Langmuir–Blodgett film based biosensor for estimation of phenol derivatives

Cabaj, Joanna; Sołoducho, Jadwiga; Nowakowska-Oleksy, Anna

doi:10.1016/j.snb.2009.09.047

Cited by 48 publications

(54 citation statements)

References 31 publications

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“…The response of the biosensors was registered in the range from −0.5 V to +0.5 V at a scan rate of 0.050 V × s −1 . In previous works, it has been demonstrated that the presence of lutetium bisphthalocyanine (LuPc 2 ) electron mediator improves the sensitivity of the Langmuir-Blodgett biosensors towards phenols [31][32][33]. A similar effect has been observed here for CPE biosensors.…”

Section: Electrochemical Response Towards Phenolssupporting

confidence: 82%

“…Numerous amperometric biosensors for the determination of phenolic compounds have been developed using tyrosinase immobilized on different electrode supports. The immobilization methods include carbon paste biosensors [27], conducting polymer modified electrodes [28,29], biosensors based on silica sol-gel composite films [30], Langmuir-Blodgett (LB) thin films [31][32][33], Layer-by-layer (LbL) films [34], and self-assembled monolayers [35].…”

Section: Introductionmentioning

confidence: 99%

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Advantages of the Biomimetic Nanostructured Films as an Immobilization Method vs. the Carbon Paste Classical Method

et al. 2012

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Tyrosinase-based biosensors containing a phthalocyanine as electron mediator have been prepared by two different methods. In the first approach, the enzyme and the electron mediator have been immobilized in carbon paste electrodes. In the second method, they have been introduced in an arachidic acid Langmuir-Blodgett nanostructured film that provides a biomimetic environment. The sensing properties of non-nanostructured and nanostructured biosensors towards catechol, catechin and phenol have been analyzed and compared. The enzyme retains the biocatalytic properties in both matrixes. However, the nanostructured biomimetic films show higher values of maximum reaction rates and lowest apparent Michaelis-Menten constants. In both types of sensors, the sensitivity follows the decreasing order catechol > catechin > phenol. The detection limits observed are in the range of 1.8-5.4 μM for Langmuir-Blodgett biosensors and 8.19-8.57 μM for carbon paste biosensors. In summary, it has been demonstrated that the Langmuir-Blodgett films provide a biomimetic environment and nanostructured biosensors show better performances in terms of kinetic, detection limit and stability. OPEN ACCESSCatalysts 2012, 2 518

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Section: Electrochemical Response Towards Phenolssupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Advantages of the Biomimetic Nanostructured Films as an Immobilization Method vs. the Carbon Paste Classical Method

et al. 2012

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“…According to previous experience [15], for determination of tyrosinase activity the substrates were immersed in 25 ml of tertbutylcatechol (4-TBC, 5 mmol l −1 , pH 7.0), l-DOPA (1 mmol l −1 , pH 7.0), o-aminophenol (1 mmol l −1 , pH 7.0) and l-Tyr (1 mmol l −1 , pH 7.0) and incubated at 30 • C, under continuous solution stirring. The concentration of reagents ensured optimum saturation of the enzyme with substrate and was successfully used in experiment acted as reagent.…”

Section: Sample Preparation and Measurementsmentioning

confidence: 99%

“…Continuing our interest in chemistry of conducting and sensing materials fabrication [15,16] we present here the results of research of sensing properties of tyrosinase from Agaricus bisporus adsorbed into phospholipid LS film, including conducting derivative of thianthrene, Fig. 1.…”

Section: Introductionmentioning

confidence: 99%

Active Langmuir–Schaefer films of tyrosinase—Characteristic

Cabaj

Sołoducho

Świst

2010

Sensors and Actuators B: Chemical

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“…11,12 4392 apetrei and apetrei have been developed using tyrosinase immobilized on different electrode supports. Different types of biosensors can be found in the literature, including carbon paste biosensors, 13 conducting polymer-modified electrodes, 12,14 biosensors based on silica sol-gel composite films, 15 Langmuir-Blodgett thin films, 16,17 and layer-by-layer films. 18 Use of a biocompatible matrix for tyrosine immobilization is essential for maintaining the functionality of the enzyme while providing accessibility to the target analyte.…”

Section: Introductionmentioning

confidence: 99%

Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine

Apetrei¹,

Apetrei²

2013

IJN

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A biosensor comprising tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode has been developed. The sensitive element, ie, tyrosinase, was immobilized using a drop-and-dry method followed by cross-linking. Tyrosinase maintained high bioactivity on this nanomaterial, catalyzing the oxidation of epinephrine to epinephrine-quinone, which was electrochemically reduced (−0.07 V versus Ag/AgCl) on the biosensor surface. Under optimum conditions, the biosensor showed a linear response in the range of 10–110 μM. The limit of detection was calculated to be 2.54 μM with a correlation coefficient of 0.977. The repeatability, expressed as the relative standard deviation for five consecutive determinations of 10 −5 M epinephrine solution was 3.4%. A good correlation was obtained between results obtained by the biosensor and those obtained by ultraviolet spectrophotometric methods.

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Langmuir–Blodgett film based biosensor for estimation of phenol derivatives

Cited by 48 publications

References 31 publications

Advantages of the Biomimetic Nanostructured Films as an Immobilization Method vs. the Carbon Paste Classical Method

Advantages of the Biomimetic Nanostructured Films as an Immobilization Method vs. the Carbon Paste Classical Method

Active Langmuir–Schaefer films of tyrosinase—Characteristic

Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine

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