Cultural ecosystem services (CES) have specific richness and diversity provision patterns related to particular landscape features and land cover forms. Studies of their spatial distribution, however, are quite rare in the Slovak Republic and surrounding countries. This paper links land cover information based on an ecosystem services (ES) matrix, field survey data and GIS method to assess CES supply in two selected Slovak regions. Our main focus is on the ecologically more valuable ‘hot-spots’ where socio-cultural values accumulate. We determined their spatial distribution, and our comparison with lower cultural value areas confirmed that mountainous landscapes have the highest capacity to provide CES. This especially applies to the landscapes under National Park protection. While Slovak forests, rocks and water areas also form essential ecosystems for overall CES provision, the lowest overall capacity is in areas with residential buildings, construction, industrial and other artificial habitats. Finally, a comparison of our results with the National Ecosystem Assessment indicates that our detailed CES assessment will be more effective in supporting future participatory planning and management processes.