haemorrhage in one animal; the problem was corrected laparoscopically. After surgery one animal died from pneumonia. The remaining seven animals had no complications and were utilized one each at 0 and 1 days then at 1, 2, 3, 4 and 6 weeks. The median serum creatinine values were similar (11.5 and 12 mg/L; P = 0.39) before and at the follow-up (at death). However, the peripheral renin activity before surgery (0.25 µ g/L per h) was lower than afterward, at 0.9 µ g/L per h ( P = 0.047). An ex vivo angiogram after death showed a widely patent, normal-appearing aorto-left renal artery anastomosis in all animals. On histopathology the early left renal parenchymal specimens showed transient and mild acute tubular necrosis that resolved over sequential specimens with no significant long-term sequelae.
LAPAROSCOPIC SPLENO-RENAL BYPASSMore recently, this extra-anatomical technique of renal revascularization was also evaluated in the laboratory [3]. A spleno-renal bypass was created in six mongrel dogs; particularly in the canine model and unlike in the clinical scenario, there is a significant disparity in the calibre of the splenic and renal arteries. Moreover, the small diameter of the distal splenic artery (2.2-2.5 mm) made this laparoscopic end-to-end anastomosis an especially technically challenging task. Briefly, the surgical technique comprised dissection of the distal segment of splenic artery and main left renal artery, transection and spatulation of the splenic artery and left renal artery, in-situ intracorporeal renal hypothermia, end-to-end splenorenal anastomosis, and unclamping and renal revascularization. In-situ transarterial renal hypothermia was achieved using a Pruit balloon catheter, as described above (Fig. 1). To overcome the limitations related to laparoscopic free-hand suturing in this 'microsurgical' task, we paid particular attention to the following steps: optimal port placement, use of 3 mm needle-scopic instruments, synchronization of hand movements with the animal's respiratory movements, and use of 5-0 polypropylene suture on an RB-2 needle. All procedures were completed laparoscopically with no open conversion. On in vivo / ex vivo renal arteriograms after surgery the anastomosis was patent in four animals, while in two there was obstruction of the anastomotic site.
LAPAROSCOPIC RENAL AUTOTRANSPLANTATIONThe feasibility of laparoscopic renal autotransplantation completely intracorporeally has been evaluated [4]. After laparoscopic left donor nephrectomy, renal hypothermia was achieved by in-situ intraarterial perfusion of ice-cold solution. The iliac artery and vein were previously dissected in preparation for autotransplantation. Laparoscopic vascular clamps were used to individually control the iliac artery and vein. The kidney was carefully positioned and stabilized in the pelvis. Individual end-to-side anastomosis of the renal artery and vein to the common iliac artery and vein were performed precisely (Fig. 2). The mean (range) operative duration was 6.2 (5.3-7.9) h, for venous anastom...