

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“…In a study on Arbuscular Mycorrhizal Fungi (AMF), effects of land use, host plant neighbourhood and spatial arrangement on the AMF composition was tested over 67 grassland plots spread across the three German Biodiversity Exploratories (Morris et al 2013). The authors show that the diversity of AMF react similar sensitive at both, large-and small scales; for example, the ability of AMF to provide protection from pathogens declined under high land-use intensity (Morris et al 2013). …”

Section: Effects Of Abiotic and Biotic Factors On Species Diversitymentioning

confidence: 99%

European grassland ecosystems: threatened hotspots of biodiversity

et al. 2013

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Biodiversity is not homogenously distributed over the globe, and ecosystems differ strongly in the number of species they provide. With this special issue we highlight the ecology and endangerment of one of the most diverse ecosystem of Europe: the European grassland ecosystems. The selected 16 contributions describe interactions from below-ground to the atmosphere and focus on (1) effects of abiotic and biotic on species diversity, (2) the impact of various factors along spatial and temporal gradients, (3) the

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“…we would expect a similar pattern for phyllosphere bacterial communities, becoming more dissimilar with 40 increasing geographic distances between sites. Furthermore, it has to be considered that anthropogenic 41 influences on intensities of land use management types like fertilization, grazing and mowing can have 42 strong effects on soil properties and on plant pheno-and chemotypic characteristics, and therefore may 43 also shape flower and leaf microbiomes (Estendorfer et al, 2017; Li et al, 2018; E. K. Morris et al, 2013; were constructed and differences in relative abundance between groups were tested with t-tests. To 140 identify chosen individual ASVs at the species level, 16S rDNA sequences were compared with available 141 rDNA sequences in GenBank, using the NCBI BLASTN program (Zhang et al, 2000).…”

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confidence: 99%

Changes amid constancy: flower and leaf microbiomes along land use gradients and between bioregions

Gaube

Junker

Keller

2020

Preprint

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Microbial communities inhabiting above-ground parts of plants affect their host's development, fitness and function. Although studies on plant-associated microbes are of growing interest, environmental drivers of flower microbiomes in particular are poorly characterized. In this study, we investigated flower and leaf epiphytic bacterial microbiomes of Ranunculus acris and Trifolium pratense using metabarcoding of 16S ribosomal DNA in three German bioregions and along land-use intensity gradients. Our data suggests that the structures of bacterial communities clearly differed between plant species and tissue types. Also, floral bacterial communities of R. acris showed higher variability in comparison to T. pratense.Bacteria usually associated with pollinators were found solely in flower samples, while such usually associated with the rhizosphere were only present in high abundances on leaves. We identified Pseudomonadaceae, Enterobacteriaceae and Sphingomonadaceae as the most abundant taxa on flowers, while Sphingomonadaceae, Methylobacteriaceae and Cytophagaceae dominated bacterial communities on leaves. We found strong bacterial turnover already for short geographic distances, which however did not increase with the long distances between bioregions. High land use intensity caused phylogenetically less diverse and more homogenous bacterial communities. This was associated with a loss of rare bacterial families. Intensification of mowing and fertilization affected almost all plant associated bacterial communities, while grazing had only minor effects on bacterial structures of T. pratense flowers. However, dominant taxa were mostly resilient to mowing, grazing and fertilization. Despite that, we identified indicator taxa for regularly disturbed environments in flower microbiomes.

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Land use and host neighbor identity effects on arbuscular mycorrhizal fungal community composition in focal plant rhizosphere

Cited by 41 publications

References 34 publications

Unraveling spatiotemporal variability of arbuscular mycorrhizal fungi in a temperate grassland plot

Unraveling spatiotemporal variability of arbuscular mycorrhizal fungi in a temperate grassland plot

European grassland ecosystems: threatened hotspots of biodiversity

Changes amid constancy: flower and leaf microbiomes along land use gradients and between bioregions

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