Landscaping Crossover Interference Across a Genome

Sun, Lidan; Wang, Jing; Sang, Mengmeng; Jiang, Libo; Zhao, Bingyu; Cheng, Tangran; Zhang, Qixiang; Wu, Rongling

doi:10.1016/j.tplants.2017.06.008

Cited by 9 publications

(9 citation statements)

References 85 publications

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“…Denote the coefficients of coincidence (a measure of crossover interference) between double marker intervals A-B and B-C, double marker intervals B-C and C-D, double marker intervals A-B and C-D, and triple marker intervals A-B, B-C, and C-D by C 1 , C 2 , C 3 , and C 4 , respectively (Sun et al, 2017). Wang J. et al (2016) formulated the relationship between different recombination fractions based on the CoC and derived a process to estimate and test each coefficient, as follows:…”

Section: Methodsmentioning

confidence: 99%

The Genomic Landscape of Crossover Interference in the Desert Tree Populus euphratica

Wang

Jiang

et al. 2019

Front. Genet.

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Crossover (CO) interference is a universal phenomenon by which the occurrence of one CO event inhibits the simultaneous occurrence of other COs along a chromosome. Because of its critical role in the evolution of genome structure and organization, the cytological and molecular mechanisms underlying CO interference have been extensively investigated. However, the genome-wide distribution of CO interference and its interplay with sex-, stress-, and age-induced differentiation remain poorly understood. Multi-point linkage analysis has proven to be a powerful tool for landscaping CO interference, especially within species for which CO mutants are rarely available. We implemented four-point linkage analysis to landscape a detailed picture of how CO interference is distributed through the entire genome of Populus euphratica , the only forest tree that can survive and grow in saline desert. We identified an extensive occurrence of CO interference, and found that its strength depends on the length of chromosomes and the genomic locations within the chromosome. We detected high-order CO interference, possibly suggesting a highly complex mechanism crucial for P. euphratica to grow, reproduce, and evolve in its harsh environment.

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Section: Methodsmentioning

confidence: 99%

The Genomic Landscape of Crossover Interference in the Desert Tree Populus euphratica

Wang

Jiang

et al. 2019

Front. Genet.

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“…Specifically, crossover frequencies at subtelomeric regions are very high in males and very low in females (Giraut et al 2011). In human females, the number of MLH1 foci is greater than in males, while the average crossover frequency is similar between the sexes (Wang et al 2017). This suggests that crossover maturation after designation may be less efficient in females than in males.…”

Section: Sexual Dimorphism In Crossover Regulation During C Elegans mentioning

confidence: 98%

“…Synaptonemal complex-dependent crossover interference is observed in C. elegans (Libuda et al 2013) and in at least one yeast strain (Sym and Roeder 1994; Chen et al 2008). The biological function of crossover interference is largely unknown but it may confer a selective advantage due to co-segregation of functionally related linked genes (Wang et al 2015; Sun et al 2017).…”

Section: Multiple Layers Of Crossover Regulationmentioning

confidence: 99%

Regulation of Crossover Frequency and Distribution during Meiotic Recombination

Saito

Colaiácovo

2017

Cold Spring Harb Symp Quant Biol

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Crossover recombination is essential for generating genetic diversity and promoting accurate chromosome segregation during meiosis. The process of crossover recombination is tightly regulated and is initiated by the formation of programmed meiotic DNA double-strand breaks (DSBs). The number of DSBs is around 10-fold higher than the number of crossovers in most species, because only a limited number of DSBs are repaired as crossovers during meiosis. Moreover, crossovers are not randomly distributed. Most crossovers are located on chromosomal arm regions and both centromeres and telomeres are usually devoid of crossovers. Either loss or mislocalization of crossovers frequently results in chromosome nondisjunction and subsequent aneuploidy, leading to infertility, miscarriages, and birth defects such as Down syndrome. Here, we will review aspects of crossover regulation observed in most species and then focus on crossover regulation in the nematode Caenorhabditis elegans in which both the frequency and distribution of crossovers are tightly controlled. In this system, only a single crossover is formed, usually at an off-centered position, between each pair of homologous chromosomes. We have identified C. elegans mutants with deregulated crossover distribution, and we are analyzing crossover control by using an inducible single DSB system with which a single crossover can be produced at specific genomic positions. These combined studies are revealing novel insights into how crossover position is linked to accurate chromosome segregation.Meiosis is a specialized cell division process that generates haploid gametes from diploid parental germ cells. This reduction in the number of chromosomes is achieved by following a single round of DNA replication with two consecutive cell divisions (meiosis I and II). Homologous chromosomes are separated at meiosis I, and sister chromatids are separated at meiosis II. There are unique chromosomal events that need to take place during prophase to ensure that homologs segregate properly at meiosis I (Fig. 1). Homologous chromosomes need to find each other and pair; these pairing interactions need to be stabilized via the formation of a scaffold known as the synaptonemal complex, which assembles at the interface between paired homologs; and interhomolog recombination needs to take place in order to produce crossovers. Crossover formation is one of the sources of genetic diversity in the population. Moreover, crossovers result in physical attachments (chiasmata) between homologs that, underpinned by cohesion, confer the tension required to properly align the attached homologs (bivalents) at the metaphase plate and then orient them toward opposite poles of the meiosis I spindle.Errors in crossover formation result in chromosome nondisjunction leading to aneuploidy, which causes infertility, miscarriages, birth defects, and cancers.Given the impact of crossover formation on human health and reproductive biology, it is therefore not surprising that crossovers are tightly regulated. For e...

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“…Interference is detectable over the scale of megabases in Arabidopsis (Lynn et al, 2007; Mercier et al, 2015). Although interference has a great impact on chromosomal distribution of crossovers, it will not be discussed in this review as there are numerous articles focusing specifically on this phenomenon (Wang et al, 2015; Sun et al, 2017).…”

Section: General Information About Crossover In Plantsmentioning

confidence: 99%

Where to Cross Over? Defining Crossover Sites in Plants

2018

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It is believed that recombination in meiosis serves to reshuffle genetic material from both parents to increase genetic variation in the progeny. At the same time, the number of crossovers is usually kept at a very low level. As a consequence, many organisms need to make the best possible use from the one or two crossovers that occur per chromosome in meiosis. From this perspective, the decision of where to allocate rare crossover events becomes an important issue, especially in self-pollinating plant species, which experience limited variation due to inbreeding. However, the freedom in crossover allocation is significantly limited by other, genetic and non-genetic factors, including chromatin structure. Here we summarize recent progress in our understanding of those processes with a special emphasis on plant genomes. First, we focus on factors which influence the distribution of recombination initiation sites and discuss their effects at both, the single hotspot level and at the chromosome scale. We also briefly explain the aspects of hotspot evolution and their regulation. Next, we analyze how recombination initiation sites translate into the development of crossovers and their location. Moreover, we provide an overview of the sequence polymorphism impact on crossover formation and chromosomal distribution.

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Landscaping Crossover Interference Across a Genome

Cited by 9 publications

References 85 publications

The Genomic Landscape of Crossover Interference in the Desert Tree Populus euphratica

The Genomic Landscape of Crossover Interference in the Desert Tree Populus euphratica

Regulation of Crossover Frequency and Distribution during Meiotic Recombination

Where to Cross Over? Defining Crossover Sites in Plants

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